Restriction map for
pFN21AE6422
(Linear) (Six-base) MAPSORT of: KIEE6422.seq Check: 4369 from: 1 to: 1503
KIEE6422.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1503
Size: 310 1193
AleI CACnn'nnGTG
Cuts at: 0 1190 1503
Size: 1190 313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 1503
Size: 319 32 1152
Fragments arranged by size:
1152 319 32
AlwNI CAG_nnn'CTG
Cuts at: 0 136 520 1059 1314 1503
Size: 136 384 539 255 189
Fragments arranged by size:
539 384 255 189 136
ApaI G_GGCC'C
Cuts at: 0 1443 1503
Size: 1443 60
ApaLI G'TGCA_C
Cuts at: 0 968 1040 1503
Size: 968 72 463
Fragments arranged by size:
968 463 72
ApoI r'AATT_y
Cuts at: 0 762 1348 1503
Size: 762 586 155
Fragments arranged by size:
762 586 155
AvaI C'yCGr_G
Cuts at: 0 925 1503
Size: 925 578
BamHI G'GATC_C
Cuts at: 0 201 1503
Size: 201 1302
BanI G'GyrC_C
Cuts at: 0 473 508 1048 1503
Size: 473 35 540 455
Fragments arranged by size:
540 473 455 35
BanII G_rGCy'C
Cuts at: 0 245 668 1214 1443 1503
Size: 245 423 546 229 60
Fragments arranged by size:
546 423 245 229 60
BbvCI CC'TCA_GC
Cuts at: 0 197 1503
Size: 197 1306
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 481 747 781 1269 1303 1503
Size: 447 34 266 34 488 34 200
Fragments arranged by size:
488 447 266 200 34 34 34
BfrBI ATG'CAT
Cuts at: 0 408 1503
Size: 408 1095
BglI GCCn_nnn'nGGC
Cuts at: 0 826 1503
Size: 826 677
BglII A'GATC_T
Cuts at: 0 845 1503
Size: 845 658
BlpI GC'TnA_GC
Cuts at: 0 991 1503
Size: 991 512
Bme1580I G_kGCm'C
Cuts at: 0 230 972 1044 1053 1443 1503
Size: 230 742 72 9 390 60
Fragments arranged by size:
742 390 230 72 60 9
BmgBI CAC'GTC
Cuts at: 0 157 576 1503
Size: 157 419 927
Fragments arranged by size:
927 419 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 913 1047 1503
Size: 697 216 134 456
Fragments arranged by size:
697 456 216 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 499 739 1503
Size: 98 401 240 764
Fragments arranged by size:
764 401 240 98
Bpu10I CC'TnA_GC
Cuts at: 0 197 709 1503
Size: 197 512 794
Fragments arranged by size:
794 512 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1503
Size: 826 677
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 1503
Size: 953 550
BsaAI yAC'GTr
Cuts at: 0 429 561 1503
Size: 429 132 942
Fragments arranged by size:
942 429 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 790 820 1503
Size: 55 30 705 30 683
Fragments arranged by size:
705 683 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 223 676 736 943 1503
Size: 36 187 453 60 207 560
Fragments arranged by size:
560 453 207 187 60 36
BseYI C'CCAG_C
Cuts at: 0 37 611 1450 1503
Size: 37 574 839 53
Fragments arranged by size:
839 574 53 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1168 1503
Size: 619 549 335
Fragments arranged by size:
619 549 335
BsiEI CG_ry'CG
Cuts at: 0 622 1503
Size: 622 881
BsiHKAI G_wGCw'C
Cuts at: 0 245 972 1016 1044 1241 1503
Size: 245 727 44 28 197 262
Fragments arranged by size:
727 262 245 197 44 28
BsmI GAATG_Cn'
Cuts at: 0 248 1503
Size: 248 1255
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1114 1503
Size: 1114 389
Bsp1286I G_dGCh'C
Cuts at: 0 230 245 668 972 1016 1044 1053 1214
Size: 230 15 423 304 44 28 9 161
Cuts at: 1214 1241 1443 1503
Size: 27 202 60
Fragments arranged by size:
423 304 230 202 161 60 44 28
27 15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 545 1503
Size: 494 51 958
Fragments arranged by size:
958 494 51
BsrBI CCG'CTC
Cuts at: 0 471 546 1503
Size: 471 75 957
Fragments arranged by size:
957 471 75
BsrDI GCAATG_nn'
Cuts at: 0 191 1203 1503
Size: 191 1012 300
Fragments arranged by size:
1012 300 191
BsrFI r'CCGG_y
Cuts at: 0 594 1503
Size: 594 909
BssSI C'ACGA_G
Cuts at: 0 971 1503
Size: 971 532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1048 1503
Size: 1048 455
BstBI TT'CG_AA
Cuts at: 0 900 1503
Size: 900 603
BstYI r'GATC_y
Cuts at: 0 201 845 1503
Size: 201 644 658
Fragments arranged by size:
658 644 201
BtgI C'CryG_G
Cuts at: 0 438 963 1503
Size: 438 525 540
Fragments arranged by size:
540 525 438
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 394 1503
Size: 394 1109
BtsI GCAGTG_nn'
Cuts at: 0 1472 1503
Size: 1472 31
DraIII CAC_nnn'GTG
Cuts at: 0 1048 1503
Size: 1048 455
DrdI GACnn_nn'nnGTC
Cuts at: 0 919 1503
Size: 919 584
EaeI y'GGCC_r
Cuts at: 0 218 619 1134 1398 1503
Size: 218 401 515 264 105
Fragments arranged by size:
515 401 264 218 105
EagI C'GGCC_G
Cuts at: 0 619 1503
Size: 619 884
EarI CTCTTCn'nnn_
Cuts at: 0 784 790 1503
Size: 784 6 713
Fragments arranged by size:
784 713 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 581 1503
Size: 581 922
EcoICRI GAG'CTC
Cuts at: 0 243 1503
Size: 243 1260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 310 499 739 1503
Size: 98 212 189 240 764
Fragments arranged by size:
764 240 212 189 98
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 1503
Size: 1231 272
EcoO109I rG'GnC_Cy
Cuts at: 0 321 1404 1440 1503
Size: 321 1083 36 63
Fragments arranged by size:
1083 321 63 36
FspI TGC'GCA
Cuts at: 0 565 1503
Size: 565 938
HaeII r_GCGC'y
Cuts at: 0 980 1503
Size: 980 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 790 822 1503
Size: 55 32 703 32 681
Fragments arranged by size:
703 681 55 32 32
HincII GTy'rAC
Cuts at: 0 675 1466 1503
Size: 675 791 37
Fragments arranged by size:
791 675 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 157 659 1503
Size: 11 146 502 844
Fragments arranged by size:
844 502 146 11
MscI TGG'CCA
Cuts at: 0 220 1136 1400 1503
Size: 220 916 264 103
Fragments arranged by size:
916 264 220 103
MslI CAynn'nnrTG
Cuts at: 0 1190 1503
Size: 1190 313
MspA1I CmG'CkG
Cuts at: 0 398 449 1437 1503
Size: 398 51 988 66
Fragments arranged by size:
988 398 66 51
NcoI C'CATG_G
Cuts at: 0 438 1503
Size: 438 1065
NsiI A_TGCA'T
Cuts at: 0 410 1503
Size: 410 1093
NspI r_CATG'y
Cuts at: 0 408 462 833 1326 1503
Size: 408 54 371 493 177
Fragments arranged by size:
493 408 371 177 54
PasI CC'CwG_GG
Cuts at: 0 161 1503
Size: 161 1342
PflMI CCAn_nnn'nTGG
Cuts at: 0 1059 1503
Size: 1059 444
PfoI T'CCnGG_A
Cuts at: 0 145 302 1313 1343 1503
Size: 145 157 1011 30 160
Fragments arranged by size:
1011 160 157 145 30
PmlI CAC'GTG
Cuts at: 0 429 1503
Size: 429 1074
PpuMI rG'GwC_Cy
Cuts at: 0 321 1503
Size: 321 1182
PspOMI G'GGCC_C
Cuts at: 0 1439 1503
Size: 1439 64
PspXI vC'TCGA_Gb
Cuts at: 0 925 1503
Size: 925 578
PvuII CAG'CTG
Cuts at: 0 449 1503
Size: 449 1054
SacI G_AGCT'C
Cuts at: 0 245 1503
Size: 245 1258
SexAI A'CCwGG_T
Cuts at: 0 731 1031 1503
Size: 731 300 472
Fragments arranged by size:
731 472 300
SfcI C'TryA_G
Cuts at: 0 103 1503
Size: 103 1400
SgrAI Cr'CCGG_yG
Cuts at: 0 594 1503
Size: 594 909
SmlI C'TyrA_G
Cuts at: 0 841 925 1503
Size: 841 84 578
Fragments arranged by size:
841 578 84
SphI G_CATG'C
Cuts at: 0 408 833 1503
Size: 408 425 670
Fragments arranged by size:
670 425 408
StyI C'CwwG_G
Cuts at: 0 438 809 1503
Size: 438 371 694
Fragments arranged by size:
694 438 371
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 932 1503
Size: 73 859 571
Fragments arranged by size:
859 571 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 1503
Size: 193 32 1278
Fragments arranged by size:
1278 193 32
XhoI C'TCGA_G
Cuts at: 0 925 1503
Size: 925 578
XmnI GAAnn'nnTTC
Cuts at: 0 778 1503
Size: 778 725
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbvCI BcgI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PvuII SacI SexAI SfcI SgrAI SmlI SphI
StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BciVI BclI BmtI BplI BsaBI BsaHI
BsaWI BsiWI BspEI BspHI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PpiI PshAI PsiI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI Tth111I XbaI XcmI XmaI ZraI