Restriction map for
pFN21AE6444
(Linear) (Six-base) MAPSORT of: KIEE6444.seq Check: 4603 from: 1 to: 1530
KIEE6444.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1014 1530
Size: 1014 516
AfeI AGC'GCT
Cuts at: 0 239 1530
Size: 239 1291
AflIII A'CryG_T
Cuts at: 0 722 1530
Size: 722 808
AhdI GACnn_n'nnGTC
Cuts at: 0 1035 1525 1530
Size: 1035 490 5
Fragments arranged by size:
1035 490 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 525 1530
Size: 367 158 1005
Fragments arranged by size:
1005 367 158
AlwNI CAG_nnn'CTG
Cuts at: 0 492 660 1295 1393 1530
Size: 492 168 635 98 137
Fragments arranged by size:
635 492 168 137 98
AvaI C'yCGr_G
Cuts at: 0 203 1318 1530
Size: 203 1115 212
Fragments arranged by size:
1115 212 203
BanI G'GyrC_C
Cuts at: 0 83 223 438 630 1530
Size: 83 140 215 192 900
Fragments arranged by size:
900 215 192 140 83
BanII G_rGCy'C
Cuts at: 0 204 274 1319 1530
Size: 204 70 1045 211
Fragments arranged by size:
1045 211 204 70
BbeI G_GCGC'C
Cuts at: 0 227 1530
Size: 227 1303
BbsI GAAGACnn'nnnn_
Cuts at: 0 767 1016 1530
Size: 767 249 514
Fragments arranged by size:
767 514 249
BbvCI CC'TCA_GC
Cuts at: 0 518 737 1530
Size: 518 219 793
Fragments arranged by size:
793 518 219
Bme1580I G_kGCm'C
Cuts at: 0 88 1530
Size: 88 1442
BmgBI CAC'GTC
Cuts at: 0 114 1530
Size: 114 1416
BmrI ACTGGGnnnn_n'
Cuts at: 0 863 1530
Size: 863 667
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 817 981 1530
Size: 817 164 549
Fragments arranged by size:
817 549 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 676 1370 1530
Size: 121 555 694 160
Fragments arranged by size:
694 555 160 121
Bpu10I CC'TnA_GC
Cuts at: 0 518 737 1530
Size: 518 219 793
Fragments arranged by size:
793 518 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 951 1264 1530
Size: 787 164 313 266
Fragments arranged by size:
787 313 266 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 1530
Size: 97 1433
BsaAI yAC'GTr
Cuts at: 0 723 1530
Size: 723 807
BsaHI Gr'CG_yC
Cuts at: 0 224 1530
Size: 224 1306
BsaWI w'CCGG_w
Cuts at: 0 1193 1530
Size: 1193 337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 954 1174 1177 1252 1420 1423 1530
Size: 954 220 3 75 168 3 107
Fragments arranged by size:
954 220 168 107 75 3 3
BseYI C'CCAG_C
Cuts at: 0 129 528 535 1136 1530
Size: 129 399 7 601 394
Fragments arranged by size:
601 399 394 129 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 373 495 1530
Size: 229 144 122 1035
Fragments arranged by size:
1035 229 144 122
BsiHKAI G_wGCw'C
Cuts at: 0 338 926 1109 1530
Size: 338 588 183 421
Fragments arranged by size:
588 421 338 183
BsmI GAATG_Cn'
Cuts at: 0 1203 1530
Size: 1203 327
Bsp1286I G_dGCh'C
Cuts at: 0 88 204 274 338 926 1109 1319 1530
Size: 88 116 70 64 588 183 210 211
Fragments arranged by size:
588 211 210 183 116 88 70 64
BspEI T'CCGG_A
Cuts at: 0 1193 1530
Size: 1193 337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1014 1530
Size: 1014 516
BsrBI CCG'CTC
Cuts at: 0 599 1530
Size: 599 931
BsrDI GCAATG_nn'
Cuts at: 0 960 1500 1530
Size: 960 540 30
Fragments arranged by size:
960 540 30
BsrFI r'CCGG_y
Cuts at: 0 80 400 1530
Size: 80 320 1130
Fragments arranged by size:
1130 320 80
Bsu36I CC'TnA_GG
Cuts at: 0 35 1530
Size: 35 1495
BtgI C'CryG_G
Cuts at: 0 89 1346 1487 1530
Size: 89 1257 141 43
Fragments arranged by size:
1257 141 89 43
BtsI GCAGTG_nn'
Cuts at: 0 356 1530
Size: 356 1174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 754 789 1530
Size: 754 35 741
Fragments arranged by size:
754 741 35
DraIII CAC_nnn'GTG
Cuts at: 0 630 1530
Size: 630 900
DrdI GACnn_nn'nnGTC
Cuts at: 0 556 1530
Size: 556 974
EarI CTCTTCn'nnn_
Cuts at: 0 473 1307 1530
Size: 473 834 223
Fragments arranged by size:
834 473 223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 373 1530
Size: 373 1157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 676 1370 1530
Size: 121 555 694 160
Fragments arranged by size:
694 555 160 121
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 736 747 1530
Size: 691 45 11 783
Fragments arranged by size:
783 691 45 11
EcoO109I rG'GnC_Cy
Cuts at: 0 31 489 753 1469 1530
Size: 31 458 264 716 61
Fragments arranged by size:
716 458 264 61 31
FspI TGC'GCA
Cuts at: 0 343 1530
Size: 343 1187
HaeII r_GCGC'y
Cuts at: 0 227 241 1530
Size: 227 14 1289
Fragments arranged by size:
1289 227 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1511 1530
Size: 1021 32 458 19
Fragments arranged by size:
1021 458 32 19
KasI G'GCGC_C
Cuts at: 0 223 1530
Size: 223 1307
MspA1I CmG'CkG
Cuts at: 0 91 517 929 1530
Size: 91 426 412 601
Fragments arranged by size:
601 426 412 91
NaeI GCC'GGC
Cuts at: 0 402 1530
Size: 402 1128
NarI GG'CG_CC
Cuts at: 0 224 1530
Size: 224 1306
NgoMIV G'CCGG_C
Cuts at: 0 400 1530
Size: 400 1130
PasI CC'CwG_GG
Cuts at: 0 12 1530
Size: 12 1518
PflMI CCAn_nnn'nTGG
Cuts at: 0 1393 1530
Size: 1393 137
PfoI T'CCnGG_A
Cuts at: 0 692 1530
Size: 692 838
PmlI CAC'GTG
Cuts at: 0 723 1530
Size: 723 807
PpuMI rG'GwC_Cy
Cuts at: 0 1469 1530
Size: 1469 61
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 224 256 1530
Size: 224 32 1274
Fragments arranged by size:
1274 224 32
PvuII CAG'CTG
Cuts at: 0 517 1530
Size: 517 1013
SacII CC_GC'GG
Cuts at: 0 92 1530
Size: 92 1438
SanDI GG'GwC_CC
Cuts at: 0 1469 1530
Size: 1469 61
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 1530
Size: 1307 223
SexAI A'CCwGG_T
Cuts at: 0 1523 1530
Size: 1523 7
SfoI GGC'GCC
Cuts at: 0 225 1530
Size: 225 1305
SmlI C'TyrA_G
Cuts at: 0 802 966 1279 1530
Size: 802 164 313 251
Fragments arranged by size:
802 313 251 164
StuI AGG'CCT
Cuts at: 0 40 742 1530
Size: 40 702 788
Fragments arranged by size:
788 702 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 724 1048 1530
Size: 724 324 482
Fragments arranged by size:
724 482 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1012 1530
Size: 1012 518
Enzymes that do cut:
AarI AfeI AflIII AhdI AlfI AlwNI AvaI BanI
BanII BbeI BbsI BbvCI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI Bsu36I BtgI BtsI CspCI DraIII DrdI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI
PmlI PpuMI PsrI PvuII SacII SanDI SapI SexAI
SfoI SmlI StuI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BsaBI BsaXI BsiEI BsiWI BsmBI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI DraI EaeI EagI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI