Restriction map for
pFN21AE6431
(Linear) (Six-base) MAPSORT of: KIEE6431.seq Check: 9028 from: 1 to: 1380
KIEE6431.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AatII G_ACGT'C
Cuts at: 0 352 1380
Size: 352 1028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 547 707 718 1164 1380
Size: 203 344 160 11 446 216
Fragments arranged by size:
446 344 216 203 160 11
AleI CACnn'nnGTG
Cuts at: 0 1012 1380
Size: 1012 368
AlwNI CAG_nnn'CTG
Cuts at: 0 744 1380
Size: 744 636
ApaI G_GGCC'C
Cuts at: 0 66 1369 1380
Size: 66 1303 11
Fragments arranged by size:
1303 66 11
ApoI r'AATT_y
Cuts at: 0 711 862 883 1380
Size: 711 151 21 497
Fragments arranged by size:
711 497 151 21
AvaI C'yCGr_G
Cuts at: 0 254 1380
Size: 254 1126
BanI G'GyrC_C
Cuts at: 0 1077 1380
Size: 1077 303
BanII G_rGCy'C
Cuts at: 0 66 1094 1369 1380
Size: 66 1028 275 11
Fragments arranged by size:
1028 275 66 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 695 1380
Size: 695 685
BclI T'GATC_A
Cuts at: 0 809 1380
Size: 809 571
Bme1580I G_kGCm'C
Cuts at: 0 66 1369 1380
Size: 66 1303 11
Fragments arranged by size:
1303 66 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 149 1380
Size: 149 1231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 156 1380
Size: 156 1224
Bpu10I CC'TnA_GC
Cuts at: 0 656 1067 1380
Size: 656 411 313
Fragments arranged by size:
656 411 313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1380
Size: 1130 250
BsaHI Gr'CG_yC
Cuts at: 0 4 349 1380
Size: 4 345 1031
Fragments arranged by size:
1031 345 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1048 1246 1380
Size: 1048 198 134
Fragments arranged by size:
1048 198 134
BseYI C'CCAG_C
Cuts at: 0 291 1343 1380
Size: 291 1052 37
Fragments arranged by size:
1052 291 37
BsiEI CG_ry'CG
Cuts at: 0 112 1380
Size: 112 1268
BsiHKAI G_wGCw'C
Cuts at: 0 1094 1380
Size: 1094 286
Bsp1286I G_dGCh'C
Cuts at: 0 66 1094 1369 1380
Size: 66 1028 275 11
Fragments arranged by size:
1028 275 66 11
BsrBI CCG'CTC
Cuts at: 0 69 1380
Size: 69 1311
BssSI C'ACGA_G
Cuts at: 0 130 261 572 1380
Size: 130 131 311 808
Fragments arranged by size:
808 311 131 130
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1197 1380
Size: 1197 183
BtsI GCAGTG_nn'
Cuts at: 0 1061 1380
Size: 1061 319
DraI TTT'AAA
Cuts at: 0 1275 1380
Size: 1275 105
DraIII CAC_nnn'GTG
Cuts at: 0 135 1228 1380
Size: 135 1093 152
Fragments arranged by size:
1093 152 135
EcoICRI GAG'CTC
Cuts at: 0 1092 1380
Size: 1092 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 547 707 718 1164 1380
Size: 203 344 160 11 446 216
Fragments arranged by size:
446 344 216 203 160 11
EcoO109I rG'GnC_Cy
Cuts at: 0 63 972 1041 1380
Size: 63 909 69 339
Fragments arranged by size:
909 339 69 63
EcoRV GAT'ATC
Cuts at: 0 1053 1380
Size: 1053 327
MfeI C'AATT_G
Cuts at: 0 213 1380
Size: 213 1167
MslI CAynn'nnrTG
Cuts at: 0 1012 1380
Size: 1012 368
MspA1I CmG'CkG
Cuts at: 0 897 1380
Size: 897 483
NspI r_CATG'y
Cuts at: 0 240 332 1351 1380
Size: 240 92 1019 29
Fragments arranged by size:
1019 240 92 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 577 1350 1380
Size: 577 773 30
Fragments arranged by size:
773 577 30
PpuMI rG'GwC_Cy
Cuts at: 0 972 1041 1380
Size: 972 69 339
Fragments arranged by size:
972 339 69
PspOMI G'GGCC_C
Cuts at: 0 62 1365 1380
Size: 62 1303 15
Fragments arranged by size:
1303 62 15
PstI C_TGCA'G
Cuts at: 0 17 1380
Size: 17 1363
PvuII CAG'CTG
Cuts at: 0 897 1380
Size: 897 483
RsrII CG'GwC_CG
Cuts at: 0 21 1380
Size: 21 1359
SacI G_AGCT'C
Cuts at: 0 1094 1380
Size: 1094 286
SanDI GG'GwC_CC
Cuts at: 0 1041 1380
Size: 1041 339
SfcI C'TryA_G
Cuts at: 0 13 380 1380
Size: 13 367 1000
Fragments arranged by size:
1000 367 13
SmlI C'TyrA_G
Cuts at: 0 1145 1380
Size: 1145 235
SphI G_CATG'C
Cuts at: 0 240 1380
Size: 240 1140
SspI AAT'ATT
Cuts at: 0 448 1380
Size: 448 932
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1024 1357 1380
Size: 1024 333 23
Fragments arranged by size:
1024 333 23
XbaI T'CTAG_A
Cuts at: 0 352 1380
Size: 352 1028
ZraI GAC'GTC
Cuts at: 0 350 1380
Size: 350 1030
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BclI Bme1580I BmrI BplI Bpu10I BpuEI
BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BssSI
BstXI BtsI DraI DraIII EcoICRI Eco57MI EcoO109I EcoRV
MfeI MslI MspA1I NspI PflMI PpuMI PspOMI PstI
PvuII RsrII SacI SanDI SfcI SmlI SphI SspI
TaqII XbaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BpmI BsaI BsaAI BsaBI
BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI
EaeI EagI EarI EciI EcoNI EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
StyI SwaI TatI TstI Tth111I XcmI XhoI XmaI
XmnI