Restriction map for
pFN21AE6440
(Linear) (Six-base) MAPSORT of: KIEE6440.seq Check: 8079 from: 1 to: 1725
KIEE6440.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 253 1226 1725
Size: 253 973 499
Fragments arranged by size:
973 499 253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1060 1219 1403 1725
Size: 40 1020 159 184 322
Fragments arranged by size:
1020 322 184 159 40
AhdI GACnn_n'nnGTC
Cuts at: 0 427 1725
Size: 427 1298
AleI CACnn'nnGTG
Cuts at: 0 119 1725
Size: 119 1606
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 1725
Size: 232 1493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1663 1695 1725
Size: 1663 32 30
Fragments arranged by size:
1663 32 30
AlwNI CAG_nnn'CTG
Cuts at: 0 1197 1373 1725
Size: 1197 176 352
Fragments arranged by size:
1197 352 176
ApoI r'AATT_y
Cuts at: 0 400 739 1045 1209 1474 1725
Size: 400 339 306 164 265 251
Fragments arranged by size:
400 339 306 265 251 164
BanI G'GyrC_C
Cuts at: 0 30 1725
Size: 30 1695
BanII G_rGCy'C
Cuts at: 0 798 1574 1725
Size: 798 776 151
Fragments arranged by size:
798 776 151
BbeI G_GCGC'C
Cuts at: 0 34 1725
Size: 34 1691
BbsI GAAGACnn'nnnn_
Cuts at: 0 1382 1725
Size: 1382 343
BbvCI CC'TCA_GC
Cuts at: 0 63 1725
Size: 63 1662
BglII A'GATC_T
Cuts at: 0 500 1725
Size: 500 1225
BlpI GC'TnA_GC
Cuts at: 0 292 1725
Size: 292 1433
Bme1580I G_kGCm'C
Cuts at: 0 116 1724 1725
Size: 116 1608 1
Fragments arranged by size:
1608 116 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 672 1138 1725
Size: 672 466 587
Fragments arranged by size:
672 587 466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1455 1725
Size: 1455 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 352 1345 1630 1725
Size: 56 296 993 285 95
Fragments arranged by size:
993 296 285 95 56
Bpu10I CC'TnA_GC
Cuts at: 0 63 1725
Size: 63 1662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1725
Size: 149 1576
BsaI GGTCTCn'nnnn_
Cuts at: 0 1703 1725
Size: 1703 22
BsaBI GATnn'nnATC
Cuts at: 0 671 1725
Size: 671 1054
BsaHI Gr'CG_yC
Cuts at: 0 31 1725
Size: 31 1694
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 600 630 1663 1693 1725
Size: 190 30 380 30 1033 30 32
Fragments arranged by size:
1033 380 190 32 30 30 30
BseYI C'CCAG_C
Cuts at: 0 97 777 790 1149 1725
Size: 97 680 13 359 576
Fragments arranged by size:
680 576 359 97 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1660 1725
Size: 228 1432 65
Fragments arranged by size:
1432 228 65
BsmI GAATG_Cn'
Cuts at: 0 312 1725
Size: 312 1413
Bsp1286I G_dGCh'C
Cuts at: 0 116 798 1574 1724 1725
Size: 116 682 776 150 1
Fragments arranged by size:
776 682 150 116 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 253 1226 1725
Size: 224 29 973 499
Fragments arranged by size:
973 499 224 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 904 1599 1725
Size: 904 695 126
Fragments arranged by size:
904 695 126
BstYI r'GATC_y
Cuts at: 0 500 1725
Size: 500 1225
BtgI C'CryG_G
Cuts at: 0 1377 1574 1725
Size: 1377 197 151
Fragments arranged by size:
1377 197 151
BtsI GCAGTG_nn'
Cuts at: 0 583 1134 1725
Size: 583 551 591
Fragments arranged by size:
591 583 551
EaeI y'GGCC_r
Cuts at: 0 354 494 1305 1725
Size: 354 140 811 420
Fragments arranged by size:
811 420 354 140
EarI CTCTTCn'nnn_
Cuts at: 0 59 386 1129 1534 1725
Size: 59 327 743 405 191
Fragments arranged by size:
743 405 327 191 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1562 1633 1725
Size: 1562 71 92
Fragments arranged by size:
1562 92 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 56 352 1060 1219 1345 1403 1630
Size: 40 16 296 708 159 126 58 227
Cuts at: 1630 1725
Size: 95
Fragments arranged by size:
708 296 227 159 126 95 58 40
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1666 1725
Size: 1666 59
EcoRV GAT'ATC
Cuts at: 0 1294 1725
Size: 1294 431
FspI TGC'GCA
Cuts at: 0 1334 1725
Size: 1334 391
HaeII r_GCGC'y
Cuts at: 0 34 1725
Size: 34 1691
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 263 295 413 445 1725
Size: 73 32 158 32 118 32 1280
Fragments arranged by size:
1280 158 118 73 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1056 1725
Size: 1056 669
KasI G'GCGC_C
Cuts at: 0 30 1725
Size: 30 1695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1058 1205 1418 1466 1725
Size: 915 143 147 213 48 259
Fragments arranged by size:
915 259 213 147 143 48
MscI TGG'CCA
Cuts at: 0 356 496 1725
Size: 356 140 1229
Fragments arranged by size:
1229 356 140
MslI CAynn'nnrTG
Cuts at: 0 119 1725
Size: 119 1606
MspA1I CmG'CkG
Cuts at: 0 790 1725
Size: 790 935
NarI GG'CG_CC
Cuts at: 0 31 1725
Size: 31 1694
NdeI CA'TA_TG
Cuts at: 0 729 1725
Size: 729 996
PasI CC'CwG_GG
Cuts at: 0 761 1725
Size: 761 964
PflMI CCAn_nnn'nTGG
Cuts at: 0 718 1662 1725
Size: 718 944 63
Fragments arranged by size:
944 718 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1663 1695 1725
Size: 1663 32 30
Fragments arranged by size:
1663 32 30
PstI C_TGCA'G
Cuts at: 0 1129 1725
Size: 1129 596
PvuII CAG'CTG
Cuts at: 0 790 1725
Size: 790 935
SfcI C'TryA_G
Cuts at: 0 409 911 1125 1725
Size: 409 502 214 600
Fragments arranged by size:
600 502 409 214
SfoI GGC'GCC
Cuts at: 0 32 1725
Size: 32 1693
SmlI C'TyrA_G
Cuts at: 0 164 1725
Size: 164 1561
SspI AAT'ATT
Cuts at: 0 454 1008 1725
Size: 454 554 717
Fragments arranged by size:
717 554 454
StuI AGG'CCT
Cuts at: 0 190 1725
Size: 190 1535
StyI C'CwwG_G
Cuts at: 0 386 1713 1725
Size: 386 1327 12
Fragments arranged by size:
1327 386 12
TatI w'GTAC_w
Cuts at: 0 1340 1725
Size: 1340 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 1725
Size: 190 32 1503
Fragments arranged by size:
1503 190 32
Tth111I GACn'n_nGTC
Cuts at: 0 1144 1725
Size: 1144 581
XbaI T'CTAG_A
Cuts at: 0 457 1063 1725
Size: 457 606 662
Fragments arranged by size:
662 606 457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1222 1725
Size: 1222 503
XmnI GAAnn'nnTTC
Cuts at: 0 465 739 850 1678 1725
Size: 465 274 111 828 47
Fragments arranged by size:
828 465 274 111 47
Enzymes that do cut:
AarI AcuI AhdI AleI AlfI AloI AlwNI ApoI
BanI BanII BbeI BbsI BbvCI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseYI BsgI BsmI Bsp1286I BspMI BstXI BstYI
BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoRV
FspI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NarI NdeI PasI PflMI PpiI PstI PvuII
SfcI SfoI SmlI SspI StuI StyI TatI TstI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI BmgBI BmtI
BsaAI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoO109I EcoRI FalI FseI
FspAI HincII HpaI KpnI MfeI MluI NaeI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII XhoI XmaI ZraI