Restriction map for
pFN21AE6430
(Linear) (Six-base) MAPSORT of: KIEE6430.seq Check: 4888 from: 1 to: 1842
KIEE6430.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1842
Size: 1096 746
AflIII A'CryG_T
Cuts at: 0 1045 1842
Size: 1045 797
AleI CACnn'nnGTG
Cuts at: 0 1630 1842
Size: 1630 212
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1842
Size: 1119 723
ApoI r'AATT_y
Cuts at: 0 994 1842
Size: 994 848
AvaI C'yCGr_G
Cuts at: 0 1396 1754 1842
Size: 1396 358 88
Fragments arranged by size:
1396 358 88
BamHI G'GATC_C
Cuts at: 0 1413 1842
Size: 1413 429
BanI G'GyrC_C
Cuts at: 0 1510 1842
Size: 1510 332
BanII G_rGCy'C
Cuts at: 0 1178 1842
Size: 1178 664
BbvCI CC'TCA_GC
Cuts at: 0 311 716 1842
Size: 311 405 1126
Fragments arranged by size:
1126 405 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1289 1842
Size: 1255 34 553
Fragments arranged by size:
1255 553 34
BclI T'GATC_A
Cuts at: 0 872 1842
Size: 872 970
BlpI GC'TnA_GC
Cuts at: 0 320 1842
Size: 320 1522
BmgBI CAC'GTC
Cuts at: 0 708 1842
Size: 708 1134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 745 1141 1192 1842
Size: 205 540 396 51 650
Fragments arranged by size:
650 540 396 205 51
Bpu10I CC'TnA_GC
Cuts at: 0 311 563 716 1842
Size: 311 252 153 1126
Fragments arranged by size:
1126 311 252 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1626 1842
Size: 1626 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 1100 1842
Size: 151 949 742
Fragments arranged by size:
949 742 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 811 841 1842
Size: 184 30 597 30 1001
Fragments arranged by size:
1001 597 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 1842
Size: 202 1640
BseYI C'CCAG_C
Cuts at: 0 505 788 1842
Size: 505 283 1054
Fragments arranged by size:
1054 505 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1842
Size: 957 885
BsiHKAI G_wGCw'C
Cuts at: 0 1178 1397 1842
Size: 1178 219 445
Fragments arranged by size:
1178 445 219
BsmI GAATG_Cn'
Cuts at: 0 396 1206 1219 1553 1842
Size: 396 810 13 334 289
Fragments arranged by size:
810 396 334 289 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 467 909 980 1451 1842
Size: 467 442 71 471 391
Fragments arranged by size:
471 467 442 391 71
Bsp1286I G_dGCh'C
Cuts at: 0 1178 1397 1842
Size: 1178 219 445
Fragments arranged by size:
1178 445 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1481 1842
Size: 1481 361
BsrGI T'GTAC_A
Cuts at: 0 1048 1842
Size: 1048 794
BstYI r'GATC_y
Cuts at: 0 756 1413 1572 1842
Size: 756 657 159 270
Fragments arranged by size:
756 657 270 159
BtgI C'CryG_G
Cuts at: 0 1682 1842
Size: 1682 160
BtsI GCAGTG_nn'
Cuts at: 0 1485 1842
Size: 1485 357
DraIII CAC_nnn'GTG
Cuts at: 0 72 961 1842
Size: 72 889 881
Fragments arranged by size:
889 881 72
EaeI y'GGCC_r
Cuts at: 0 1248 1842
Size: 1248 594
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 289 1386 1842
Size: 289 1097 456
Fragments arranged by size:
1097 456 289
EcoICRI GAG'CTC
Cuts at: 0 1176 1842
Size: 1176 666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 745 1096 1141 1192 1842
Size: 205 540 351 45 51 650
Fragments arranged by size:
650 540 351 205 51 45
EcoO109I rG'GnC_Cy
Cuts at: 0 1407 1493 1536 1806 1826 1842
Size: 1407 86 43 270 20 16
Fragments arranged by size:
1407 270 86 43 20 16
EcoRI G'AATT_C
Cuts at: 0 994 1842
Size: 994 848
EcoRV GAT'ATC
Cuts at: 0 669 1842
Size: 669 1173
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 1842
Size: 846 996
FspI TGC'GCA
Cuts at: 0 1231 1842
Size: 1231 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 1711 1743 1842
Size: 1405 32 274 32 99
Fragments arranged by size:
1405 274 99 32 32
HincII GTy'rAC
Cuts at: 0 1424 1842
Size: 1424 418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1842
Size: 81 1761
MscI TGG'CCA
Cuts at: 0 1250 1842
Size: 1250 592
MslI CAynn'nnrTG
Cuts at: 0 1630 1842
Size: 1630 212
NcoI C'CATG_G
Cuts at: 0 1682 1842
Size: 1682 160
NspI r_CATG'y
Cuts at: 0 1049 1842
Size: 1049 793
PciI A'CATG_T
Cuts at: 0 1045 1842
Size: 1045 797
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 1799 1842
Size: 618 1181 43
Fragments arranged by size:
1181 618 43
PfoI T'CCnGG_A
Cuts at: 0 1409 1495 1842
Size: 1409 86 347
Fragments arranged by size:
1409 347 86
PpuMI rG'GwC_Cy
Cuts at: 0 1407 1493 1536 1806 1826 1842
Size: 1407 86 43 270 20 16
Fragments arranged by size:
1407 270 86 43 20 16
PspXI vC'TCGA_Gb
Cuts at: 0 1396 1842
Size: 1396 446
PstI C_TGCA'G
Cuts at: 0 912 1492 1545 1842
Size: 912 580 53 297
Fragments arranged by size:
912 580 297 53
SacI G_AGCT'C
Cuts at: 0 1178 1842
Size: 1178 664
SanDI GG'GwC_CC
Cuts at: 0 1536 1842
Size: 1536 306
SbfI CC_TGCA'GG
Cuts at: 0 1545 1842
Size: 1545 297
ScaI AGT'ACT
Cuts at: 0 829 1842
Size: 829 1013
SexAI A'CCwGG_T
Cuts at: 0 611 1842
Size: 611 1231
SfcI C'TryA_G
Cuts at: 0 908 1488 1541 1842
Size: 908 580 53 301
Fragments arranged by size:
908 580 301 53
SmaI CCC'GGG
Cuts at: 0 1756 1842
Size: 1756 86
SmlI C'TyrA_G
Cuts at: 0 1396 1605 1842
Size: 1396 209 237
Fragments arranged by size:
1396 237 209
SspI AAT'ATT
Cuts at: 0 437 459 1842
Size: 437 22 1383
Fragments arranged by size:
1383 437 22
StuI AGG'CCT
Cuts at: 0 485 1842
Size: 485 1357
StyI C'CwwG_G
Cuts at: 0 1343 1682 1842
Size: 1343 339 160
Fragments arranged by size:
1343 339 160
TatI w'GTAC_w
Cuts at: 0 827 894 1048 1842
Size: 827 67 154 794
Fragments arranged by size:
827 794 154 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 748 780 1477 1509 1842
Size: 116 32 600 32 697 32 333
Fragments arranged by size:
697 600 333 116 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 238 604 706 1842
Size: 238 366 102 1136
Fragments arranged by size:
1136 366 238 102
XbaI T'CTAG_A
Cuts at: 0 1362 1842
Size: 1362 480
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 1842
Size: 1680 162
XhoI C'TCGA_G
Cuts at: 0 1396 1842
Size: 1396 446
XmaI C'CCGG_G
Cuts at: 0 1754 1842
Size: 1754 88
XmnI GAAnn'nnTTC
Cuts at: 0 1640 1842
Size: 1640 202
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanI
BanII BbvCI BcgI BclI BlpI BmgBI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrGI BstYI BtgI BtsI DraIII
EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI Hin4I HincII MmeI MscI MslI NcoI NspI
PciI PflMI PfoI PpuMI PspXI PstI SacI SanDI
SbfI ScaI SexAI SfcI SmaI SmlI SspI StuI
StyI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BciVI BfrBI BglI BglII
Bme1580I BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EarI EcoNI FseI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI PvuII RsrII SacII SalI SapI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI TaqII ZraI