Restriction map for
pFN21AE6441
(Linear) (Six-base) MAPSORT of: KIEE6441.seq Check: 2127 from: 1 to: 1392
KIEE6441.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 641 1392
Size: 641 751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 310 1392
Size: 256 54 1082
Fragments arranged by size:
1082 256 54
AfeI AGC'GCT
Cuts at: 0 73 1392
Size: 73 1319
AflIII A'CryG_T
Cuts at: 0 811 1100 1142 1392
Size: 811 289 42 250
Fragments arranged by size:
811 289 250 42
AlwNI CAG_nnn'CTG
Cuts at: 0 748 1392
Size: 748 644
ApaI G_GGCC'C
Cuts at: 0 293 738 1392
Size: 293 445 654
Fragments arranged by size:
654 445 293
ApoI r'AATT_y
Cuts at: 0 245 1392
Size: 245 1147
AvaI C'yCGr_G
Cuts at: 0 293 1392
Size: 293 1099
BanI G'GyrC_C
Cuts at: 0 77 370 557 1392
Size: 77 293 187 835
Fragments arranged by size:
835 293 187 77
BanII G_rGCy'C
Cuts at: 0 230 293 738 1392
Size: 230 63 445 654
Fragments arranged by size:
654 445 230 63
BbeI G_GCGC'C
Cuts at: 0 81 561 1392
Size: 81 480 831
Fragments arranged by size:
831 480 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 875 1392
Size: 608 267 517
Fragments arranged by size:
608 517 267
BlpI GC'TnA_GC
Cuts at: 0 1253 1392
Size: 1253 139
Bme1580I G_kGCm'C
Cuts at: 0 293 738 1392
Size: 293 445 654
Fragments arranged by size:
654 445 293
BmgBI CAC'GTC
Cuts at: 0 126 1103 1392
Size: 126 977 289
Fragments arranged by size:
977 289 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 751 1392
Size: 751 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1063 1092 1392
Size: 370 693 29 300
Fragments arranged by size:
693 370 300 29
BsaHI Gr'CG_yC
Cuts at: 0 78 558 1392
Size: 78 480 834
Fragments arranged by size:
834 480 78
BsaWI w'CCGG_w
Cuts at: 0 1310 1392
Size: 1310 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 302 332 1090 1120 1392
Size: 302 30 758 30 272
Fragments arranged by size:
758 302 272 30 30
BseYI C'CCAG_C
Cuts at: 0 57 117 708 793 1355 1392
Size: 57 60 591 85 562 37
Fragments arranged by size:
591 562 85 60 57 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1081 1381 1392
Size: 1014 67 300 11
Fragments arranged by size:
1014 300 67 11
BsiEI CG_ry'CG
Cuts at: 0 772 1392
Size: 772 620
BsiHKAI G_wGCw'C
Cuts at: 0 230 1373 1392
Size: 230 1143 19
Fragments arranged by size:
1143 230 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 820 1392
Size: 820 572
Bsp1286I G_dGCh'C
Cuts at: 0 230 293 738 1373 1392
Size: 230 63 445 635 19
Fragments arranged by size:
635 445 230 63 19
BspEI T'CCGG_A
Cuts at: 0 1310 1392
Size: 1310 82
BspHI T'CATG_A
Cuts at: 0 701 1392
Size: 701 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 692 1130 1328 1392
Size: 692 438 198 64
Fragments arranged by size:
692 438 198 64
BsrBI CCG'CTC
Cuts at: 0 740 768 786 825 1392
Size: 740 28 18 39 567
Fragments arranged by size:
740 567 39 28 18
BsrDI GCAATG_nn'
Cuts at: 0 410 1392
Size: 410 982
BsrFI r'CCGG_y
Cuts at: 0 692 1392
Size: 692 700
BssSI C'ACGA_G
Cuts at: 0 849 1392
Size: 849 543
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1368 1392
Size: 1368 24
BstEII G'GTnAC_C
Cuts at: 0 186 1392
Size: 186 1206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 1116 1134 1392
Size: 885 231 18 258
Fragments arranged by size:
885 258 231 18
Bsu36I CC'TnA_GG
Cuts at: 0 749 1392
Size: 749 643
DraI TTT'AAA
Cuts at: 0 666 1392
Size: 666 726
DrdI GACnn_nn'nnGTC
Cuts at: 0 1087 1392
Size: 1087 305
EaeI y'GGCC_r
Cuts at: 0 167 530 826 1392
Size: 167 363 296 566
Fragments arranged by size:
566 363 296 167
EarI CTCTTCn'nnn_
Cuts at: 0 703 1392
Size: 703 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 625 1093 1392
Size: 625 468 299
Fragments arranged by size:
625 468 299
EcoICRI GAG'CTC
Cuts at: 0 228 1392
Size: 228 1164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 310 370 1063 1092 1392
Size: 256 54 60 693 29 300
Fragments arranged by size:
693 300 256 60 54 29
EcoO109I rG'GnC_Cy
Cuts at: 0 290 734 753 967 1392
Size: 290 444 19 214 425
Fragments arranged by size:
444 425 290 214 19
EcoRI G'AATT_C
Cuts at: 0 245 1392
Size: 245 1147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 340 1392
Size: 340 1052
HaeII r_GCGC'y
Cuts at: 0 31 75 81 561 1392
Size: 31 44 6 480 831
Fragments arranged by size:
831 480 44 31 6
HincII GTy'rAC
Cuts at: 0 263 1392
Size: 263 1129
KasI G'GCGC_C
Cuts at: 0 77 557 1392
Size: 77 480 835
Fragments arranged by size:
835 480 77
MluI A'CGCG_T
Cuts at: 0 811 1392
Size: 811 581
MscI TGG'CCA
Cuts at: 0 169 532 1392
Size: 169 363 860
Fragments arranged by size:
860 363 169
MslI CAynn'nnrTG
Cuts at: 0 473 1132 1392
Size: 473 659 260
Fragments arranged by size:
659 473 260
MspA1I CmG'CkG
Cuts at: 0 48 1257 1392
Size: 48 1209 135
Fragments arranged by size:
1209 135 48
NarI GG'CG_CC
Cuts at: 0 78 558 1392
Size: 78 480 834
Fragments arranged by size:
834 480 78
NspI r_CATG'y
Cuts at: 0 103 720 1146 1392
Size: 103 617 426 246
Fragments arranged by size:
617 426 246 103
PciI A'CATG_T
Cuts at: 0 1142 1392
Size: 1142 250
PpuMI rG'GwC_Cy
Cuts at: 0 753 1392
Size: 753 639
PspOMI G'GGCC_C
Cuts at: 0 289 734 1392
Size: 289 445 658
Fragments arranged by size:
658 445 289
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 532 564 1392
Size: 532 32 828
Fragments arranged by size:
828 532 32
PstI C_TGCA'G
Cuts at: 0 689 1262 1392
Size: 689 573 130
Fragments arranged by size:
689 573 130
PvuII CAG'CTG
Cuts at: 0 1257 1392
Size: 1257 135
SacI G_AGCT'C
Cuts at: 0 230 1392
Size: 230 1162
SapI GCTCTTCn'nnn_
Cuts at: 0 703 1392
Size: 703 689
SfcI C'TryA_G
Cuts at: 0 685 1258 1392
Size: 685 573 134
Fragments arranged by size:
685 573 134
SfoI GGC'GCC
Cuts at: 0 79 559 1392
Size: 79 480 833
Fragments arranged by size:
833 480 79
SphI G_CATG'C
Cuts at: 0 720 1392
Size: 720 672
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1075 1347 1392
Size: 1075 272 45
Fragments arranged by size:
1075 272 45
TatI w'GTAC_w
Cuts at: 0 321 335 1392
Size: 321 14 1057
Fragments arranged by size:
1057 321 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1392
Size: 1090 32 270
Fragments arranged by size:
1090 270 32
Tth111I GACn'n_nGTC
Cuts at: 0 757 1392
Size: 757 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 1134 1392
Size: 492 642 258
Fragments arranged by size:
642 492 258
XmnI GAAnn'nnTTC
Cuts at: 0 1009 1392
Size: 1009 383
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BlpI Bme1580I BmgBI BmrI
BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI Bsu36I DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
HincII KasI MluI MscI MslI MspA1I NarI NspI
PciI PpuMI PspOMI PsrI PstI PvuII SacI SapI
SfcI SfoI SphI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI
BsiWI BsmI BsrGI BssHII BstBI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII EagI EcoNI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI XbaI
XhoI XmaI ZraI