Restriction map for
pFN21AE6443
(Linear) (Six-base) MAPSORT of: KIEE6443.seq Check: 3604 from: 1 to: 1347
KIEE6443.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 958 1347
Size: 958 389
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1159 1347
Size: 914 245 188
Fragments arranged by size:
914 245 188
AhdI GACnn_n'nnGTC
Cuts at: 0 1073 1347
Size: 1073 274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 966 1347
Size: 966 381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 1347
Size: 740 32 575
Fragments arranged by size:
740 575 32
AlwNI CAG_nnn'CTG
Cuts at: 0 937 1347
Size: 937 410
ApaI G_GGCC'C
Cuts at: 0 35 851 1347
Size: 35 816 496
Fragments arranged by size:
816 496 35
AvaI C'yCGr_G
Cuts at: 0 208 453 526 543 720 1347
Size: 208 245 73 17 177 627
Fragments arranged by size:
627 245 208 177 73 17
BanI G'GyrC_C
Cuts at: 0 976 1129 1347
Size: 976 153 218
Fragments arranged by size:
976 218 153
BanII G_rGCy'C
Cuts at: 0 35 727 851 1347
Size: 35 692 124 496
Fragments arranged by size:
692 496 124 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 282 1347
Size: 234 48 1065
Fragments arranged by size:
1065 234 48
BglI GCCn_nnn'nGGC
Cuts at: 0 522 974 1347
Size: 522 452 373
Fragments arranged by size:
522 452 373
BglII A'GATC_T
Cuts at: 0 771 1347
Size: 771 576
Bme1580I G_kGCm'C
Cuts at: 0 35 409 460 829 851 1168 1312 1347
Size: 35 374 51 369 22 317 144 35
Fragments arranged by size:
374 369 317 144 51 35 35 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 304 508 640 1118 1223 1347
Size: 271 33 204 132 478 105 124
Fragments arranged by size:
478 271 204 132 124 105 33
Bpu10I CC'TnA_GC
Cuts at: 0 1294 1347
Size: 1294 53
BsaAI yAC'GTr
Cuts at: 0 141 1149 1347
Size: 141 1008 198
Fragments arranged by size:
1008 198 141
BsaWI w'CCGG_w
Cuts at: 0 906 1347
Size: 906 441
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 104 134 1347
Size: 104 30 1213
Fragments arranged by size:
1213 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 723 742 1347
Size: 28 695 19 605
Fragments arranged by size:
695 605 28 19
BseYI C'CCAG_C
Cuts at: 0 295 693 1347
Size: 295 398 654
Fragments arranged by size:
654 398 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1347
Size: 241 1106
BsiHKAI G_wGCw'C
Cuts at: 0 467 1347
Size: 467 880
BsmBI CGTCTCn'nnnn_
Cuts at: 0 771 1347
Size: 771 576
Bsp1286I G_dGCh'C
Cuts at: 0 35 409 460 467 727 829 851 1168
Size: 35 374 51 7 260 102 22 317
Cuts at: 1168 1312 1347
Size: 144 35
Fragments arranged by size:
374 317 260 144 102 51 35 35
22 7
BspEI T'CCGG_A
Cuts at: 0 906 1347
Size: 906 441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 958 1118 1347
Size: 958 160 229
Fragments arranged by size:
958 229 160
BsrFI r'CCGG_y
Cuts at: 0 1262 1347
Size: 1262 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1163 1347
Size: 1163 184
BstEII G'GTnAC_C
Cuts at: 0 378 1347
Size: 378 969
BstYI r'GATC_y
Cuts at: 0 771 1280 1347
Size: 771 509 67
Fragments arranged by size:
771 509 67
Bsu36I CC'TnA_GG
Cuts at: 0 385 1347
Size: 385 962
BtgI C'CryG_G
Cuts at: 0 820 1034 1347
Size: 820 214 313
Fragments arranged by size:
820 313 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 555 576 1347
Size: 555 21 771
Fragments arranged by size:
771 555 21
BtsI GCAGTG_nn'
Cuts at: 0 41 827 967 1168 1347
Size: 41 786 140 201 179
Fragments arranged by size:
786 201 179 140 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 1029 1347
Size: 1029 318
EaeI y'GGCC_r
Cuts at: 0 312 1347
Size: 312 1035
EarI CTCTTCn'nnn_
Cuts at: 0 1208 1347
Size: 1208 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 304 508 640 914 1118 1159 1223
Size: 271 33 204 132 274 204 41 64
Cuts at: 1223 1347
Size: 124
Fragments arranged by size:
274 271 204 204 132 124 64 41
33
EcoO109I rG'GnC_Cy
Cuts at: 0 31 32 338 847 1056 1225 1347
Size: 31 1 306 509 209 169 122
Fragments arranged by size:
509 306 209 169 122 31 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 781 813 1059 1091 1214 1246 1347
Size: 781 32 246 32 123 32 101
Fragments arranged by size:
781 246 123 101 32 32 32
MspA1I CmG'CkG
Cuts at: 0 318 440 554 787 822 1347
Size: 318 122 114 233 35 525
Fragments arranged by size:
525 318 233 122 114 35
NcoI C'CATG_G
Cuts at: 0 1034 1347
Size: 1034 313
PasI CC'CwG_GG
Cuts at: 0 69 498 1347
Size: 69 429 849
Fragments arranged by size:
849 429 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 1126 1347
Size: 1126 221
PfoI T'CCnGG_A
Cuts at: 0 685 1347
Size: 685 662
PpuMI rG'GwC_Cy
Cuts at: 0 338 1347
Size: 338 1009
PsiI TTA'TAA
Cuts at: 0 1091 1347
Size: 1091 256
PspOMI G'GGCC_C
Cuts at: 0 31 847 1347
Size: 31 816 500
Fragments arranged by size:
816 500 31
PstI C_TGCA'G
Cuts at: 0 12 1347
Size: 12 1335
PvuII CAG'CTG
Cuts at: 0 554 1347
Size: 554 793
SacII CC_GC'GG
Cuts at: 0 823 1347
Size: 823 524
SexAI A'CCwGG_T
Cuts at: 0 374 584 1347
Size: 374 210 763
Fragments arranged by size:
763 374 210
SfcI C'TryA_G
Cuts at: 0 8 1347
Size: 8 1339
SmaI CCC'GGG
Cuts at: 0 545 722 1347
Size: 545 177 625
Fragments arranged by size:
625 545 177
StyI C'CwwG_G
Cuts at: 0 1034 1347
Size: 1034 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 960 1137 1347
Size: 639 321 177 210
Fragments arranged by size:
639 321 210 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 104 136 1347
Size: 104 32 1211
Fragments arranged by size:
1211 104 32
Tth111I GACn'n_nGTC
Cuts at: 0 222 586 649 715 1347
Size: 222 364 63 66 632
Fragments arranged by size:
632 364 222 66 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1008 1347
Size: 1008 339
XmaI C'CCGG_G
Cuts at: 0 543 720 1347
Size: 543 177 627
Fragments arranged by size:
627 543 177
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI AvaI
BanI BanII BbsI BglI BglII Bme1580I BpmI Bpu10I
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrFI BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I
Hin4I MspA1I NcoI PasI PflMI PfoI PpuMI PsiI
PspOMI PstI PvuII SacII SexAI SfcI SmaI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI
BsiEI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XbaI XhoI XmnI ZraI