Restriction map for
pFN21AE6438
(Linear) (Six-base) MAPSORT of: KIEE6438.seq Check: 6409 from: 1 to: 1398
KIEE6438.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 250 588 841 1010 1398
Size: 121 129 338 253 169 388
Fragments arranged by size:
388 338 253 169 129 121
AflII C'TTAA_G
Cuts at: 0 1269 1398
Size: 1269 129
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 532 564 1398
Size: 532 32 834
Fragments arranged by size:
834 532 32
AlwNI CAG_nnn'CTG
Cuts at: 0 56 1398
Size: 56 1342
ApoI r'AATT_y
Cuts at: 0 377 573 930 949 1398
Size: 377 196 357 19 449
Fragments arranged by size:
449 377 357 196 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 594 998 1398
Size: 594 404 400
Fragments arranged by size:
594 404 400
BciVI GTATCCnnnnn_n'
Cuts at: 0 1065 1398
Size: 1065 333
BglII A'GATC_T
Cuts at: 0 486 720 1398
Size: 486 234 678
Fragments arranged by size:
678 486 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1066 1398
Size: 313 753 332
Fragments arranged by size:
753 332 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1285 1398
Size: 745 540 113
Fragments arranged by size:
745 540 113
BsaAI yAC'GTr
Cuts at: 0 1350 1398
Size: 1350 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 1398
Size: 532 30 836
Fragments arranged by size:
836 532 30
BseYI C'CCAG_C
Cuts at: 0 1148 1398
Size: 1148 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1398
Size: 1000 398
BsiHKAI G_wGCw'C
Cuts at: 0 226 1398
Size: 226 1172
BsmI GAATG_Cn'
Cuts at: 0 349 1274 1398
Size: 349 925 124
Fragments arranged by size:
925 349 124
Bsp1286I G_dGCh'C
Cuts at: 0 226 1398
Size: 226 1172
BspHI T'CATG_A
Cuts at: 0 182 954 1224 1398
Size: 182 772 270 174
Fragments arranged by size:
772 270 182 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 397 1017 1398
Size: 338 59 620 381
Fragments arranged by size:
620 381 338 59
BsrDI GCAATG_nn'
Cuts at: 0 736 1398
Size: 736 662
BstAPI GCAn_nnn'nTGC
Cuts at: 0 353 1398
Size: 353 1045
BstYI r'GATC_y
Cuts at: 0 486 720 1304 1398
Size: 486 234 584 94
Fragments arranged by size:
584 486 234 94
BtgI C'CryG_G
Cuts at: 0 864 1398
Size: 864 534
BtsI GCAGTG_nn'
Cuts at: 0 54 1398
Size: 54 1344
DraI TTT'AAA
Cuts at: 0 1389 1398
Size: 1389 9
EaeI y'GGCC_r
Cuts at: 0 1071 1398
Size: 1071 327
EarI CTCTTCn'nnn_
Cuts at: 0 97 134 1398
Size: 97 37 1264
Fragments arranged by size:
1264 97 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 250 313 588 841 1010 1066 1398
Size: 121 129 63 275 253 169 56 332
Fragments arranged by size:
332 275 253 169 129 121 63 56
EcoO109I rG'GnC_Cy
Cuts at: 0 258 1173 1398
Size: 258 915 225
Fragments arranged by size:
915 258 225
EcoRI G'AATT_C
Cuts at: 0 573 1398
Size: 573 825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 739 1398
Size: 739 659
FspI TGC'GCA
Cuts at: 0 60 1398
Size: 60 1338
HincII GTy'rAC
Cuts at: 0 560 1398
Size: 560 838
MfeI C'AATT_G
Cuts at: 0 526 1398
Size: 526 872
MscI TGG'CCA
Cuts at: 0 1073 1398
Size: 1073 325
MspA1I CmG'CkG
Cuts at: 0 759 1398
Size: 759 639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 1398
Size: 532 32 834
Fragments arranged by size:
834 532 32
PvuII CAG'CTG
Cuts at: 0 759 1398
Size: 759 639
SfcI C'TryA_G
Cuts at: 0 1366 1398
Size: 1366 32
SmlI C'TyrA_G
Cuts at: 0 724 1269 1300 1398
Size: 724 545 31 98
Fragments arranged by size:
724 545 98 31
SnaBI TAC'GTA
Cuts at: 0 1350 1398
Size: 1350 48
SspI AAT'ATT
Cuts at: 0 1236 1398
Size: 1236 162
StuI AGG'CCT
Cuts at: 0 872 1398
Size: 872 526
StyI C'CwwG_G
Cuts at: 0 1216 1398
Size: 1216 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 1140 1398
Size: 78 1062 258
Fragments arranged by size:
1062 258 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1289 1321 1398
Size: 1289 32 77
Fragments arranged by size:
1289 77 32
XmnI GAAnn'nnTTC
Cuts at: 0 1236 1398
Size: 1236 162
Enzymes that do cut:
AcuI AflII AloI AlwNI ApoI BbsI BciVI BglII
BpmI BpuEI BsaAI BsaXI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BstAPI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI FspI
HincII MfeI MscI MspA1I PpiI PvuII SfcI SmlI
SnaBI SspI StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI