Restriction map for
pFN21AE6420
(Linear) (Six-base) MAPSORT of: KIEE6420.seq Check: 7845 from: 1 to: 1521
KIEE6420.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AatII G_ACGT'C
Cuts at: 0 725 1521
Size: 725 796
AccI GT'mk_AC
Cuts at: 0 170 887 1521
Size: 170 717 634
Fragments arranged by size:
717 634 170
AfeI AGC'GCT
Cuts at: 0 202 1521
Size: 202 1319
AflIII A'CryG_T
Cuts at: 0 467 812 1521
Size: 467 345 709
Fragments arranged by size:
709 467 345
AleI CACnn'nnGTG
Cuts at: 0 1186 1521
Size: 1186 335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1521
Size: 1036 485
AlwNI CAG_nnn'CTG
Cuts at: 0 861 1158 1521
Size: 861 297 363
Fragments arranged by size:
861 363 297
ApaI G_GGCC'C
Cuts at: 0 31 807 1521
Size: 31 776 714
Fragments arranged by size:
776 714 31
AvaI C'yCGr_G
Cuts at: 0 49 668 1450 1521
Size: 49 619 782 71
Fragments arranged by size:
782 619 71 49
BamHI G'GATC_C
Cuts at: 0 210 538 1521
Size: 210 328 983
Fragments arranged by size:
983 328 210
BanI G'GyrC_C
Cuts at: 0 603 1188 1386 1521
Size: 603 585 198 135
Fragments arranged by size:
603 585 198 135
BanII G_rGCy'C
Cuts at: 0 31 443 504 807 1105 1307 1521
Size: 31 412 61 303 298 202 214
Fragments arranged by size:
412 303 298 214 202 61 31
BbeI G_GCGC'C
Cuts at: 0 607 1521
Size: 607 914
BbvCI CC'TCA_GC
Cuts at: 0 794 1521
Size: 794 727
BglI GCCn_nnn'nGGC
Cuts at: 0 601 694 1521
Size: 601 93 827
Fragments arranged by size:
827 601 93
BlpI GC'TnA_GC
Cuts at: 0 328 631 1521
Size: 328 303 890
Fragments arranged by size:
890 328 303
Bme1580I G_kGCm'C
Cuts at: 0 31 807 1193 1287 1451 1521
Size: 31 776 386 94 164 70
Fragments arranged by size:
776 386 164 94 70 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 744 1521
Size: 744 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1366 1492 1521
Size: 217 1149 126 29
Fragments arranged by size:
1149 217 126 29
Bpu10I CC'TnA_GC
Cuts at: 0 794 1521
Size: 794 727
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 1521
Size: 1447 74
BsaAI yAC'GTr
Cuts at: 0 468 813 1521
Size: 468 345 708
Fragments arranged by size:
708 468 345
BsaHI Gr'CG_yC
Cuts at: 0 604 722 1521
Size: 604 118 799
Fragments arranged by size:
799 604 118
BsaWI w'CCGG_w
Cuts at: 0 764 1521
Size: 764 757
BseYI C'CCAG_C
Cuts at: 0 15 189 518 1235 1521
Size: 15 174 329 717 286
Fragments arranged by size:
717 329 286 174 15
BsiEI CG_ry'CG
Cuts at: 0 281 362 1521
Size: 281 81 1159
Fragments arranged by size:
1159 281 81
BsiHKAI G_wGCw'C
Cuts at: 0 140 614 1372 1521
Size: 140 474 758 149
Fragments arranged by size:
758 474 149 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1035 1521
Size: 1035 486
Bsp1286I G_dGCh'C
Cuts at: 0 31 140 443 504 614 807 1105 1193
Size: 31 109 303 61 110 193 298 88
Cuts at: 1193 1287 1307 1372 1451 1521
Size: 94 20 65 79 70
Fragments arranged by size:
303 298 193 110 109 94 88 79
70 65 61 31 20
BspEI T'CCGG_A
Cuts at: 0 764 1521
Size: 764 757
BspHI T'CATG_A
Cuts at: 0 617 1521
Size: 617 904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 899 1521
Size: 227 672 622
Fragments arranged by size:
672 622 227
BsrFI r'CCGG_y
Cuts at: 0 361 818 1521
Size: 361 457 703
Fragments arranged by size:
703 457 361
BsrGI T'GTAC_A
Cuts at: 0 1073 1521
Size: 1073 448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1416 1521
Size: 831 585 105
Fragments arranged by size:
831 585 105
BstYI r'GATC_y
Cuts at: 0 210 538 749 1521
Size: 210 328 211 772
Fragments arranged by size:
772 328 211 210
Bsu36I CC'TnA_GG
Cuts at: 0 1299 1521
Size: 1299 222
BtgI C'CryG_G
Cuts at: 0 574 830 995 1229 1287 1521
Size: 574 256 165 234 58 234
Fragments arranged by size:
574 256 234 234 165 58
BtsI GCAGTG_nn'
Cuts at: 0 140 536 1112 1521
