Restriction map for
pFN21AE6456
(Linear) (Six-base) MAPSORT of: KIEE6456.seq Check: 2615 from: 1 to: 1659
KIEE6456.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1235 1659
Size: 1235 424
AccI GT'mk_AC
Cuts at: 0 1583 1659
Size: 1583 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1659
Size: 1114 545
AflIII A'CryG_T
Cuts at: 0 556 1659
Size: 556 1103
AhdI GACnn_n'nnGTC
Cuts at: 0 905 1659
Size: 905 754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1354 1659
Size: 1354 305
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 1659
Size: 695 32 932
Fragments arranged by size:
932 695 32
AlwNI CAG_nnn'CTG
Cuts at: 0 133 797 1351 1659
Size: 133 664 554 308
Fragments arranged by size:
664 554 308 133
ApaI G_GGCC'C
Cuts at: 0 19 634 655 679 1266 1659
Size: 19 615 21 24 587 393
Fragments arranged by size:
615 587 393 24 21 19
ApoI r'AATT_y
Cuts at: 0 261 1659
Size: 261 1398
AvaI C'yCGr_G
Cuts at: 0 691 809 1606 1659
Size: 691 118 797 53
Fragments arranged by size:
797 691 118 53
AvrII C'CTAG_G
Cuts at: 0 1321 1659
Size: 1321 338
BamHI G'GATC_C
Cuts at: 0 889 1659
Size: 889 770
BanI G'GyrC_C
Cuts at: 0 37 1034 1437 1659
Size: 37 997 403 222
Fragments arranged by size:
997 403 222 37
BanII G_rGCy'C
Cuts at: 0 19 177 379 634 655 679 844 987
Size: 19 158 202 255 21 24 165 143
Cuts at: 987 1266 1607 1659
Size: 279 341 52
Fragments arranged by size:
341 279 255 202 165 158 143 52
24 21 19
BbeI G_GCGC'C
Cuts at: 0 1038 1659
Size: 1038 621
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 1521 1659
Size: 52 1469 138
Fragments arranged by size:
1469 138 52
BglI GCCn_nnn'nGGC
Cuts at: 0 1522 1659
Size: 1522 137
BlpI GC'TnA_GC
Cuts at: 0 71 1613 1659
Size: 71 1542 46
Fragments arranged by size:
1542 71 46
Bme1580I G_kGCm'C
Cuts at: 0 19 634 655 679 750 1266 1304 1440
Size: 19 615 21 24 71 516 38 136
Cuts at: 1440 1465 1659
Size: 25 194
Fragments arranged by size:
615 516 194 136 71 38 25 24
21 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 13 717 1189 1566 1659
Size: 13 704 472 377 93
Fragments arranged by size:
704 472 377 93 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1659
Size: 1276 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1659
Size: 915 744
BsaI GGTCTCn'nnnn_
Cuts at: 0 841 1659
Size: 841 818
BsaHI Gr'CG_yC
Cuts at: 0 1035 1659
Size: 1035 624
BsaWI w'CCGG_w
Cuts at: 0 1077 1659
Size: 1077 582
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 974 1004 1659
Size: 974 30 655
Fragments arranged by size:
974 655 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 1012 1096 1659
Size: 177 835 84 563
Fragments arranged by size:
835 563 177 84
BseYI C'CCAG_C
Cuts at: 0 11 437 473 485 635 1065 1166 1247
Size: 11 426 36 12 150 430 101 81
Cuts at: 1247 1414 1659
Size: 167 245
Fragments arranged by size:
430 426 245 167 150 101 81 36
12 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 667 1468 1659
Size: 565 102 801 191
Fragments arranged by size:
801 565 191 102
BsiHKAI G_wGCw'C
Cuts at: 0 924 1659
Size: 924 735
BsmBI CGTCTCn'nnnn_
Cuts at: 0 544 1659
Size: 544 1115
Bsp1286I G_dGCh'C
Cuts at: 0 19 177 379 634 655 679 750 844
Size: 19 158 202 255 21 24 71 94
Cuts at: 844 924 987 1266 1304 1440 1465 1607 1659
Size: 80 63 279 38 136 25 142 52
Fragments arranged by size:
279 255 202 158 142 136 94 80
71 63 52 38 25 24 21 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 895 1235 1569 1659
Size: 895 340 334 90
Fragments arranged by size:
895 340 334 90
BsrBI CCG'CTC
Cuts at: 0 70 1659
Size: 70 1589
BsrFI r'CCGG_y
Cuts at: 0 1334 1521 1659
Size: 1334 187 138
Fragments arranged by size:
1334 187 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 938 1052 1401 1659
Size: 938 114 349 258
Fragments arranged by size:
938 349 258 114
BstYI r'GATC_y
Cuts at: 0 889 1659
Size: 889 770
Bsu36I CC'TnA_GG
Cuts at: 0 113 1130 1659
Size: 113 1017 529
Fragments arranged by size:
1017 529 113
BtgI C'CryG_G
Cuts at: 0 931 1659
Size: 931 728
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 15 433 1659
Size: 15 418 1226
Fragments arranged by size:
1226 418 15
BtsI GCAGTG_nn'
Cuts at: 0 218 228 1020 1659
Size: 218 10 792 639
Fragments arranged by size:
792 639 218 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 211 1579 1659
Size: 211 1368 80
Fragments arranged by size:
1368 211 80
EaeI y'GGCC_r
Cuts at: 0 1475 1631 1659
Size: 1475 156 28
Fragments arranged by size:
1475 156 28
EarI CTCTTCn'nnn_
Cuts at: 0 314 1659
Size: 314 1345
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 995 1659
Size: 995 664
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1276 1659
Size: 1114 162 383
Fragments arranged by size:
1114 383 162
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1659
Size: 52 1607
EcoO109I rG'GnC_Cy
Cuts at: 0 15 213 353 515 630 631 651 652
Size: 15 198 140 162 115 1 20 1
Cuts at: 652 675 676 907 1041 1089 1318 1325 1425
Size: 23 1 231 134 48 229 7 100
Cuts at: 1425 1510 1659
Size: 85 149
Fragments arranged by size:
231 229 198 162 149 140 134 115
100 85 48 23 20 15 7 1
1 1
EcoRI G'AATT_C
Cuts at: 0 261 1659
Size: 261 1398
HaeII r_GCGC'y
Cuts at: 0 1038 1659
Size: 1038 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 164 196 891 923 1265 1297 1414 1446
Size: 164 32 695 32 342 32 117 32
Cuts at: 1446 1659
Size: 213
Fragments arranged by size:
695 342 213 164 117 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1034 1659
Size: 1034 625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1659
Size: 623 1036
MscI TGG'CCA
Cuts at: 0 1633 1659
Size: 1633 26
MspA1I CmG'CkG
Cuts at: 0 188 579 784 875 969 1418 1655 1659
Size: 188 391 205 91 94 449 237 4
Fragments arranged by size:
449 391 237 205 188 94 91 4
NaeI GCC'GGC
Cuts at: 0 1336 1523 1659
Size: 1336 187 136
Fragments arranged by size:
1336 187 136
NarI GG'CG_CC
Cuts at: 0 1035 1659
Size: 1035 624
NcoI C'CATG_G
Cuts at: 0 931 1659
Size: 931 728
NgoMIV G'CCGG_C
Cuts at: 0 1334 1521 1659
Size: 1334 187 138
Fragments arranged by size:
1334 187 138
NspI r_CATG'y
Cuts at: 0 560 1345 1659
Size: 560 785 314
Fragments arranged by size:
785 560 314
PasI CC'CwG_GG
Cuts at: 0 310 350 938 1659
Size: 310 40 588 721
Fragments arranged by size:
721 588 310 40
PciI A'CATG_T
Cuts at: 0 556 1659
Size: 556 1103
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 1659
Size: 485 1174
PpuMI rG'GwC_Cy
Cuts at: 0 213 515 907 1659
Size: 213 302 392 752
Fragments arranged by size:
752 392 302 213
PshAI GACnn'nnGTC
Cuts at: 0 594 1659
Size: 594 1065
PspOMI G'GGCC_C
Cuts at: 0 15 630 651 675 1262 1659
Size: 15 615 21 24 587 397
Fragments arranged by size:
615 587 397 24 21 15
PspXI vC'TCGA_Gb
Cuts at: 0 691 809 1659
Size: 691 118 850
Fragments arranged by size:
850 691 118
PstI C_TGCA'G
Cuts at: 0 187 293 344 398 806 1333 1659
Size: 187 106 51 54 408 527 326
Fragments arranged by size:
527 408 326 187 106 54 51
PvuII CAG'CTG
Cuts at: 0 579 784 875 969 1418 1655 1659
Size: 579 205 91 94 449 237 4
Fragments arranged by size:
579 449 237 205 94 91 4
SanDI GG'GwC_CC
Cuts at: 0 515 1659
Size: 515 1144
SfcI C'TryA_G
Cuts at: 0 183 289 340 366 394 802 1329 1659
Size: 183 106 51 26 28 408 527 330
Fragments arranged by size:
527 408 330 183 106 51 28 26
SfoI GGC'GCC
Cuts at: 0 1036 1659
Size: 1036 623
SmlI C'TyrA_G
Cuts at: 0 691 809 894 1659
Size: 691 118 85 765
Fragments arranged by size:
765 691 118 85
SphI G_CATG'C
Cuts at: 0 1345 1659
Size: 1345 314
StyI C'CwwG_G
Cuts at: 0 931 1321 1506 1659
Size: 931 390 185 153
Fragments arranged by size:
931 390 185 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1138 1393 1659
Size: 1138 255 266
Fragments arranged by size:
1138 266 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 609 641 974 1006 1659
Size: 609 32 333 32 653
Fragments arranged by size:
653 609 333 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1558 1659
Size: 1558 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 1027 1231 1659
Size: 48 979 204 428
Fragments arranged by size:
979 428 204 48
XhoI C'TCGA_G
Cuts at: 0 691 809 1659
Size: 691 118 850
Fragments arranged by size:
850 691 118
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I KasI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PpuMI PshAI PspOMI PspXI PstI PvuII SanDI SfcI
SfoI SmlI SphI StyI TaqII TstI Tth111I XcmI
XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApaLI
AscI AseI AsiSI BaeI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII EagI EcoICRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NdeI
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI
XmaI XmnI ZraI