Restriction map for
pFN21AE6419
(Linear) (Six-base) MAPSORT of: KIEE6419.seq Check: 1984 from: 1 to: 1488
KIEE6419.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
Acc65I G'GTAC_C
Cuts at: 0 212 1488
Size: 212 1276
AflII C'TTAA_G
Cuts at: 0 75 1488
Size: 75 1413
AflIII A'CryG_T
Cuts at: 0 1133 1488
Size: 1133 355
AhdI GACnn_n'nnGTC
Cuts at: 0 1293 1488
Size: 1293 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 775 1488
Size: 775 713
AlwNI CAG_nnn'CTG
Cuts at: 0 502 607 719 1488
Size: 502 105 112 769
Fragments arranged by size:
769 502 112 105
ApaI G_GGCC'C
Cuts at: 0 84 1362 1488
Size: 84 1278 126
Fragments arranged by size:
1278 126 84
ApoI r'AATT_y
Cuts at: 0 426 1488
Size: 426 1062
AseI AT'TA_AT
Cuts at: 0 731 1488
Size: 731 757
BamHI G'GATC_C
Cuts at: 0 1395 1488
Size: 1395 93
BanI G'GyrC_C
Cuts at: 0 42 212 1488
Size: 42 170 1276
Fragments arranged by size:
1276 170 42
BanII G_rGCy'C
Cuts at: 0 15 84 597 780 1362 1488
Size: 15 69 513 183 582 126
Fragments arranged by size:
582 513 183 126 69 15
BbeI G_GCGC'C
Cuts at: 0 46 1488
Size: 46 1442
BbvCI CC'TCA_GC
Cuts at: 0 329 1488
Size: 329 1159
BclI T'GATC_A
Cuts at: 0 171 1488
Size: 171 1317
BglI GCCn_nnn'nGGC
Cuts at: 0 79 909 1488
Size: 79 830 579
Fragments arranged by size:
830 579 79
BglII A'GATC_T
Cuts at: 0 1371 1488
Size: 1371 117
Bme1580I G_kGCm'C
Cuts at: 0 84 1362 1488
Size: 84 1278 126
Fragments arranged by size:
1278 126 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 1078 1488
Size: 1078 410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 1488
Size: 400 1088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1488
Size: 794 694
Bpu10I CC'TnA_GC
Cuts at: 0 329 1488
Size: 329 1159
BsaI GGTCTCn'nnnn_
Cuts at: 0 1194 1488
Size: 1194 294
BsaHI Gr'CG_yC
Cuts at: 0 43 1488
Size: 43 1445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1292 1322 1488
Size: 1292 30 166
Fragments arranged by size:
1292 166 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1074 1488
Size: 1074 414
BsiEI CG_ry'CG
Cuts at: 0 70 1488
Size: 70 1418
BsiHKAI G_wGCw'C
Cuts at: 0 597 1488
Size: 597 891
BsmI GAATG_Cn'
Cuts at: 0 1392 1488
Size: 1392 96
Bsp1286I G_dGCh'C
Cuts at: 0 15 84 597 780 1362 1488
Size: 15 69 513 183 582 126
Fragments arranged by size:
582 513 183 126 69 15
BsrBI CCG'CTC
Cuts at: 0 1421 1488
Size: 1421 67
BsrDI GCAATG_nn'
Cuts at: 0 518 866 1281 1488
Size: 518 348 415 207
Fragments arranged by size:
518 415 348 207
BsrFI r'CCGG_y
Cuts at: 0 69 1488
Size: 69 1419
BstAPI GCAn_nnn'nTGC
Cuts at: 0 607 1488
Size: 607 881
BstYI r'GATC_y
Cuts at: 0 833 1371 1395 1488
Size: 833 538 24 93
Fragments arranged by size:
833 538 93 24
BtsI GCAGTG_nn'
Cuts at: 0 759 769 961 1488
Size: 759 10 192 527
Fragments arranged by size:
759 527 192 10
ClaI AT'CG_AT
Cuts at: 0 366 1488
Size: 366 1122
EaeI y'GGCC_r
Cuts at: 0 67 1488
Size: 67 1421
EagI C'GGCC_G
Cuts at: 0 67 1488
Size: 67 1421
EcoICRI GAG'CTC
Cuts at: 0 595 1488
Size: 595 893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1488
Size: 794 694
EcoNI CCTnn'n_nnAGG
Cuts at: 0 561 1488
Size: 561 927
EcoO109I rG'GnC_Cy
Cuts at: 0 80 81 716 1208 1358 1488
Size: 80 1 635 492 150 130
Fragments arranged by size:
635 492 150 130 80 1
EcoRV GAT'ATC
Cuts at: 0 1320 1488
Size: 1320 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1407 1488
Size: 1407 81
FseI GG_CCGG'CC
Cuts at: 0 73 1488
Size: 73 1415
HaeII r_GCGC'y
Cuts at: 0 46 1488
Size: 46 1442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 820 852 1279 1311 1387 1419
Size: 558 32 230 32 427 32 76 32
Cuts at: 1419 1488
Size: 69
Fragments arranged by size:
558 427 230 76 69 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 42 1488
Size: 42 1446
KpnI G_GTAC'C
Cuts at: 0 216 1488
Size: 216 1272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 570 840 1054 1488
Size: 183 387 270 214 434
Fragments arranged by size:
434 387 270 214 183
MslI CAynn'nnrTG
Cuts at: 0 1225 1488
Size: 1225 263
MspA1I CmG'CkG
Cuts at: 0 210 1488
Size: 210 1278
NaeI GCC'GGC
Cuts at: 0 71 1488
Size: 71 1417
NarI GG'CG_CC
Cuts at: 0 43 1488
Size: 43 1445
NdeI CA'TA_TG
Cuts at: 0 581 1439 1488
Size: 581 858 49
Fragments arranged by size:
858 581 49
NgoMIV G'CCGG_C
Cuts at: 0 69 1488
Size: 69 1419
NspI r_CATG'y
Cuts at: 0 549 1137 1488
Size: 549 588 351
Fragments arranged by size:
588 549 351
PasI CC'CwG_GG
Cuts at: 0 47 1488
Size: 47 1441
PciI A'CATG_T
Cuts at: 0 1133 1488
Size: 1133 355
PfoI T'CCnGG_A
Cuts at: 0 1213 1488
Size: 1213 275
PpuMI rG'GwC_Cy
Cuts at: 0 716 1488
Size: 716 772
PspOMI G'GGCC_C
Cuts at: 0 80 1358 1488
Size: 80 1278 130
Fragments arranged by size:
1278 130 80
PstI C_TGCA'G
Cuts at: 0 974 1488
Size: 974 514
SacI G_AGCT'C
Cuts at: 0 597 1488
Size: 597 891
SfcI C'TryA_G
Cuts at: 0 128 668 970 1488
Size: 128 540 302 518
Fragments arranged by size:
540 518 302 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 79 1488
Size: 79 1409
SfoI GGC'GCC
Cuts at: 0 44 1488
Size: 44 1444
SmlI C'TyrA_G
Cuts at: 0 75 1488
Size: 75 1413
SphI G_CATG'C
Cuts at: 0 549 1488
Size: 549 939
SspI AAT'ATT
Cuts at: 0 978 1035 1488
Size: 978 57 453
Fragments arranged by size:
978 453 57
StuI AGG'CCT
Cuts at: 0 1087 1488
Size: 1087 401
StyI C'CwwG_G
Cuts at: 0 1204 1391 1424 1488
Size: 1204 187 33 64
Fragments arranged by size:
1204 187 64 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 1488
Size: 837 651
TatI w'GTAC_w
Cuts at: 0 1114 1488
Size: 1114 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1211 1389 1488
Size: 1211 178 99
Fragments arranged by size:
1211 178 99
XmnI GAAnn'nnTTC
Cuts at: 0 979 1488
Size: 979 509
Enzymes that do cut:
Acc65I AflII AflIII AhdI AlfI AlwNI ApaI ApoI
AseI BamHI BanI BanII BbeI BbvCI BclI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI
BsaXI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI
BsrFI BstAPI BstYI BtsI ClaI EaeI EagI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PasI PciI PfoI PpuMI PspOMI PstI
SacI SfcI SfiI SfoI SmlI SphI SspI StuI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AgeI AleI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BbsI
BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaAI
BsaBI BsaWI BseRI BseYI BsiWI BsmBI BspEI BspHI
BspMI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EarI
EciI EcoRI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI NcoI NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI