Restriction map for
pFN21AE6434
(Linear) (Six-base) MAPSORT of: KIEE6434.seq Check: 1826 from: 1 to: 1644
KIEE6434.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 1644
Size: 15 1629
AccI GT'mk_AC
Cuts at: 0 1178 1644
Size: 1178 466
Acc65I G'GTAC_C
Cuts at: 0 752 1644
Size: 752 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 924 1343 1346 1591 1644
Size: 156 768 419 3 245 53
Fragments arranged by size:
768 419 245 156 53 3
AflIII A'CryG_T
Cuts at: 0 1121 1644
Size: 1121 523
AleI CACnn'nnGTG
Cuts at: 0 143 1644
Size: 143 1501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1276 1644
Size: 1276 368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 968 1000 1644
Size: 968 32 644
Fragments arranged by size:
968 644 32
AlwNI CAG_nnn'CTG
Cuts at: 0 236 824 1644
Size: 236 588 820
Fragments arranged by size:
820 588 236
ApaI G_GGCC'C
Cuts at: 0 44 1644
Size: 44 1600
ApoI r'AATT_y
Cuts at: 0 367 1644
Size: 367 1277
AvaI C'yCGr_G
Cuts at: 0 80 209 1644
Size: 80 129 1435
Fragments arranged by size:
1435 129 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 525 558 1644
Size: 525 33 1086
Fragments arranged by size:
1086 525 33
BanI G'GyrC_C
Cuts at: 0 157 752 831 1519 1644
Size: 157 595 79 688 125
Fragments arranged by size:
688 595 157 125 79
BanII G_rGCy'C
Cuts at: 0 44 1432 1644
Size: 44 1388 212
Fragments arranged by size:
1388 212 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 996 1597 1644
Size: 996 601 47
Fragments arranged by size:
996 601 47
BbvCI CC'TCA_GC
Cuts at: 0 817 1644
Size: 817 827
BglI GCCn_nnn'nGGC
Cuts at: 0 1444 1510 1644
Size: 1444 66 134
Fragments arranged by size:
1444 134 66
BlpI GC'TnA_GC
Cuts at: 0 229 618 1644
Size: 229 389 1026
Fragments arranged by size:
1026 389 229
Bme1580I G_kGCm'C
Cuts at: 0 44 150 162 335 807 1237 1596 1644
Size: 44 106 12 173 472 430 359 48
Fragments arranged by size:
472 430 359 173 106 48 44 12
BmgBI CAC'GTC
Cuts at: 0 1578 1644
Size: 1578 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 390 1644
Size: 390 1254
BmtI G_CTAG'C
Cuts at: 0 787 1644
Size: 787 857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 214 331 676 682 716 1308 1644
Size: 40 174 117 345 6 34 592 336
Fragments arranged by size:
592 345 336 174 117 40 34 6
Bpu10I CC'TnA_GC
Cuts at: 0 703 817 1644
Size: 703 114 827
Fragments arranged by size:
827 703 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1558 1644
Size: 1558 86
BsaAI yAC'GTr
Cuts at: 0 537 1644
Size: 537 1107
BsaHI Gr'CG_yC
Cuts at: 0 516 1203 1644
Size: 516 687 441
Fragments arranged by size:
687 516 441
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 649 679 1010 1040 1644
Size: 649 30 331 30 604
Fragments arranged by size:
649 604 331 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 363 1644
Size: 25 338 1281
Fragments arranged by size:
1281 338 25
BseYI C'CCAG_C
Cuts at: 0 347 552 1110 1230 1422 1644
Size: 347 205 558 120 192 222
Fragments arranged by size:
558 347 222 205 192 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 827 1391 1644
Size: 82 745 564 253
Fragments arranged by size:
745 564 253 82
BsiHKAI G_wGCw'C
Cuts at: 0 683 1294 1382 1644
Size: 683 611 88 262
Fragments arranged by size:
683 611 262 88
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1534 1644
Size: 1534 110
Bsp1286I G_dGCh'C
Cuts at: 0 44 150 162 335 683 807 1237 1294
Size: 44 106 12 173 348 124 430 57
Cuts at: 1294 1382 1432 1596 1644
Size: 88 50 164 48
Fragments arranged by size:
430 348 173 164 124 106 88 57
50 48 44 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 330 1623 1644
Size: 15 315 1293 21
Fragments arranged by size:
1293 315 21 15
BsrDI GCAATG_nn'
Cuts at: 0 380 1644
Size: 380 1264
BsrFI r'CCGG_y
Cuts at: 0 457 1418 1516 1644
Size: 457 961 98 128
Fragments arranged by size:
961 457 128 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 1173 1644
Size: 120 1053 471
Fragments arranged by size:
1053 471 120
BstYI r'GATC_y
Cuts at: 0 402 1644
Size: 402 1242
BtgI C'CryG_G
Cuts at: 0 300 689 1021 1644
Size: 300 389 332 623
Fragments arranged by size:
623 389 332 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 786 1644
Size: 786 858
BtsI GCAGTG_nn'
Cuts at: 0 64 94 1225 1310 1340 1474 1644
Size: 64 30 1131 85 30 134 170
Fragments arranged by size:
1131 170 134 85 64 30 30
EaeI y'GGCC_r
Cuts at: 0 919 965 1644
Size: 919 46 679
Fragments arranged by size:
919 679 46
EarI CTCTTCn'nnn_
Cuts at: 0 1610 1644
Size: 1610 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 156 214 331 676 682 716 924
Size: 40 116 58 117 345 6 34 208
Cuts at: 924 1308 1343 1346 1591 1644
Size: 384 35 3 245 53
Fragments arranged by size:
384 345 245 208 117 116 58 53
40 35 34 6 3
EcoO109I rG'GnC_Cy
Cuts at: 0 41 1644
Size: 41 1603
EcoRI G'AATT_C
Cuts at: 0 367 1644
Size: 367 1277
EcoRV GAT'ATC
Cuts at: 0 490 1644
Size: 490 1154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 1267 1299 1644
Size: 1193 32 42 32 345
Fragments arranged by size:
1193 345 42 32 32
HindIII A'AGCT_T
Cuts at: 0 1316 1483 1644
Size: 1316 167 161
Fragments arranged by size:
1316 167 161
KpnI G_GTAC'C
Cuts at: 0 756 1644
Size: 756 888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 521 1053 1644
Size: 72 449 532 591
Fragments arranged by size:
591 532 449 72
MslI CAynn'nnrTG
Cuts at: 0 143 182 643 646 1644
Size: 143 39 461 3 998
Fragments arranged by size:
998 461 143 39 3
MspA1I CmG'CkG
Cuts at: 0 1137 1557 1644
Size: 1137 420 87
Fragments arranged by size:
1137 420 87
NaeI GCC'GGC
Cuts at: 0 1420 1518 1644
Size: 1420 98 126
Fragments arranged by size:
1420 126 98
NgoMIV G'CCGG_C
Cuts at: 0 1418 1516 1644
Size: 1418 98 128
Fragments arranged by size:
1418 128 98
NheI G'CTAG_C
Cuts at: 0 783 1644
Size: 783 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 1578 1644
Size: 1578 66
PfoI T'CCnGG_A
Cuts at: 0 1285 1571 1644
Size: 1285 286 73
Fragments arranged by size:
1285 286 73
PspOMI G'GGCC_C
Cuts at: 0 40 1644
Size: 40 1604
PstI C_TGCA'G
Cuts at: 0 570 1644
Size: 570 1074
PvuII CAG'CTG
Cuts at: 0 1137 1557 1644
Size: 1137 420 87
Fragments arranged by size:
1137 420 87
SfcI C'TryA_G
Cuts at: 0 566 1644
Size: 566 1078
SgrAI Cr'CCGG_yG
Cuts at: 0 457 1644
Size: 457 1187
SmaI CCC'GGG
Cuts at: 0 211 1644
Size: 211 1433
StyI C'CwwG_G
Cuts at: 0 861 1644
Size: 861 783
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 819 1644
Size: 819 825
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 394 426 649 681 1010 1042
Size: 86 32 276 32 223 32 329 32
Cuts at: 1042 1450 1482 1644
Size: 408 32 162
Fragments arranged by size:
408 329 276 223 162 86 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1575 1644
Size: 276 1299 69
Fragments arranged by size:
1299 276 69
XmaI C'CCGG_G
Cuts at: 0 209 1644
Size: 209 1435
XmnI GAAnn'nnTTC
Cuts at: 0 993 1644
Size: 993 651
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV Hin4I HindIII KpnI MmeI MslI MspA1I NaeI
NgoMIV NheI PflMI PfoI PspOMI PstI PvuII SfcI
SgrAI SmaI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI ApaLI AscI
AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI
BfrBI BglII BplI BpuEI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI FalI FseI FspI
FspAI HaeII HincII HpaI KasI MfeI MluI MscI
NarI NcoI NdeI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI ZraI