Restriction map for
pFN21AE6451
(Linear) (Six-base) MAPSORT of: KIEE6451.seq Check: 9195 from: 1 to: 1512
KIEE6451.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1512
Size: 1397 115
AfeI AGC'GCT
Cuts at: 0 508 1512
Size: 508 1004
AlwNI CAG_nnn'CTG
Cuts at: 0 498 501 1339 1512
Size: 498 3 838 173
Fragments arranged by size:
838 498 173 3
ApaI G_GGCC'C
Cuts at: 0 34 217 604 1512
Size: 34 183 387 908
Fragments arranged by size:
908 387 183 34
ApaLI G'TGCA_C
Cuts at: 0 741 1512
Size: 741 771
AscI GG'CGCG_CC
Cuts at: 0 904 1118 1512
Size: 904 214 394
Fragments arranged by size:
904 394 214
AvaI C'yCGr_G
Cuts at: 0 592 793 1451 1512
Size: 592 201 658 61
Fragments arranged by size:
658 592 201 61
BanI G'GyrC_C
Cuts at: 0 67 719 919 1030 1512
Size: 67 652 200 111 482
Fragments arranged by size:
652 482 200 111 67
BanII G_rGCy'C
Cuts at: 0 34 217 332 548 604 800 995 1394
Size: 34 183 115 216 56 196 195 399
Cuts at: 1394 1463 1512
Size: 69 49
Fragments arranged by size:
399 216 196 195 183 115 69 56
49 34
BbeI G_GCGC'C
Cuts at: 0 71 723 923 1034 1512
Size: 71 652 200 111 478
Fragments arranged by size:
652 478 200 111 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 1266 1512
Size: 114 1152 246
Fragments arranged by size:
1152 246 114
BbvCI CC'TCA_GC
Cuts at: 0 1370 1512
Size: 1370 142
BglI GCCn_nnn'nGGC
Cuts at: 0 571 594 717 982 1512
Size: 571 23 123 265 530
Fragments arranged by size:
571 530 265 123 23
BlpI GC'TnA_GC
Cuts at: 0 1444 1512
Size: 1444 68
Bme1580I G_kGCm'C
Cuts at: 0 34 217 604 745 851 1512
Size: 34 183 387 141 106 661
Fragments arranged by size:
661 387 183 141 106 34
BmgBI CAC'GTC
Cuts at: 0 954 1512
Size: 954 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 1127 1512
Size: 1127 385
BmtI G_CTAG'C
Cuts at: 0 196 1512
Size: 196 1316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 523 1447 1456 1512
Size: 346 177 924 9 56
Fragments arranged by size:
924 346 177 56 9
Bpu10I CC'TnA_GC
Cuts at: 0 1003 1370 1512
Size: 1003 367 142
Fragments arranged by size:
1003 367 142
BsaHI Gr'CG_yC
Cuts at: 0 68 152 720 860 920 1031 1512
Size: 68 84 568 140 60 111 481
Fragments arranged by size:
568 481 140 111 84 68 60
BseYI C'CCAG_C
Cuts at: 0 47 53 63 343 399 1140 1267 1512
Size: 47 6 10 280 56 741 127 245
Fragments arranged by size:
741 280 245 127 56 47 10 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1512
Size: 538 974
BsiEI CG_ry'CG
Cuts at: 0 610 633 750 949 1200 1512
Size: 610 23 117 199 251 312
Fragments arranged by size:
610 312 251 199 117 23
BsiHKAI G_wGCw'C
Cuts at: 0 332 353 745 1103 1178 1445 1512
Size: 332 21 392 358 75 267 67
Fragments arranged by size:
392 358 332 267 75 67 21
Bsp1286I G_dGCh'C
Cuts at: 0 34 217 332 353 548 604 745 800
Size: 34 183 115 21 195 56 141 55
Cuts at: 800 851 995 1103 1178 1394 1445 1463 1512
Size: 51 144 108 75 216 51 18 49
Fragments arranged by size:
216 195 183 144 141 115 108 75
56 55 51 51 49 34 21 18
BspHI T'CATG_A
Cuts at: 0 578 1512
Size: 578 934
BsrBI CCG'CTC
Cuts at: 0 417 486 1512
Size: 417 69 1026
Fragments arranged by size:
1026 417 69
BsrFI r'CCGG_y
Cuts at: 0 85 154 625 716 1195 1246 1512
Size: 85 69 471 91 479 51 266
Fragments arranged by size:
479 471 266 91 85 69 51
BssHII G'CGCG_C
Cuts at: 0 131 904 1016 1018 1082 1118 1512
Size: 131 773 112 2 64 36 394
Fragments arranged by size:
773 394 131 112 64 36 2
BssSI C'ACGA_G
Cuts at: 0 232 1156 1512
Size: 232 924 356
Fragments arranged by size:
924 356 232
BstEII G'GTnAC_C
Cuts at: 0 81 1512
Size: 81 1431
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 819 1140 1512
Size: 441 378 321 372
Fragments arranged by size:
441 378 372 321
BtgI C'CryG_G
Cuts at: 0 287 1166 1512
Size: 287 879 346
Fragments arranged by size:
879 346 287
BtsI GCAGTG_nn'
Cuts at: 0 555 743 1512
Size: 555 188 769
Fragments arranged by size:
769 555 188
EaeI y'GGCC_r
Cuts at: 0 431 489 946 983 1197 1244 1512
Size: 431 58 457 37 214 47 268
Fragments arranged by size:
457 431 268 214 58 47 37
EagI C'GGCC_G
Cuts at: 0 946 1197 1512
Size: 946 251 315
Fragments arranged by size:
946 315 251
EarI CTCTTCn'nnn_
Cuts at: 0 805 1512
Size: 805 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 673 1361 1512
Size: 673 688 151
Fragments arranged by size:
688 673 151
EcoICRI GAG'CTC
Cuts at: 0 330 1512
Size: 330 1182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 523 1397 1447 1456 1512
Size: 346 177 874 50 9 56
Fragments arranged by size:
874 346 177 56 50 9
EcoO109I rG'GnC_Cy
Cuts at: 0 30 31 214 1431 1512
Size: 30 1 183 1217 81
Fragments arranged by size:
1217 183 81 30 1
HaeII r_GCGC'y
Cuts at: 0 8 71 510 626 723 793 923 1034
Size: 8 63 439 116 97 70 130 111
Cuts at: 1034 1451 1512
Size: 417 61
Fragments arranged by size:
439 417 130 116 111 97 70 63
61 8
KasI G'GCGC_C
Cuts at: 0 67 719 919 1030 1512
Size: 67 652 200 111 482
Fragments arranged by size:
652 482 200 111 67
MscI TGG'CCA
Cuts at: 0 433 985 1512
Size: 433 552 527
Fragments arranged by size:
552 527 433
MslI CAynn'nnrTG
Cuts at: 0 232 1512
Size: 232 1280
MspA1I CmG'CkG
Cuts at: 0 289 343 498 684 1168 1512
Size: 289 54 155 186 484 344
Fragments arranged by size:
484 344 289 186 155 54
NaeI GCC'GGC
Cuts at: 0 156 627 718 1197 1248 1512
Size: 156 471 91 479 51 264
Fragments arranged by size:
479 471 264 156 91 51
NarI GG'CG_CC
Cuts at: 0 68 720 920 1031 1512
Size: 68 652 200 111 481
Fragments arranged by size:
652 481 200 111 68
NgoMIV G'CCGG_C
Cuts at: 0 154 625 716 1195 1246 1512
Size: 154 471 91 479 51 266
Fragments arranged by size:
479 471 266 154 91 51
NheI G'CTAG_C
Cuts at: 0 192 1512
Size: 192 1320
NotI GC'GGCC_GC
Cuts at: 0 946 1512
Size: 946 566
PasI CC'CwG_GG
Cuts at: 0 701 1512
Size: 701 811
PfoI T'CCnGG_A
Cuts at: 0 323 1512
Size: 323 1189
PspOMI G'GGCC_C
Cuts at: 0 30 213 600 1512
Size: 30 183 387 912
Fragments arranged by size:
912 387 183 30
PstI C_TGCA'G
Cuts at: 0 683 1091 1512
Size: 683 408 421
Fragments arranged by size:
683 421 408
PvuII CAG'CTG
Cuts at: 0 498 684 1512
Size: 498 186 828
Fragments arranged by size:
828 498 186
RsrII CG'GwC_CG
Cuts at: 0 1183 1512
Size: 1183 329
SacI G_AGCT'C
Cuts at: 0 332 1512
Size: 332 1180
SacII CC_GC'GG
Cuts at: 0 290 1169 1512
Size: 290 879 343
Fragments arranged by size:
879 343 290
SapI GCTCTTCn'nnn_
Cuts at: 0 805 1512
Size: 805 707
SfcI C'TryA_G
Cuts at: 0 159 679 1087 1512
Size: 159 520 408 425
Fragments arranged by size:
520 425 408 159
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 982 1512
Size: 982 530
SfoI GGC'GCC
Cuts at: 0 69 721 921 1032 1512
Size: 69 652 200 111 480
Fragments arranged by size:
652 480 200 111 69
SgrAI Cr'CCGG_yG
Cuts at: 0 625 716 1512
Size: 625 91 796
Fragments arranged by size:
796 625 91
SmaI CCC'GGG
Cuts at: 0 594 1453 1512
Size: 594 859 59
Fragments arranged by size:
859 594 59
SrfI GCCC'GGGC
Cuts at: 0 594 1512
Size: 594 918
StuI AGG'CCT
Cuts at: 0 359 1512
Size: 359 1153
StyI C'CwwG_G
Cuts at: 0 833 1512
Size: 833 679
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 165 1512
Size: 45 120 1347
Fragments arranged by size:
1347 120 45
TatI w'GTAC_w
Cuts at: 0 407 1512
Size: 407 1105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 44 321 783 1512
Size: 44 277 462 729
Fragments arranged by size:
729 462 277 44
XmaI C'CCGG_G
Cuts at: 0 592 1451 1512
Size: 592 859 61
Fragments arranged by size:
859 592 61
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApaLI AscI AvaI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BsaHI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssHII BssSI BstEII
BstXI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII KasI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NotI PasI PfoI PspOMI PstI
PvuII RsrII SacI SacII SapI SfcI SfiI SfoI
SgrAI SmaI SrfI StuI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrGI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI SalI SanDI SbfI ScaI SexAI SgfI SmlI
SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI