Restriction map for
pFN21AE6437
(Linear) (Six-base) MAPSORT of: KIEE6437.seq Check: 7725 from: 1 to: 1674
KIEE6437.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 1674
Size: 94 1580
Acc65I G'GTAC_C
Cuts at: 0 649 1674
Size: 649 1025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1674
Size: 656 1018
AflIII A'CryG_T
Cuts at: 0 555 1674
Size: 555 1119
AhdI GACnn_n'nnGTC
Cuts at: 0 1641 1674
Size: 1641 33
AlwNI CAG_nnn'CTG
Cuts at: 0 510 1674
Size: 510 1164
ApaI G_GGCC'C
Cuts at: 0 1426 1674
Size: 1426 248
ApoI r'AATT_y
Cuts at: 0 256 277 485 838 910 1279 1674
Size: 256 21 208 353 72 369 395
Fragments arranged by size:
395 369 353 256 208 72 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 641 674 1674
Size: 641 33 1000
Fragments arranged by size:
1000 641 33
BamHI G'GATC_C
Cuts at: 0 1123 1359 1674
Size: 1123 236 315
Fragments arranged by size:
1123 315 236
BanI G'GyrC_C
Cuts at: 0 649 1557 1674
Size: 649 908 117
Fragments arranged by size:
908 649 117
BanII G_rGCy'C
Cuts at: 0 83 1148 1426 1674
Size: 83 1065 278 248
Fragments arranged by size:
1065 278 248 83
BciVI GTATCCnnnnn_n'
Cuts at: 0 880 1674
Size: 880 794
BglI GCCn_nnn'nGGC
Cuts at: 0 1112 1421 1555 1674
Size: 1112 309 134 119
Fragments arranged by size:
1112 309 134 119
BlpI GC'TnA_GC
Cuts at: 0 284 1674
Size: 284 1390
Bme1580I G_kGCm'C
Cuts at: 0 1426 1560 1674
Size: 1426 134 114
Fragments arranged by size:
1426 134 114
BmrI ACTGGGnnnn_n'
Cuts at: 0 1458 1674
Size: 1458 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 605 1674
Size: 97 508 1069
Fragments arranged by size:
1069 508 97
Bpu10I CC'TnA_GC
Cuts at: 0 623 1674
Size: 623 1051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1674
Size: 1339 335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1674
Size: 17 30 1627
Fragments arranged by size:
1627 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 602 1674
Size: 36 566 1072
Fragments arranged by size:
1072 566 36
BseYI C'CCAG_C
Cuts at: 0 1333 1674
Size: 1333 341
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1674
Size: 351 1323
BsiHKAI G_wGCw'C
Cuts at: 0 290 1148 1674
Size: 290 858 526
Fragments arranged by size:
858 526 290
Bsp1286I G_dGCh'C
Cuts at: 0 83 290 1148 1426 1560 1674
Size: 83 207 858 278 134 114
Fragments arranged by size:
858 278 207 134 114 83
BspHI T'CATG_A
Cuts at: 0 242 795 1674
Size: 242 553 879
Fragments arranged by size:
879 553 242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 1674
Size: 94 1580
BsrDI GCAATG_nn'
Cuts at: 0 593 1289 1674
Size: 593 696 385
Fragments arranged by size:
696 593 385
BsrGI T'GTAC_A
Cuts at: 0 1033 1674
Size: 1033 641
BstYI r'GATC_y
Cuts at: 0 506 530 1123 1359 1674
Size: 506 24 593 236 315
Fragments arranged by size:
593 506 315 236 24
BtsI GCAGTG_nn'
Cuts at: 0 23 1674
Size: 23 1651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1674
Size: 72 35 1567
Fragments arranged by size:
1567 72 35
DraI TTT'AAA
Cuts at: 0 498 1260 1490 1544 1674
Size: 498 762 230 54 130
Fragments arranged by size:
762 498 230 130 54
DraIII CAC_nnn'GTG
Cuts at: 0 329 1674
Size: 329 1345
EaeI y'GGCC_r
Cuts at: 0 2 1674
Size: 2 1672
EarI CTCTTCn'nnn_
Cuts at: 0 675 1583 1674
Size: 675 908 91
Fragments arranged by size:
908 675 91
EcoICRI GAG'CTC
Cuts at: 0 1146 1674
Size: 1146 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 605 656 1674
Size: 97 508 51 1018
Fragments arranged by size:
1018 508 97 51
EcoO109I rG'GnC_Cy
Cuts at: 0 72 1423 1674
Size: 72 1351 251
Fragments arranged by size:
1351 251 72
EcoRI G'AATT_C
Cuts at: 0 485 1674
Size: 485 1189
EcoRV GAT'ATC
Cuts at: 0 1308 1674
Size: 1308 366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1036 1080 1674
Size: 1036 44 594
Fragments arranged by size:
1036 594 44
HaeII r_GCGC'y
Cuts at: 0 669 1674
Size: 669 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 777 809 1627 1659 1674
Size: 777 32 818 32 15
Fragments arranged by size:
818 777 32 32 15
KpnI G_GTAC'C
Cuts at: 0 653 1674
Size: 653 1021
MluI A'CGCG_T
Cuts at: 0 555 1674
Size: 555 1119
MscI TGG'CCA
Cuts at: 0 4 1674
Size: 4 1670
MslI CAynn'nnrTG
Cuts at: 0 598 1674
Size: 598 1076
PfoI T'CCnGG_A
Cuts at: 0 480 1674
Size: 480 1194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 638 1674
Size: 606 32 1036
Fragments arranged by size:
1036 606 32
PpuMI rG'GwC_Cy
Cuts at: 0 72 1674
Size: 72 1602
PshAI GACnn'nnGTC
Cuts at: 0 157 181 1674
Size: 157 24 1493
Fragments arranged by size:
1493 157 24
PsiI TTA'TAA
Cuts at: 0 692 1674
Size: 692 982
PspOMI G'GGCC_C
Cuts at: 0 1422 1674
Size: 1422 252
SacI G_AGCT'C
Cuts at: 0 1148 1674
Size: 1148 526
SapI GCTCTTCn'nnn_
Cuts at: 0 675 1674
Size: 675 999
ScaI AGT'ACT
Cuts at: 0 639 1674
Size: 639 1035
SfcI C'TryA_G
Cuts at: 0 717 980 1630 1674
Size: 717 263 650 44
Fragments arranged by size:
717 650 263 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1112 1674
Size: 1112 562
SmlI C'TyrA_G
Cuts at: 0 1354 1674
Size: 1354 320
TatI w'GTAC_w
Cuts at: 0 524 637 1033 1396 1674
Size: 524 113 396 363 278
Fragments arranged by size:
524 396 363 278 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 1334 1366 1674
Size: 17 32 1285 32 308
Fragments arranged by size:
1285 308 32 32 17
XmnI GAAnn'nnTTC
Cuts at: 0 476 542 1674
Size: 476 66 1132
Fragments arranged by size:
1132 476 66
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlwNI ApaI ApoI
BaeI BamHI BanI BanII BciVI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BstYI BtsI
CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I KpnI MluI MscI
MslI PfoI PpiI PpuMI PshAI PsiI PspOMI SacI
SapI ScaI SfcI SfiI SmlI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BbeI
BbsI BbvCI BcgI BclI BfrBI BglII BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
DrdI EagI EciI EcoNI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI