Restriction map for
pFN21AE6427
(Linear) (Six-base) MAPSORT of: KIEE6427.seq Check: 6890 from: 1 to: 1863
KIEE6427.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1609 1725 1863
Size: 257 1352 116 138
Fragments arranged by size:
1352 257 138 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 549 1863
Size: 549 1314
ApaLI G'TGCA_C
Cuts at: 0 1468 1863
Size: 1468 395
ApoI r'AATT_y
Cuts at: 0 226 1012 1033 1863
Size: 226 786 21 830
Fragments arranged by size:
830 786 226 21
AvaI C'yCGr_G
Cuts at: 0 1201 1863
Size: 1201 662
AvrII C'CTAG_G
Cuts at: 0 132 309 1863
Size: 132 177 1554
Fragments arranged by size:
1554 177 132
BamHI G'GATC_C
Cuts at: 0 1352 1863
Size: 1352 511
BanII G_rGCy'C
Cuts at: 0 17 802 1673 1863
Size: 17 785 871 190
Fragments arranged by size:
871 785 190 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1863
Size: 605 1258
BciVI GTATCCnnnnn_n'
Cuts at: 0 1386 1863
Size: 1386 477
Bme1580I G_kGCm'C
Cuts at: 0 1472 1842 1863
Size: 1472 370 21
Fragments arranged by size:
1472 370 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 1863
Size: 264 1599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 1863
Size: 1754 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 928 1837 1863
Size: 433 495 909 26
Fragments arranged by size:
909 495 433 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 1863
Size: 69 1794
BsaBI GATnn'nnATC
Cuts at: 0 1303 1863
Size: 1303 560
BsaHI Gr'CG_yC
Cuts at: 0 508 1863
Size: 508 1355
BsaWI w'CCGG_w
Cuts at: 0 575 1295 1863
Size: 575 720 568
Fragments arranged by size:
720 575 568
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1532 1562 1863
Size: 1532 30 301
Fragments arranged by size:
1532 301 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 1255 1519 1588 1863
Size: 490 765 264 69 275
Fragments arranged by size:
765 490 275 264 69
BseYI C'CCAG_C
Cuts at: 0 934 1805 1863
Size: 934 871 58
Fragments arranged by size:
934 871 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1863
Size: 963 900
BsiEI CG_ry'CG
Cuts at: 0 909 1335 1527 1634 1863
Size: 909 426 192 107 229
Fragments arranged by size:
909 426 229 192 107
BsiHKAI G_wGCw'C
Cuts at: 0 521 1384 1472 1863
Size: 521 863 88 391
Fragments arranged by size:
863 521 391 88
BsiWI C'GTAC_G
Cuts at: 0 1232 1863
Size: 1232 631
BsmI GAATG_Cn'
Cuts at: 0 730 791 1863
Size: 730 61 1072
Fragments arranged by size:
1072 730 61
Bsp1286I G_dGCh'C
Cuts at: 0 17 521 802 1384 1472 1673 1842 1863
Size: 17 504 281 582 88 201 169 21
Fragments arranged by size:
582 504 281 201 169 88 21 17
BspEI T'CCGG_A
Cuts at: 0 575 1295 1863
Size: 575 720 568
Fragments arranged by size:
720 575 568
BsrBI CCG'CTC
Cuts at: 0 5 1655 1863
Size: 5 1650 208
Fragments arranged by size:
1650 208 5
BsrDI GCAATG_nn'
Cuts at: 0 521 1863
Size: 521 1342
BsrFI r'CCGG_y
Cuts at: 0 77 908 1330 1863
Size: 77 831 422 533
Fragments arranged by size:
831 533 422 77
BsrGI T'GTAC_A
Cuts at: 0 1301 1863
Size: 1301 562
BssSI C'ACGA_G
Cuts at: 0 41 1687 1863
Size: 41 1646 176
Fragments arranged by size:
1646 176 41
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 1863
Size: 442 1421
BstYI r'GATC_y
Cuts at: 0 1196 1352 1811 1863
Size: 1196 156 459 52
Fragments arranged by size:
1196 459 156 52
BtgI C'CryG_G
Cuts at: 0 29 218 1139 1458 1532 1863
Size: 29 189 921 319 74 331
Fragments arranged by size:
921 331 319 189 74 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 575 1290 1863
Size: 575 715 573
Fragments arranged by size:
715 575 573
BtsI GCAGTG_nn'
Cuts at: 0 1180 1540 1863
Size: 1180 360 323
Fragments arranged by size:
1180 360 323
DraIII CAC_nnn'GTG
Cuts at: 0 595 1863
Size: 595 1268
EaeI y'GGCC_r
Cuts at: 0 656 884 906 1332 1401 1524 1598 1631
Size: 656 228 22 426 69 123 74 33
Cuts at: 1631 1700 1863
Size: 69 163
Fragments arranged by size:
656 426 228 163 123 74 69 69
33 22
EagI C'GGCC_G
Cuts at: 0 906 1332 1524 1631 1863
Size: 906 426 192 107 232
Fragments arranged by size:
906 426 232 192 107
EarI CTCTTCn'nnn_
Cuts at: 0 22 233 298 1744 1863
Size: 22 211 65 1446 119
Fragments arranged by size:
1446 211 119 65 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1609 1725 1754 1863
Size: 257 1352 116 29 109
Fragments arranged by size:
1352 257 116 109 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 859 981 1863
Size: 859 122 882
Fragments arranged by size:
882 859 122
EcoO109I rG'GnC_Cy
Cuts at: 0 214 1863
Size: 214 1649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 1863
Size: 205 1658
FspI TGC'GCA
Cuts at: 0 25 91 1863
Size: 25 66 1772
Fragments arranged by size:
1772 66 25
HaeII r_GCGC'y
Cuts at: 0 1192 1863
Size: 1192 671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 1817 1849 1863
Size: 553 32 1232 32 14
Fragments arranged by size:
1232 553 32 32 14
HindIII A'AGCT_T
Cuts at: 0 1721 1863
Size: 1721 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 827 846 1863
Size: 386 441 19 1017
Fragments arranged by size:
1017 441 386 19
MscI TGG'CCA
Cuts at: 0 658 886 1600 1702 1863
Size: 658 228 714 102 161
Fragments arranged by size:
714 658 228 161 102
MslI CAynn'nnrTG
Cuts at: 0 397 415 1387 1863
Size: 397 18 972 476
Fragments arranged by size:
972 476 397 18
MspA1I CmG'CkG
Cuts at: 0 905 1581 1755 1863
Size: 905 676 174 108
Fragments arranged by size:
905 676 174 108
NaeI GCC'GGC
Cuts at: 0 1332 1863
Size: 1332 531
NcoI C'CATG_G
Cuts at: 0 1139 1458 1863
Size: 1139 319 405
Fragments arranged by size:
1139 405 319
NgoMIV G'CCGG_C
Cuts at: 0 1330 1863
Size: 1330 533
NspI r_CATG'y
Cuts at: 0 1330 1862 1863
Size: 1330 532 1
Fragments arranged by size:
1330 532 1
PasI CC'CwG_GG
Cuts at: 0 1422 1863
Size: 1422 441
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 635 1848 1863
Size: 553 82 1213 15
Fragments arranged by size:
1213 553 82 15
PpuMI rG'GwC_Cy
Cuts at: 0 214 1863
Size: 214 1649
PshAI GACnn'nnGTC
Cuts at: 0 688 1863
Size: 688 1175
PstI C_TGCA'G
Cuts at: 0 1196 1325 1863
Size: 1196 129 538
Fragments arranged by size:
1196 538 129
PvuII CAG'CTG
Cuts at: 0 1581 1755 1863
Size: 1581 174 108
Fragments arranged by size:
1581 174 108
RsrII CG'GwC_CG
Cuts at: 0 1529 1863
Size: 1529 334
SanDI GG'GwC_CC
Cuts at: 0 214 1863
Size: 214 1649
SapI GCTCTTCn'nnn_
Cuts at: 0 22 298 1863
Size: 22 276 1565
Fragments arranged by size:
1565 276 22
SfcI C'TryA_G
Cuts at: 0 1192 1321 1863
Size: 1192 129 542
Fragments arranged by size:
1192 542 129
SmlI C'TyrA_G
Cuts at: 0 448 943 1816 1863
Size: 448 495 873 47
Fragments arranged by size:
873 495 448 47
SphI G_CATG'C
Cuts at: 0 1330 1863
Size: 1330 533
SspI AAT'ATT
Cuts at: 0 663 1863
Size: 663 1200
StyI C'CwwG_G
Cuts at: 0 132 309 629 855 1139 1458 1863
Size: 132 177 320 226 284 319 405
Fragments arranged by size:
405 320 319 284 226 177 132
TatI w'GTAC_w
Cuts at: 0 371 1301 1863
Size: 371 930 562
Fragments arranged by size:
930 562 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1532 1564 1863
Size: 1532 32 299
Fragments arranged by size:
1532 299 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1863
Size: 840 1023
XmnI GAAnn'nnTTC
Cuts at: 0 1150 1738 1863
Size: 1150 588 125
Fragments arranged by size:
1150 588 125
Enzymes that do cut:
AcuI AlfI ApaLI ApoI AvaI AvrII BamHI BanII
BbsI BciVI Bme1580I BplI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI
BssSI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PpuMI PshAI PstI PvuII
RsrII SanDI SapI SfcI SmlI SphI SspI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI AscI AseI
AsiSI BaeI BanI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaAI
BsmBI BspHI BspMI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI EcoRI
EcoRV FseI FspAI HincII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI