Restriction map for
pFN21AE6435
(Linear) (Six-base) MAPSORT of: KIEE6435.seq Check: 5067 from: 1 to: 1419
KIEE6435.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 131 1419
Size: 131 1288
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 291 506 685 1419
Size: 63 228 215 179 734
Fragments arranged by size:
734 228 215 179 63
AfeI AGC'GCT
Cuts at: 0 139 1419
Size: 139 1280
AflIII A'CryG_T
Cuts at: 0 261 1419
Size: 261 1158
AlwNI CAG_nnn'CTG
Cuts at: 0 1233 1419
Size: 1233 186
ApoI r'AATT_y
Cuts at: 0 685 695 968 1015 1185 1240 1419
Size: 685 10 273 47 170 55 179
Fragments arranged by size:
685 273 179 170 55 47 10
BamHI G'GATC_C
Cuts at: 0 174 1419
Size: 174 1245
BanII G_rGCy'C
Cuts at: 0 101 1419
Size: 101 1318
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 656 847 1419
Size: 573 83 191 572
Fragments arranged by size:
573 572 191 83
BciVI GTATCCnnnnn_n'
Cuts at: 0 66 391 1419
Size: 66 325 1028
Fragments arranged by size:
1028 325 66
BclI T'GATC_A
Cuts at: 0 224 1419
Size: 224 1195
BglII A'GATC_T
Cuts at: 0 113 1419
Size: 113 1306
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 1419
Size: 162 1257
BmtI G_CTAG'C
Cuts at: 0 1045 1419
Size: 1045 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 360 759 1419
Size: 57 303 399 660
Fragments arranged by size:
660 399 303 57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1419
Size: 55 1364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1419
Size: 608 811
BsiEI CG_ry'CG
Cuts at: 0 32 834 1419
Size: 32 802 585
Fragments arranged by size:
802 585 32
Bsp1286I G_dGCh'C
Cuts at: 0 101 1419
Size: 101 1318
BspHI T'CATG_A
Cuts at: 0 843 1419
Size: 843 576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 240 1419
Size: 131 109 1179
Fragments arranged by size:
1179 131 109
BsrDI GCAATG_nn'
Cuts at: 0 599 1419
Size: 599 820
BstEII G'GTnAC_C
Cuts at: 0 984 1419
Size: 984 435
BstYI r'GATC_y
Cuts at: 0 113 174 242 463 1419
Size: 113 61 68 221 956
Fragments arranged by size:
956 221 113 68 61
Bsu36I CC'TnA_GG
Cuts at: 0 128 1419
Size: 128 1291
DraI TTT'AAA
Cuts at: 0 732 1419
Size: 732 687
EaeI y'GGCC_r
Cuts at: 0 29 1384 1419
Size: 29 1355 35
Fragments arranged by size:
1355 35 29
EagI C'GGCC_G
Cuts at: 0 29 1419
Size: 29 1390
EarI CTCTTCn'nnn_
Cuts at: 0 73 310 1419
Size: 73 237 1109
Fragments arranged by size:
1109 237 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 1419
Size: 63 1356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 291 506 685 1419
Size: 63 228 215 179 734
Fragments arranged by size:
734 228 215 179 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 668 1419
Size: 127 541 751
Fragments arranged by size:
751 541 127
EcoRI G'AATT_C
Cuts at: 0 685 968 1419
Size: 685 283 451
Fragments arranged by size:
685 451 283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 673 871 1419
Size: 673 198 548
Fragments arranged by size:
673 548 198
HaeII r_GCGC'y
Cuts at: 0 141 1419
Size: 141 1278
HincII GTy'rAC
Cuts at: 0 398 614 1419
Size: 398 216 805
Fragments arranged by size:
805 398 216
HpaI GTT'AAC
Cuts at: 0 614 1419
Size: 614 805
MfeI C'AATT_G
Cuts at: 0 215 1419
Size: 215 1204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1123 1419
Size: 445 678 296
Fragments arranged by size:
678 445 296
MscI TGG'CCA
Cuts at: 0 1386 1419
Size: 1386 33
MslI CAynn'nnrTG
Cuts at: 0 280 1419
Size: 280 1139
MspA1I CmG'CkG
Cuts at: 0 9 1419
Size: 9 1410
NdeI CA'TA_TG
Cuts at: 0 1201 1419
Size: 1201 218
NheI G'CTAG_C
Cuts at: 0 1041 1419
Size: 1041 378
NspI r_CATG'y
Cuts at: 0 265 1419
Size: 265 1154
PciI A'CATG_T
Cuts at: 0 261 1419
Size: 261 1158
PvuI CG_AT'CG
Cuts at: 0 834 1419
Size: 834 585
PvuII CAG'CTG
Cuts at: 0 9 1419
Size: 9 1410
SexAI A'CCwGG_T
Cuts at: 0 206 1419
Size: 206 1213
SmlI C'TyrA_G
Cuts at: 0 36 375 738 1419
Size: 36 339 363 681
Fragments arranged by size:
681 363 339 36
SspI AAT'ATT
Cuts at: 0 1111 1419
Size: 1111 308
StyI C'CwwG_G
Cuts at: 0 531 1419
Size: 531 888
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApoI BamHI BanII
BbsI BciVI BclI BglII BmrI BmtI BpuEI BseRI
BsgI BsiEI Bsp1286I BspHI BspMI BsrDI BstEII BstYI
Bsu36I DraI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI HaeII HincII HpaI MfeI MmeI MscI
MslI MspA1I NdeI NheI NspI PciI PvuI PvuII
SexAI SmlI SspI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EcoICRI EcoO109I EcoRV FseI FspI FspAI
Hin4I HindIII KasI KpnI MluI NaeI NarI NcoI
NgoMIV NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI