(Linear) (Six-base) MAPSORT of: KIEE6425.seq  Check: 7409  from: 1  to: 1329



KIEE6425.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     921    1329 
   Size:        921     408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638    1329 
   Size:        638     691
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     746    1329 
   Size:        746     583
 
AlwNI CAG_nnn'CTG

Cuts at:      0     686    1329 
   Size:        686     643
 
ApaLI G'TGCA_C

Cuts at:      0    1150    1329 
   Size:       1150     179
 
ApoI r'AATT_y

Cuts at:      0     929    1005    1329 
   Size:        929      76     324

  Fragments arranged by size: 

                 929     324      76
 
AvaI C'yCGr_G

Cuts at:      0    1067    1329 
   Size:       1067     262
 
BanII G_rGCy'C

Cuts at:      0     716    1329 
   Size:        716     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     364    1287    1329 
   Size:        364     923      42

  Fragments arranged by size: 

                 923     364      42
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1244    1278    1329 
   Size:       1244      34      51

  Fragments arranged by size: 

                1244      51      34
 
Bme1580I G_kGCm'C

Cuts at:      0     760    1154    1329 
   Size:        760     394     175

  Fragments arranged by size: 

                 760     394     175
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1267    1329 
   Size:       1267      62
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1063    1329 
   Size:       1063     266
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     811     944    1329 
   Size:        730      81     133     385

  Fragments arranged by size: 

                 730     385     133      81
 
BsaWI w'CCGG_w

Cuts at:      0     905    1329 
   Size:        905     424
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142    1329 
   Size:        112      30    1187

  Fragments arranged by size: 

                1187     112      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     797    1329 
   Size:        797     532
 
BseYI C'CCAG_C

Cuts at:      0     387    1329 
   Size:        387     942
 
BsiHKAI G_wGCw'C

Cuts at:      0    1154    1329 
   Size:       1154     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     716     760    1154    1329 
   Size:        716      44     394     175

  Fragments arranged by size: 

                 716     394     175      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     921    1329 
   Size:        921     408
 
BsrDI GCAATG_nn'

Cuts at:      0     728     923    1329 
   Size:        728     195     406

  Fragments arranged by size: 

                 728     406     195
 
BstBI TT'CG_AA

Cuts at:      0    1191    1329 
   Size:       1191     138
 
BstEII G'GTnAC_C

Cuts at:      0     908    1329 
   Size:        908     421
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     750    1329 
   Size:        750     579
 
BstYI r'GATC_y

Cuts at:      0      50     889    1025    1329 
   Size:         50     839     136     304

  Fragments arranged by size: 

                 839     304     136      50
 
DraI TTT'AAA

Cuts at:      0     293     846    1329 
   Size:        293     553     483

  Fragments arranged by size: 

                 553     483     293
 
EarI CTCTTCn'nnn_

Cuts at:      0     993    1329 
   Size:        993     336
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638     730     811     944    1329 
   Size:        638      92      81     133     385

  Fragments arranged by size: 

                 638     385     133      92      81
 
EcoO109I rG'GnC_Cy

Cuts at:      0      35     738    1329 
   Size:         35     703     591

  Fragments arranged by size: 

                 703     591      35
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     839    1329 
   Size:        839     490
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144    1329 
   Size:        112      32    1185

  Fragments arranged by size: 

                1185     112      32
 
HindIII A'AGCT_T

Cuts at:      0     324    1329 
   Size:        324    1005
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1286    1329 
   Size:       1286      43
 
MslI CAynn'nnrTG

Cuts at:      0      89    1275    1329 
   Size:         89    1186      54

  Fragments arranged by size: 

                1186      89      54
 
MspA1I CmG'CkG

Cuts at:      0     101    1329 
   Size:        101    1228
 
NruI TCG'CGA

Cuts at:      0    1232    1329 
   Size:       1232      97
 
NspI r_CATG'y

Cuts at:      0     727    1329 
   Size:        727     602
 
PfoI T'CCnGG_A

Cuts at:      0     674    1329 
   Size:        674     655
 
PpuMI rG'GwC_Cy

Cuts at:      0      35     738    1329 
   Size:         35     703     591

  Fragments arranged by size: 

                 703     591      35
 
PsiI TTA'TAA

Cuts at:      0    1054    1329 
   Size:       1054     275
 
PvuII CAG'CTG

Cuts at:      0     101    1329 
   Size:        101    1228
 
SmlI C'TyrA_G

Cuts at:      0    1067    1329 
   Size:       1067     262
 
SphI G_CATG'C

Cuts at:      0     727    1329 
   Size:        727     602
 
SspI AAT'ATT

Cuts at:      0    1227    1329 
   Size:       1227     102
 
StyI C'CwwG_G

Cuts at:      0     734    1329 
   Size:        734     595
 
SwaI ATTT'AAAT

Cuts at:      0     846    1329 
   Size:        846     483
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     137     169     293     325    1329 
   Size:        137      32     124      32    1004

  Fragments arranged by size: 

                1004     137     124      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     459    1329 
   Size:        459     870
 
XhoI C'TCGA_G

Cuts at:      0    1067    1329 
   Size:       1067     262
 
XmnI GAAnn'nnTTC

Cuts at:      0     572    1104    1329 
   Size:        572     532     225

  Fragments arranged by size: 

                 572     532     225
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI    BanII
     BbsI     BcgI Bme1580I     BmrI     BplI     BpmI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstBI   BstEII
    BstXI    BstYI     DraI     EarI  Eco57MI EcoO109I     FalI    Hin4I
  HindIII     MmeI     MslI   MspA1I     NruI     NspI     PfoI    PpuMI
     PsiI    PvuII     SmlI     SphI     SspI     StyI     SwaI     TstI
  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI    TaqII     TatI     XbaI     XcmI     XmaI
     ZraI