Restriction map for
pFN21AE6459
(Linear) (Six-base) MAPSORT of: KIEE6459.seq Check: 9909 from: 1 to: 1359
KIEE6459.orf
With 182 enzymes: SgfI *
March 16, 2015 11:32 ..
AatII G_ACGT'C
Cuts at: 0 1145 1359
Size: 1145 214
AccI GT'mk_AC
Cuts at: 0 326 1359
Size: 326 1033
Acc65I G'GTAC_C
Cuts at: 0 848 1359
Size: 848 511
AclI AA'CG_TT
Cuts at: 0 218 1359
Size: 218 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 213 414 637 1266 1359
Size: 194 19 201 223 629 93
Fragments arranged by size:
629 223 201 194 93 19
AflIII A'CryG_T
Cuts at: 0 1241 1359
Size: 1241 118
AlwNI CAG_nnn'CTG
Cuts at: 0 1038 1359
Size: 1038 321
ApaI G_GGCC'C
Cuts at: 0 1154 1210 1359
Size: 1154 56 149
Fragments arranged by size:
1154 149 56
ApoI r'AATT_y
Cuts at: 0 393 787 1359
Size: 393 394 572
Fragments arranged by size:
572 394 393
AvaI C'yCGr_G
Cuts at: 0 1010 1097 1359
Size: 1010 87 262
Fragments arranged by size:
1010 262 87
BamHI G'GATC_C
Cuts at: 0 607 1359
Size: 607 752
BanI G'GyrC_C
Cuts at: 0 848 1359
Size: 848 511
BanII G_rGCy'C
Cuts at: 0 95 1080 1154 1178 1210 1359
Size: 95 985 74 24 32 149
Fragments arranged by size:
985 149 95 74 32 24
BbvCI CC'TCA_GC
Cuts at: 0 388 445 1359
Size: 388 57 914
Fragments arranged by size:
914 388 57
BfrBI ATG'CAT
Cuts at: 0 147 1359
Size: 147 1212
BlpI GC'TnA_GC
Cuts at: 0 1039 1061 1359
Size: 1039 22 298
Fragments arranged by size:
1039 298 22
Bme1580I G_kGCm'C
Cuts at: 0 1154 1210 1359
Size: 1154 56 149
Fragments arranged by size:
1154 149 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 916 1240 1359
Size: 234 682 324 119
Fragments arranged by size:
682 324 234 119
Bpu10I CC'TnA_GC
Cuts at: 0 388 445 921 1327 1359
Size: 388 57 476 406 32
Fragments arranged by size:
476 406 388 57 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1359
Size: 631 728
BsaAI yAC'GTr
Cuts at: 0 1244 1359
Size: 1244 115
BsaHI Gr'CG_yC
Cuts at: 0 1142 1340 1359
Size: 1142 198 19
Fragments arranged by size:
1142 198 19
BsaWI w'CCGG_w
Cuts at: 0 101 1359
Size: 101 1258
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 841 871 1359
Size: 268 30 543 30 488
Fragments arranged by size:
543 488 268 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1184 1359
Size: 1184 175
BseYI C'CCAG_C
Cuts at: 0 496 1085 1165 1203 1359
Size: 496 589 80 38 156
Fragments arranged by size:
589 496 156 80 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 649 925 979 1359
Size: 524 125 276 54 380
Fragments arranged by size:
524 380 276 125 54
BsiEI CG_ry'CG
Cuts at: 0 78 1359
Size: 78 1281
BsiHKAI G_wGCw'C
Cuts at: 0 95 1249 1359
Size: 95 1154 110
Fragments arranged by size:
1154 110 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 1359
Size: 119 1240
Bsp1286I G_dGCh'C
Cuts at: 0 95 1080 1154 1178 1210 1249 1359
Size: 95 985 74 24 32 39 110
Fragments arranged by size:
985 110 95 74 39 32 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 968 1100 1359
Size: 968 132 259
Fragments arranged by size:
968 259 132
BsrBI CCG'CTC
Cuts at: 0 42 879 1359
Size: 42 837 480
Fragments arranged by size:
837 480 42
BsrDI GCAATG_nn'
Cuts at: 0 318 1302 1359
Size: 318 984 57
Fragments arranged by size:
984 318 57
BsrFI r'CCGG_y
Cuts at: 0 926 1359
Size: 926 433
BsrGI T'GTAC_A
Cuts at: 0 363 1359
Size: 363 996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 1359
Size: 267 1092
BstYI r'GATC_y
Cuts at: 0 607 1359
Size: 607 752
BstZ17I GTA'TAC
Cuts at: 0 327 1359
Size: 327 1032
Bsu36I CC'TnA_GG
Cuts at: 0 824 1270 1359
Size: 824 446 89
Fragments arranged by size:
824 446 89
BtgI C'CryG_G
Cuts at: 0 60 153 377 1170 1359
Size: 60 93 224 793 189
Fragments arranged by size:
793 224 189 93 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 966 1359
Size: 966 393
BtsI GCAGTG_nn'
Cuts at: 0 507 913 1359
Size: 507 406 446
Fragments arranged by size:
507 446 406
DrdI GACnn_nn'nnGTC
Cuts at: 0 1345 1359
Size: 1345 14
EaeI y'GGCC_r
Cuts at: 0 75 86 156 257 1359
Size: 75 11 70 101 1102
Fragments arranged by size:
1102 101 75 70 11
EagI C'GGCC_G
Cuts at: 0 75 1359
Size: 75 1284
EarI CTCTTCn'nnn_
Cuts at: 0 100 689 898 1359
Size: 100 589 209 461
Fragments arranged by size:
589 461 209 100
EcoICRI GAG'CTC
Cuts at: 0 93 1359
Size: 93 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 213 234 414 637 916 1240 1266
Size: 194 19 21 180 223 279 324 26
Cuts at: 1266 1359
Size: 93
Fragments arranged by size:
324 279 223 194 180 93 26 21
19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 616 1030 1359
Size: 292 324 414 329
Fragments arranged by size:
414 329 324 292
EcoO109I rG'GnC_Cy
Cuts at: 0 36 1089 1150 1151 1159 1207 1359
Size: 36 1053 61 1 8 48 152
Fragments arranged by size:
1053 152 61 48 36 8 1
FspI TGC'GCA
Cuts at: 0 679 1359
Size: 679 680
FspAI rTGC'GCAy
Cuts at: 0 679 1359
Size: 679 680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 742 774 856 888 904 936
Size: 568 32 142 32 82 32 16 32
Cuts at: 936 949 981 1004 1036 1359
Size: 13 32 23 32 323
Fragments arranged by size:
568 323 142 82 32 32 32 32
32 32 23 16 13
KpnI G_GTAC'C
Cuts at: 0 852 1359
Size: 852 507
MscI TGG'CCA
Cuts at: 0 259 1359
Size: 259 1100
MslI CAynn'nnrTG
Cuts at: 0 152 382 1359
Size: 152 230 977
Fragments arranged by size:
977 230 152
MspA1I CmG'CkG
Cuts at: 0 62 185 1359
Size: 62 123 1174
Fragments arranged by size:
1174 123 62
NaeI GCC'GGC
Cuts at: 0 928 1359
Size: 928 431
NcoI C'CATG_G
Cuts at: 0 153 1170 1359
Size: 153 1017 189
Fragments arranged by size:
1017 189 153
NgoMIV G'CCGG_C
Cuts at: 0 926 1359
Size: 926 433
NsiI A_TGCA'T
Cuts at: 0 149 1359
Size: 149 1210
PasI CC'CwG_GG
Cuts at: 0 288 1147 1359
Size: 288 859 212
Fragments arranged by size:
859 288 212
PfoI T'CCnGG_A
Cuts at: 0 335 1359
Size: 335 1024
PmlI CAC'GTG
Cuts at: 0 1244 1359
Size: 1244 115
PpuMI rG'GwC_Cy
Cuts at: 0 36 1089 1159 1359
Size: 36 1053 70 200
Fragments arranged by size:
1053 200 70 36
PspOMI G'GGCC_C
Cuts at: 0 1150 1206 1359
Size: 1150 56 153
Fragments arranged by size:
1150 153 56
PspXI vC'TCGA_Gb
Cuts at: 0 1010 1359
Size: 1010 349
SacI G_AGCT'C
Cuts at: 0 95 1359
Size: 95 1264
SacII CC_GC'GG
Cuts at: 0 63 1359
Size: 63 1296
SanDI GG'GwC_CC
Cuts at: 0 36 1359
Size: 36 1323
SapI GCTCTTCn'nnn_
Cuts at: 0 100 898 1359
Size: 100 798 461
Fragments arranged by size:
798 461 100
SmlI C'TyrA_G
Cuts at: 0 646 1010 1359
Size: 646 364 349
Fragments arranged by size:
646 364 349
StuI AGG'CCT
Cuts at: 0 1127 1359
Size: 1127 232
StyI C'CwwG_G
Cuts at: 0 29 153 1170 1359
Size: 29 124 1017 189
Fragments arranged by size:
1017 189 124 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 929 1305 1359
Size: 929 376 54
Fragments arranged by size:
929 376 54
TatI w'GTAC_w
Cuts at: 0 363 588 1359
Size: 363 225 771
Fragments arranged by size:
771 363 225
XhoI C'TCGA_G
Cuts at: 0 1010 1359
Size: 1010 349
ZraI GAC'GTC
Cuts at: 0 1143 1359
Size: 1143 216
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbvCI BfrBI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FspI FspAI Hin4I KpnI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI PasI PfoI
PmlI PpuMI PspOMI PspXI SacI SacII SanDI SapI
SmlI StuI StyI TaqII TatI XhoI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BcgI BciVI BclI BglI BglII BmgBI BmrI BmtI
BplI BsaI BsaBI BsiWI BsmI BspEI BspHI BssHII
BssSI BstAPI BstBI BstEII ClaI CspCI DraI DraIII
EciI EcoRI EcoRV FalI FseI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NspI PacI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XbaI XcmI XmaI XmnI