Size: 140 396 576 409
Fragments arranged by size:
576 409 396 140
DrdI GACnn_nn'nnGTC
Cuts at: 0 727 1521
Size: 727 794
EaeI y'GGCC_r
Cuts at: 0 119 278 359 593 1037 1199 1316 1406
Size: 119 159 81 234 444 162 117 90
Cuts at: 1406 1521
Size: 115
Fragments arranged by size:
444 234 162 159 119 117 115 90
81
EagI C'GGCC_G
Cuts at: 0 278 359 1521
Size: 278 81 1162
Fragments arranged by size:
1162 278 81
EarI CTCTTCn'nnn_
Cuts at: 0 677 1030 1216 1521
Size: 677 353 186 305
Fragments arranged by size:
677 353 305 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1366 1492 1521
Size: 217 1149 126 29
Fragments arranged by size:
1149 217 126 29
EcoO109I rG'GnC_Cy
Cuts at: 0 27 92 687 804 1240 1269 1508 1521
Size: 27 65 595 117 436 29 239 13
Fragments arranged by size:
595 436 239 117 65 29 27 13
FspI TGC'GCA
Cuts at: 0 386 1521
Size: 386 1135
FspAI rTGC'GCAy
Cuts at: 0 386 1521
Size: 386 1135
HaeII r_GCGC'y
Cuts at: 0 204 582 607 700 1521
Size: 204 378 25 93 821
Fragments arranged by size:
821 378 204 93 25
KasI G'GCGC_C
Cuts at: 0 603 1521
Size: 603 918
MscI TGG'CCA
Cuts at: 0 121 1201 1318 1521
Size: 121 1080 117 203
Fragments arranged by size:
1080 203 121 117
MslI CAynn'nnrTG
Cuts at: 0 460 529 829 1186 1521
Size: 460 69 300 357 335
Fragments arranged by size:
460 357 335 300 69
MspA1I CmG'CkG
Cuts at: 0 12 277 346 448 861 916 1036 1171
Size: 12 265 69 102 413 55 120 135
Cuts at: 1171 1322 1521
Size: 151 199
Fragments arranged by size:
413 265 199 151 135 120 102 69
55 12
NaeI GCC'GGC
Cuts at: 0 363 820 1521
Size: 363 457 701
Fragments arranged by size:
701 457 363
NarI GG'CG_CC
Cuts at: 0 604 1521
Size: 604 917
NcoI C'CATG_G
Cuts at: 0 995 1521
Size: 995 526
NgoMIV G'CCGG_C
Cuts at: 0 361 818 1521
Size: 361 457 703
Fragments arranged by size:
703 457 361
PasI CC'CwG_GG
Cuts at: 0 267 1521
Size: 267 1254
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 1250 1521
Size: 525 725 271
Fragments arranged by size:
725 525 271
PfoI T'CCnGG_A
Cuts at: 0 752 1521
Size: 752 769
PmlI CAC'GTG
Cuts at: 0 813 1521
Size: 813 708
PpuMI rG'GwC_Cy
Cuts at: 0 92 1240 1269 1508 1521
Size: 92 1148 29 239 13
Fragments arranged by size:
1148 239 92 29 13
PshAI GACnn'nnGTC
Cuts at: 0 721 1521
Size: 721 800
PspOMI G'GGCC_C
Cuts at: 0 27 803 1521
Size: 27 776 718
Fragments arranged by size:
776 718 27
PstI C_TGCA'G
Cuts at: 0 101 857 896 1521
Size: 101 756 39 625
Fragments arranged by size:
756 625 101 39
PvuII CAG'CTG
Cuts at: 0 346 448 861 1322 1521
Size: 346 102 413 461 199
Fragments arranged by size:
461 413 346 199 102
SanDI GG'GwC_CC
Cuts at: 0 92 1240 1521
Size: 92 1148 281
Fragments arranged by size:
1148 281 92
SapI GCTCTTCn'nnn_
Cuts at: 0 1030 1216 1521
Size: 1030 186 305
Fragments arranged by size:
1030 305 186
SfcI C'TryA_G
Cuts at: 0 97 853 892 1521
Size: 97 756 39 629
Fragments arranged by size:
756 629 97 39
SfoI GGC'GCC
Cuts at: 0 605 1521
Size: 605 916
StuI AGG'CCT
Cuts at: 0 287 1095 1521
Size: 287 808 426
Fragments arranged by size:
808 426 287
StyI C'CwwG_G
Cuts at: 0 206 558 932 995 1457 1521
Size: 206 352 374 63 462 64
Fragments arranged by size:
462 374 352 206 64 63
TatI w'GTAC_w
Cuts at: 0 1073 1521
Size: 1073 448
Tth111I GACn'n_nGTC
Cuts at: 0 760 1521
Size: 760 761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1521
Size: 1062 459
ZraI GAC'GTC
Cuts at: 0 723 1521
Size: 723 798
Enzymes that do cut:
AatII AccI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI Eco57MI EcoO109I FspI FspAI HaeII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SanDI SapI SfcI SfoI StuI StyI TatI
Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BpuEI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI XbaI XhoI XmaI
XmnI