Restriction map for
pFN21AE6446
(Linear) (Six-base) MAPSORT of: KIEE6446.seq Check: 1751 from: 1 to: 1515
KIEE6446.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 639 1515
Size: 639 876
Acc65I G'GTAC_C
Cuts at: 0 508 1515
Size: 508 1007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 828 1515
Size: 342 486 687
Fragments arranged by size:
687 486 342
AflIII A'CryG_T
Cuts at: 0 94 1196 1515
Size: 94 1102 319
Fragments arranged by size:
1102 319 94
AleI CACnn'nnGTG
Cuts at: 0 61 181 583 1515
Size: 61 120 402 932
Fragments arranged by size:
932 402 120 61
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 657 1515
Size: 397 260 858
Fragments arranged by size:
858 397 260
AlwNI CAG_nnn'CTG
Cuts at: 0 12 1515
Size: 12 1503
ApaI G_GGCC'C
Cuts at: 0 203 1515
Size: 203 1312
AvaI C'yCGr_G
Cuts at: 0 622 1515
Size: 622 893
BanI G'GyrC_C
Cuts at: 0 508 777 1036 1515
Size: 508 269 259 479
Fragments arranged by size:
508 479 269 259
BanII G_rGCy'C
Cuts at: 0 203 950 1095 1515
Size: 203 747 145 420
Fragments arranged by size:
747 420 203 145
BbeI G_GCGC'C
Cuts at: 0 1040 1515
Size: 1040 475
BbsI GAAGACnn'nnnn_
Cuts at: 0 513 762 1364 1515
Size: 513 249 602 151
Fragments arranged by size:
602 513 249 151
BbvCI CC'TCA_GC
Cuts at: 0 307 1515
Size: 307 1208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 613 647 1515
Size: 613 34 868
Fragments arranged by size:
868 613 34
Bme1580I G_kGCm'C
Cuts at: 0 203 1515
Size: 203 1312
BmgBI CAC'GTC
Cuts at: 0 1387 1515
Size: 1387 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 736 1515
Size: 736 779
BmtI G_CTAG'C
Cuts at: 0 905 1515
Size: 905 610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 508 522 1321 1416 1515
Size: 34 474 14 799 95 99
Fragments arranged by size:
799 474 99 95 34 14
Bpu10I CC'TnA_GC
Cuts at: 0 307 1515
Size: 307 1208
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 589 1515
Size: 145 444 926
Fragments arranged by size:
926 444 145
BsaAI yAC'GTr
Cuts at: 0 95 1515
Size: 95 1420
BsaHI Gr'CG_yC
Cuts at: 0 440 1037 1166 1397 1515
Size: 440 597 129 231 118
Fragments arranged by size:
597 440 231 129 118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 1419 1515
Size: 814 605 96
Fragments arranged by size:
814 605 96
BseYI C'CCAG_C
Cuts at: 0 32 252 384 1515
Size: 32 220 132 1131
Fragments arranged by size:
1131 220 132 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 228 617 1515
Size: 41 187 389 898
Fragments arranged by size:
898 389 187 41
BsiEI CG_ry'CG
Cuts at: 0 660 892 1515
Size: 660 232 623
Fragments arranged by size:
660 623 232
BsiHKAI G_wGCw'C
Cuts at: 0 188 992 1515
Size: 188 804 523
Fragments arranged by size:
804 523 188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 447 1515
Size: 447 1068
Bsp1286I G_dGCh'C
Cuts at: 0 188 203 950 992 1095 1515
Size: 188 15 747 42 103 420
Fragments arranged by size:
747 420 188 103 42 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 1515
Size: 639 876
BsrBI CCG'CTC
Cuts at: 0 1305 1515
Size: 1305 210
BsrDI GCAATG_nn'
Cuts at: 0 130 1515
Size: 130 1385
BsrFI r'CCGG_y
Cuts at: 0 404 891 1515
Size: 404 487 624
Fragments arranged by size:
624 487 404
BsrGI T'GTAC_A
Cuts at: 0 719 1515
Size: 719 796
BssSI C'ACGA_G
Cuts at: 0 991 1515
Size: 991 524
BstEII G'GTnAC_C
Cuts at: 0 63 1515
Size: 63 1452
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 585 1095 1515
Size: 426 159 510 420
Fragments arranged by size:
510 426 420 159
BstYI r'GATC_y
Cuts at: 0 530 1308 1515
Size: 530 778 207
Fragments arranged by size:
778 530 207
Bsu36I CC'TnA_GG
Cuts at: 0 859 1515
Size: 859 656
BtgI C'CryG_G
Cuts at: 0 59 1073 1515
Size: 59 1014 442
Fragments arranged by size:
1014 442 59
BtsI GCAGTG_nn'
Cuts at: 0 540 757 1515
Size: 540 217 758
Fragments arranged by size:
758 540 217
DraIII CAC_nnn'GTG
Cuts at: 0 801 1515
Size: 801 714
EaeI y'GGCC_r
Cuts at: 0 53 266 284 416 463 575 657 889
Size: 53 213 18 132 47 112 82 232
Cuts at: 889 1148 1459 1515
Size: 259 311 56
Fragments arranged by size:
311 259 232 213 132 112 82 56
53 47 18
EagI C'GGCC_G
Cuts at: 0 657 889 1515
Size: 657 232 626
Fragments arranged by size:
657 626 232
EarI CTCTTCn'nnn_
Cuts at: 0 1108 1234 1515
Size: 1108 126 281
Fragments arranged by size:
1108 281 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 391 1306 1515
Size: 391 915 209
Fragments arranged by size:
915 391 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 342 508 522 828 1321 1416 1515
Size: 34 308 166 14 306 493 95 99
Fragments arranged by size:
493 308 306 166 99 95 34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1431 1515
Size: 1431 84
EcoO109I rG'GnC_Cy
Cuts at: 0 9 72 199 200 863 1515
Size: 9 63 127 1 663 652
Fragments arranged by size:
663 652 127 63 9 1
FseI GG_CCGG'CC
Cuts at: 0 895 1515
Size: 895 620
HaeII r_GCGC'y
Cuts at: 0 675 1040 1515
Size: 675 365 475
Fragments arranged by size:
675 475 365
KasI G'GCGC_C
Cuts at: 0 1036 1515
Size: 1036 479
KpnI G_GTAC'C
Cuts at: 0 512 1515
Size: 512 1003
MscI TGG'CCA
Cuts at: 0 55 268 286 418 577 1461 1515
Size: 55 213 18 132 159 884 54
Fragments arranged by size:
884 213 159 132 55 54 18
MslI CAynn'nnrTG
Cuts at: 0 61 181 217 379 583 1515
Size: 61 120 36 162 204 932
Fragments arranged by size:
932 204 162 120 61 36
MspA1I CmG'CkG
Cuts at: 0 32 468 1222 1393 1515
Size: 32 436 754 171 122
Fragments arranged by size:
754 436 171 122 32
NaeI GCC'GGC
Cuts at: 0 406 893 1515
Size: 406 487 622
Fragments arranged by size:
622 487 406
NarI GG'CG_CC
Cuts at: 0 1037 1515
Size: 1037 478
NcoI C'CATG_G
Cuts at: 0 59 1073 1515
Size: 59 1014 442
Fragments arranged by size:
1014 442 59
NdeI CA'TA_TG
Cuts at: 0 783 1515
Size: 783 732
NgoMIV G'CCGG_C
Cuts at: 0 404 891 1515
Size: 404 487 624
Fragments arranged by size:
624 487 404
NheI G'CTAG_C
Cuts at: 0 901 1515
Size: 901 614
NspI r_CATG'y
Cuts at: 0 354 656 1200 1515
Size: 354 302 544 315
Fragments arranged by size:
544 354 315 302
PasI CC'CwG_GG
Cuts at: 0 69 81 447 1095 1515
Size: 69 12 366 648 420
Fragments arranged by size:
648 420 366 69 12
PciI A'CATG_T
Cuts at: 0 1196 1515
Size: 1196 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 12 1515
Size: 12 1503
PfoI T'CCnGG_A
Cuts at: 0 1112 1160 1432 1515
Size: 1112 48 272 83
Fragments arranged by size:
1112 272 83 48
PmlI CAC'GTG
Cuts at: 0 95 1515
Size: 95 1420
PspOMI G'GGCC_C
Cuts at: 0 199 1515
Size: 199 1316
PstI C_TGCA'G
Cuts at: 0 1259 1478 1515
Size: 1259 219 37
Fragments arranged by size:
1259 219 37
PvuII CAG'CTG
Cuts at: 0 32 1222 1393 1515
Size: 32 1190 171 122
Fragments arranged by size:
1190 171 122 32
SexAI A'CCwGG_T
Cuts at: 0 956 1515
Size: 956 559
SfcI C'TryA_G
Cuts at: 0 1255 1474 1515
Size: 1255 219 41
Fragments arranged by size:
1255 219 41
SfoI GGC'GCC
Cuts at: 0 1038 1515
Size: 1038 477
SgrAI Cr'CCGG_yG
Cuts at: 0 404 1515
Size: 404 1111
SphI G_CATG'C
Cuts at: 0 656 1515
Size: 656 859
StuI AGG'CCT
Cuts at: 0 344 570 1515
Size: 344 226 945
Fragments arranged by size:
945 344 226
StyI C'CwwG_G
Cuts at: 0 59 1073 1515
Size: 59 1014 442
Fragments arranged by size:
1014 442 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 718 975 1225 1515
Size: 30 688 257 250 290
Fragments arranged by size:
688 290 257 250 30
TatI w'GTAC_w
Cuts at: 0 719 1515
Size: 719 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 755 787 1515
Size: 755 32 728
Fragments arranged by size:
755 728 32
Tth111I GACn'n_nGTC
Cuts at: 0 1066 1515
Size: 1066 449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 788 1515
Size: 276 512 727
Fragments arranged by size:
727 512 276
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FseI HaeII KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmlI PspOMI PstI
PvuII SexAI SfcI SfoI SgrAI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BglII BlpI BplI BpuEI
BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BssHII
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EcoICRI EcoRI EcoRV FalI FspI FspAI Hin4I HincII
HindIII HpaI MfeI MluI MmeI NotI NruI NsiI
PacI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI XmnI ZraI