Restriction map for
pFN21AE6452
(Linear) (Six-base) MAPSORT of: KIEE6452.seq Check: 6670 from: 1 to: 1458
KIEE6452.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 25 1151 1458
Size: 25 1126 307
Fragments arranged by size:
1126 307 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 396 964 1458
Size: 343 53 568 494
Fragments arranged by size:
568 494 343 53
AfeI AGC'GCT
Cuts at: 0 1139 1458
Size: 1139 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 720 1346 1458
Size: 720 626 112
Fragments arranged by size:
720 626 112
AlwNI CAG_nnn'CTG
Cuts at: 0 1441 1458
Size: 1441 17
BamHI G'GATC_C
Cuts at: 0 176 1458
Size: 176 1282
BanI G'GyrC_C
Cuts at: 0 466 571 822 1072 1458
Size: 466 105 251 250 386
Fragments arranged by size:
466 386 251 250 105
BanII G_rGCy'C
Cuts at: 0 21 307 995 1186 1458
Size: 21 286 688 191 272
Fragments arranged by size:
688 286 272 191 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 1458
Size: 656 802
Bme1580I G_kGCm'C
Cuts at: 0 699 1458
Size: 699 759
BmrI ACTGGGnnnn_n'
Cuts at: 0 1087 1311 1458
Size: 1087 224 147
Fragments arranged by size:
1087 224 147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 1458
Size: 547 911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 368 599 1458
Size: 20 348 231 859
Fragments arranged by size:
859 348 231 20
Bpu10I CC'TnA_GC
Cuts at: 0 301 1349 1458
Size: 301 1048 109
Fragments arranged by size:
1048 301 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 18 48 1458
Size: 18 30 1410
Fragments arranged by size:
1410 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1231 1458
Size: 1231 227
BseYI C'CCAG_C
Cuts at: 0 47 267 1458
Size: 47 220 1191
Fragments arranged by size:
1191 220 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1458
Size: 75 1383
BsiHKAI G_wGCw'C
Cuts at: 0 21 216 307 1458
Size: 21 195 91 1151
Fragments arranged by size:
1151 195 91 21
BsmI GAATG_Cn'
Cuts at: 0 1234 1458
Size: 1234 224
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 1458
Size: 777 681
Bsp1286I G_dGCh'C
Cuts at: 0 21 216 307 699 995 1186 1458
Size: 21 195 91 392 296 191 272
Fragments arranged by size:
392 296 272 195 191 91 21
BsrDI GCAATG_nn'
Cuts at: 0 1328 1458
Size: 1328 130
BsrFI r'CCGG_y
Cuts at: 0 1216 1458
Size: 1216 242
BssSI C'ACGA_G
Cuts at: 0 315 1036 1458
Size: 315 721 422
Fragments arranged by size:
721 422 315
BstYI r'GATC_y
Cuts at: 0 176 1458
Size: 176 1282
BstZ17I GTA'TAC
Cuts at: 0 1152 1458
Size: 1152 306
BtgI C'CryG_G
Cuts at: 0 395 977 1458
Size: 395 582 481
Fragments arranged by size:
582 481 395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1176 1458
Size: 1176 282
BtsI GCAGTG_nn'
Cuts at: 0 1172 1226 1442 1458
Size: 1172 54 216 16
Fragments arranged by size:
1172 216 54 16
DraIII CAC_nnn'GTG
Cuts at: 0 1167 1458
Size: 1167 291
EcoICRI GAG'CTC
Cuts at: 0 19 305 1458
Size: 19 286 1153
Fragments arranged by size:
1153 286 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 343 368 396 599 964 1458
Size: 20 323 25 28 203 365 494
Fragments arranged by size:
494 365 323 203 28 25 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 579 1458
Size: 579 879
HaeII r_GCGC'y
Cuts at: 0 1141 1458
Size: 1141 317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1458
Size: 822 636
MslI CAynn'nnrTG
Cuts at: 0 918 1458
Size: 918 540
NcoI C'CATG_G
Cuts at: 0 395 1458
Size: 395 1063
PflMI CCAn_nnn'nTGG
Cuts at: 0 692 1458
Size: 692 766
PsiI TTA'TAA
Cuts at: 0 1297 1458
Size: 1297 161
PstI C_TGCA'G
Cuts at: 0 802 1233 1358 1458
Size: 802 431 125 100
Fragments arranged by size:
802 431 125 100
SacI G_AGCT'C
Cuts at: 0 21 307 1458
Size: 21 286 1151
Fragments arranged by size:
1151 286 21
ScaI AGT'ACT
Cuts at: 0 409 1458
Size: 409 1049
SfcI C'TryA_G
Cuts at: 0 798 1229 1354 1430 1446 1458
Size: 798 431 125 76 16 12
Fragments arranged by size:
798 431 125 76 16 12
StyI C'CwwG_G
Cuts at: 0 395 1458
Size: 395 1063
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 11 259 943 1203 1458
Size: 11 248 684 260 255
Fragments arranged by size:
684 260 255 248 11
TatI w'GTAC_w
Cuts at: 0 407 731 815 1458
Size: 407 324 84 643
Fragments arranged by size:
643 407 324 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 44 1110 1374 1458
Size: 44 1066 264 84
Fragments arranged by size:
1066 264 84 44
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI BamHI BanI BanII
BbsI Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrFI
BssSI BstYI BstZ17I BtgI BtgZI BtsI DraIII EcoICRI
Eco57MI EcoNI HaeII MmeI MslI NcoI PflMI PsiI
PstI SacI ScaI SfcI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI
Bsu36I ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI