Restriction map for
pFN21AE6426
(Linear) (Six-base) MAPSORT of: KIEE6426.seq Check: 8859 from: 1 to: 1572
KIEE6426.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 606 1316 1572
Size: 606 710 256
Fragments arranged by size:
710 606 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 499 606 1105 1572
Size: 403 96 107 499 467
Fragments arranged by size:
499 467 403 107 96
AfeI AGC'GCT
Cuts at: 0 1057 1572
Size: 1057 515
AflIII A'CryG_T
Cuts at: 0 569 1572
Size: 569 1003
AgeI A'CCGG_T
Cuts at: 0 36 1572
Size: 36 1536
AlwNI CAG_nnn'CTG
Cuts at: 0 540 776 805 964 1400 1572
Size: 540 236 29 159 436 172
Fragments arranged by size:
540 436 236 172 159 29
ApaI G_GGCC'C
Cuts at: 0 1394 1572
Size: 1394 178
BamHI G'GATC_C
Cuts at: 0 1305 1572
Size: 1305 267
BanI G'GyrC_C
Cuts at: 0 1037 1572
Size: 1037 535
BanII G_rGCy'C
Cuts at: 0 82 683 1394 1493 1553 1572
Size: 82 601 711 99 60 19
Fragments arranged by size:
711 601 99 82 60 19
BbvCI CC'TCA_GC
Cuts at: 0 339 1572
Size: 339 1233
BciVI GTATCCnnnnn_n'
Cuts at: 0 398 1572
Size: 398 1174
BclI T'GATC_A
Cuts at: 0 197 1511 1572
Size: 197 1314 61
Fragments arranged by size:
1314 197 61
BfrBI ATG'CAT
Cuts at: 0 617 1572
Size: 617 955
BlpI GC'TnA_GC
Cuts at: 0 10 910 1572
Size: 10 900 662
Fragments arranged by size:
900 662 10
Bme1580I G_kGCm'C
Cuts at: 0 690 1394 1572
Size: 690 704 178
Fragments arranged by size:
704 690 178
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 1572
Size: 647 925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1379 1572
Size: 1379 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 703 1572
Size: 127 576 869
Fragments arranged by size:
869 576 127
Bpu10I CC'TnA_GC
Cuts at: 0 339 1572
Size: 339 1233
BsaHI Gr'CG_yC
Cuts at: 0 454 822 1572
Size: 454 368 750
Fragments arranged by size:
750 454 368
BsaWI w'CCGG_w
Cuts at: 0 36 320 1466 1572
Size: 36 284 1146 106
Fragments arranged by size:
1146 284 106 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 1572
Size: 226 1346
BseYI C'CCAG_C
Cuts at: 0 24 273 393 1127 1179 1572
Size: 24 249 120 734 52 393
Fragments arranged by size:
734 393 249 120 52 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1188 1444 1572
Size: 1188 256 128
Fragments arranged by size:
1188 256 128
BsiHKAI G_wGCw'C
Cuts at: 0 581 683 1572
Size: 581 102 889
Fragments arranged by size:
889 581 102
Bsp1286I G_dGCh'C
Cuts at: 0 82 581 683 690 1394 1493 1553 1572
Size: 82 499 102 7 704 99 60 19
Fragments arranged by size:
704 499 102 99 82 60 19 7
BspHI T'CATG_A
Cuts at: 0 590 1572
Size: 590 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1264 1572
Size: 1264 308
BsrBI CCG'CTC
Cuts at: 0 819 1572
Size: 819 753
BsrDI GCAATG_nn'
Cuts at: 0 50 347 1572
Size: 50 297 1225
Fragments arranged by size:
1225 297 50
BsrFI r'CCGG_y
Cuts at: 0 36 137 1572
Size: 36 101 1435
Fragments arranged by size:
1435 101 36
BsrGI T'GTAC_A
Cuts at: 0 1022 1572
Size: 1022 550
BssHII G'CGCG_C
Cuts at: 0 1518 1572
Size: 1518 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1256 1572
Size: 1256 316
BstEII G'GTnAC_C
Cuts at: 0 184 1358 1572
Size: 184 1174 214
Fragments arranged by size:
1174 214 184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 885 1298 1572
Size: 675 210 413 274
Fragments arranged by size:
675 413 274 210
BstYI r'GATC_y
Cuts at: 0 1305 1572
Size: 1305 267
Bsu36I CC'TnA_GG
Cuts at: 0 1165 1310 1572
Size: 1165 145 262
Fragments arranged by size:
1165 262 145
BtgI C'CryG_G
Cuts at: 0 52 995 1421 1572
Size: 52 943 426 151
Fragments arranged by size:
943 426 151 52
ClaI AT'CG_AT
Cuts at: 0 1265 1572
Size: 1265 307
DrdI GACnn_nn'nnGTC
Cuts at: 0 237 1572
Size: 237 1335
EaeI y'GGCC_r
Cuts at: 0 242 998 1230 1300 1572
Size: 242 756 232 70 272
Fragments arranged by size:
756 272 242 232 70
EarI CTCTTCn'nnn_
Cuts at: 0 373 1572
Size: 373 1199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1441 1572
Size: 1441 131
EcoICRI GAG'CTC
Cuts at: 0 681 1572
Size: 681 891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 403 499 606 703 1105 1572
Size: 127 276 96 107 97 402 467
Fragments arranged by size:
467 402 276 127 107 97 96
EcoO109I rG'GnC_Cy
Cuts at: 0 277 773 1017 1390 1572
Size: 277 496 244 373 182
Fragments arranged by size:
496 373 277 244 182
HaeII r_GCGC'y
Cuts at: 0 765 1059 1572
Size: 765 294 513
Fragments arranged by size:
765 513 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1158 1190 1572
Size: 1158 32 382
Fragments arranged by size:
1158 382 32
HindIII A'AGCT_T
Cuts at: 0 1563 1572
Size: 1563 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1572
Size: 424 1148
MslI CAynn'nnrTG
Cuts at: 0 898 973 1150 1572
Size: 898 75 177 422
Fragments arranged by size:
898 422 177 75
MspA1I CmG'CkG
Cuts at: 0 343 1131 1572
Size: 343 788 441
Fragments arranged by size:
788 441 343
NaeI GCC'GGC
Cuts at: 0 139 1572
Size: 139 1433
NcoI C'CATG_G
Cuts at: 0 52 1421 1572
Size: 52 1369 151
Fragments arranged by size:
1369 151 52
NgoMIV G'CCGG_C
Cuts at: 0 137 1572
Size: 137 1435
NsiI A_TGCA'T
Cuts at: 0 619 1572
Size: 619 953
NspI r_CATG'y
Cuts at: 0 573 1572
Size: 573 999
PasI CC'CwG_GG
Cuts at: 0 255 1572
Size: 255 1317
PciI A'CATG_T
Cuts at: 0 569 1572
Size: 569 1003
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 1572
Size: 726 846
PfoI T'CCnGG_A
Cuts at: 0 812 1572
Size: 812 760
PpuMI rG'GwC_Cy
Cuts at: 0 277 1017 1572
Size: 277 740 555
Fragments arranged by size:
740 555 277
PspOMI G'GGCC_C
Cuts at: 0 1390 1572
Size: 1390 182
PvuII CAG'CTG
Cuts at: 0 343 1131 1572
Size: 343 788 441
Fragments arranged by size:
788 441 343
SacI G_AGCT'C
Cuts at: 0 683 1572
Size: 683 889
SanDI GG'GwC_CC
Cuts at: 0 277 1572
Size: 277 1295
SfcI C'TryA_G
Cuts at: 0 206 607 1572
Size: 206 401 965
Fragments arranged by size:
965 401 206
StyI C'CwwG_G
Cuts at: 0 52 356 596 1421 1572
Size: 52 304 240 825 151
Fragments arranged by size:
825 304 240 151 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 1055 1222 1572
Size: 369 686 167 350
Fragments arranged by size:
686 369 350 167
TatI w'GTAC_w
Cuts at: 0 1022 1572
Size: 1022 550
Tth111I GACn'n_nGTC
Cuts at: 0 805 1572
Size: 805 767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 170 1572
Size: 170 1402
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AlwNI ApaI BamHI
BanI BanII BbvCI BciVI BclI BfrBI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HindIII MmeI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PvuII SacI SanDI SfcI StyI TaqII TatI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbsI BcgI BglI BglII BmgBI BmtI
BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BssSI BstBI BstZ17I BtgZI BtsI CspCI
DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XhoI XmaI
XmnI ZraI