Restriction map for
pFN21AE6439
(Linear) (Six-base) MAPSORT of: KIEE6439.seq Check: 2177 from: 1 to: 1428
KIEE6439.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 1428
Size: 459 969
AfeI AGC'GCT
Cuts at: 0 1192 1428
Size: 1192 236
AlwNI CAG_nnn'CTG
Cuts at: 0 1365 1428
Size: 1365 63
ApaLI G'TGCA_C
Cuts at: 0 542 957 1428
Size: 542 415 471
Fragments arranged by size:
542 471 415
ApoI r'AATT_y
Cuts at: 0 751 991 1428
Size: 751 240 437
Fragments arranged by size:
751 437 240
AseI AT'TA_AT
Cuts at: 0 1385 1428
Size: 1385 43
BamHI G'GATC_C
Cuts at: 0 1219 1413 1428
Size: 1219 194 15
Fragments arranged by size:
1219 194 15
BanI G'GyrC_C
Cuts at: 0 7 1428
Size: 7 1421
BfrBI ATG'CAT
Cuts at: 0 821 1428
Size: 821 607
BglII A'GATC_T
Cuts at: 0 1259 1428
Size: 1259 169
Bme1580I G_kGCm'C
Cuts at: 0 469 546 961 1428
Size: 469 77 415 467
Fragments arranged by size:
469 467 415 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 301 611 1428
Size: 301 310 817
Fragments arranged by size:
817 310 301
BsaAI yAC'GTr
Cuts at: 0 1029 1428
Size: 1029 399
BseYI C'CCAG_C
Cuts at: 0 151 279 1428
Size: 151 128 1149
Fragments arranged by size:
1149 151 128
BsiHKAI G_wGCw'C
Cuts at: 0 546 961 1428
Size: 546 415 467
Fragments arranged by size:
546 467 415
BsmI GAATG_Cn'
Cuts at: 0 823 1428
Size: 823 605
Bsp1286I G_dGCh'C
Cuts at: 0 469 546 961 1428
Size: 469 77 415 467
Fragments arranged by size:
469 467 415 77
BspHI T'CATG_A
Cuts at: 0 1121 1428
Size: 1121 307
BsrDI GCAATG_nn'
Cuts at: 0 635 1428
Size: 635 793
BsrFI r'CCGG_y
Cuts at: 0 223 1428
Size: 223 1205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1029 1428
Size: 1029 399
BstYI r'GATC_y
Cuts at: 0 158 1219 1259 1413 1428
Size: 158 1061 40 154 15
Fragments arranged by size:
1061 158 154 40 15
Bsu36I CC'TnA_GG
Cuts at: 0 104 968 1428
Size: 104 864 460
Fragments arranged by size:
864 460 104
BtgI C'CryG_G
Cuts at: 0 495 1105 1428
Size: 495 610 323
Fragments arranged by size:
610 495 323
BtsI GCAGTG_nn'
Cuts at: 0 814 1428
Size: 814 614
ClaI AT'CG_AT
Cuts at: 0 1389 1428
Size: 1389 39
DraI TTT'AAA
Cuts at: 0 1266 1428
Size: 1266 162
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1428
Size: 595 833
EaeI y'GGCC_r
Cuts at: 0 1152 1428
Size: 1152 276
EarI CTCTTCn'nnn_
Cuts at: 0 165 208 268 1428
Size: 165 43 60 1160
Fragments arranged by size:
1160 165 60 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 1428
Size: 459 969
EcoNI CCTnn'n_nnAGG
Cuts at: 0 197 1428
Size: 197 1231
EcoRI G'AATT_C
Cuts at: 0 751 1428
Size: 751 677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 920 1428
Size: 920 508
HaeII r_GCGC'y
Cuts at: 0 1194 1428
Size: 1194 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 491 523 712 744 1007 1039 1428
Size: 491 32 189 32 263 32 389
Fragments arranged by size:
491 389 263 189 32 32 32
HincII GTy'rAC
Cuts at: 0 1112 1428
Size: 1112 316
HpaI GTT'AAC
Cuts at: 0 1112 1428
Size: 1112 316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1404 1428
Size: 755 649 24
Fragments arranged by size:
755 649 24
MscI TGG'CCA
Cuts at: 0 1154 1428
Size: 1154 274
MslI CAynn'nnrTG
Cuts at: 0 500 1027 1168 1428
Size: 500 527 141 260
Fragments arranged by size:
527 500 260 141
MspA1I CmG'CkG
Cuts at: 0 1177 1428
Size: 1177 251
NaeI GCC'GGC
Cuts at: 0 225 1428
Size: 225 1203
NcoI C'CATG_G
Cuts at: 0 1105 1428
Size: 1105 323
NgoMIV G'CCGG_C
Cuts at: 0 223 1428
Size: 223 1205
NsiI A_TGCA'T
Cuts at: 0 823 1428
Size: 823 605
PmlI CAC'GTG
Cuts at: 0 1029 1428
Size: 1029 399
PsiI TTA'TAA
Cuts at: 0 1133 1428
Size: 1133 295
PvuII CAG'CTG
Cuts at: 0 1177 1428
Size: 1177 251
ScaI AGT'ACT
Cuts at: 0 796 1072 1428
Size: 796 276 356
Fragments arranged by size:
796 356 276
SfcI C'TryA_G
Cuts at: 0 322 1058 1428
Size: 322 736 370
Fragments arranged by size:
736 370 322
StuI AGG'CCT
Cuts at: 0 856 1428
Size: 856 572
StyI C'CwwG_G
Cuts at: 0 1105 1428
Size: 1105 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1294 1428
Size: 1294 134
TatI w'GTAC_w
Cuts at: 0 794 1070 1089 1428
Size: 794 276 19 339
Fragments arranged by size:
794 339 276 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 347 379 632 664 1428
Size: 347 32 253 32 764
Fragments arranged by size:
764 347 253 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 314 1428
Size: 314 1114
XmnI GAAnn'nnTTC
Cuts at: 0 90 1428
Size: 90 1338
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI ApoI AseI BamHI BanI
BfrBI BglII Bme1580I BmrI BsaAI BseYI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI DrdI EaeI EarI Eco57MI EcoNI
EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI PmlI PsiI
PvuII ScaI SfcI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraIII
EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI
HindIII KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI