Restriction map for
pFN21AE6416
(Linear) (Six-base) MAPSORT of: KIEE6416.seq Check: 5506 from: 1 to: 1647
KIEE6416.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 395 1647
Size: 395 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 318 339 1647
Size: 235 83 21 1308
Fragments arranged by size:
1308 235 83 21
AleI CACnn'nnGTG
Cuts at: 0 481 1647
Size: 481 1166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 1647
Size: 241 1406
AlwNI CAG_nnn'CTG
Cuts at: 0 985 1647
Size: 985 662
ApaI G_GGCC'C
Cuts at: 0 378 1647
Size: 378 1269
ApoI r'AATT_y
Cuts at: 0 51 559 1591 1647
Size: 51 508 1032 56
Fragments arranged by size:
1032 508 56 51
BanII G_rGCy'C
Cuts at: 0 378 1647
Size: 378 1269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 511 545 1647
Size: 511 34 1102
Fragments arranged by size:
1102 511 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 156 1647
Size: 156 1491
BglI GCCn_nnn'nGGC
Cuts at: 0 1042 1647
Size: 1042 605
Bme1580I G_kGCm'C
Cuts at: 0 378 1647
Size: 378 1269
BmgBI CAC'GTC
Cuts at: 0 525 1647
Size: 525 1122
BmrI ACTGGGnnnn_n'
Cuts at: 0 1504 1647
Size: 1504 143
BsaAI yAC'GTr
Cuts at: 0 246 1647
Size: 246 1401
BseYI C'CCAG_C
Cuts at: 0 1109 1647
Size: 1109 538
BsiEI CG_ry'CG
Cuts at: 0 414 1647
Size: 414 1233
Bsp1286I G_dGCh'C
Cuts at: 0 378 1647
Size: 378 1269
BspHI T'CATG_A
Cuts at: 0 461 1647
Size: 461 1186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1093 1647
Size: 1093 554
BsrBI CCG'CTC
Cuts at: 0 80 1647
Size: 80 1567
BsrDI GCAATG_nn'
Cuts at: 0 581 690 1647
Size: 581 109 957
Fragments arranged by size:
957 581 109
BsrFI r'CCGG_y
Cuts at: 0 32 166 1647
Size: 32 134 1481
Fragments arranged by size:
1481 134 32
BsrGI T'GTAC_A
Cuts at: 0 1274 1647
Size: 1274 373
BstAPI GCAn_nnn'nTGC
Cuts at: 0 982 1647
Size: 982 665
BstXI CCAn_nnnn'nTGG
Cuts at: 0 101 483 1647
Size: 101 382 1164
Fragments arranged by size:
1164 382 101
BstYI r'GATC_y
Cuts at: 0 1413 1647
Size: 1413 234
BtsI GCAGTG_nn'
Cuts at: 0 1097 1647
Size: 1097 550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 275 310 1647
Size: 275 35 1337
Fragments arranged by size:
1337 275 35
DraI TTT'AAA
Cuts at: 0 1355 1596 1647
Size: 1355 241 51
Fragments arranged by size:
1355 241 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 586 1647
Size: 586 1061
EaeI y'GGCC_r
Cuts at: 0 43 566 1647
Size: 43 523 1081
Fragments arranged by size:
1081 523 43
EarI CTCTTCn'nnn_
Cuts at: 0 70 158 463 1004 1647
Size: 70 88 305 541 643
Fragments arranged by size:
643 541 305 88 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 318 339 1647
Size: 235 83 21 1308
Fragments arranged by size:
1308 235 83 21
EcoO109I rG'GnC_Cy
Cuts at: 0 375 1105 1647
Size: 375 730 542
Fragments arranged by size:
730 542 375
EcoRI G'AATT_C
Cuts at: 0 559 1647
Size: 559 1088
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1600 1632 1647
Size: 1600 32 15
Fragments arranged by size:
1600 32 15
HincII GTy'rAC
Cuts at: 0 605 1647
Size: 605 1042
HindIII A'AGCT_T
Cuts at: 0 326 1647
Size: 326 1321
HpaI GTT'AAC
Cuts at: 0 605 1647
Size: 605 1042
MslI CAynn'nnrTG
Cuts at: 0 481 1623 1647
Size: 481 1142 24
Fragments arranged by size:
1142 481 24
MspA1I CmG'CkG
Cuts at: 0 127 667 1647
Size: 127 540 980
Fragments arranged by size:
980 540 127
NaeI GCC'GGC
Cuts at: 0 34 1647
Size: 34 1613
NgoMIV G'CCGG_C
Cuts at: 0 32 1647
Size: 32 1615
NspI r_CATG'y
Cuts at: 0 1284 1647
Size: 1284 363
PasI CC'CwG_GG
Cuts at: 0 264 1647
Size: 264 1383
PflMI CCAn_nnn'nTGG
Cuts at: 0 1330 1647
Size: 1330 317
PpuMI rG'GwC_Cy
Cuts at: 0 1105 1647
Size: 1105 542
PsiI TTA'TAA
Cuts at: 0 913 1647
Size: 913 734
PspOMI G'GGCC_C
Cuts at: 0 374 1647
Size: 374 1273
PstI C_TGCA'G
Cuts at: 0 1104 1647
Size: 1104 543
SapI GCTCTTCn'nnn_
Cuts at: 0 70 158 1647
Size: 70 88 1489
Fragments arranged by size:
1489 88 70
SfcI C'TryA_G
Cuts at: 0 1100 1143 1391 1647
Size: 1100 43 248 256
Fragments arranged by size:
1100 256 248 43
SspI AAT'ATT
Cuts at: 0 730 1647
Size: 730 917
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 400 1486 1647
Size: 21 379 1086 161
Fragments arranged by size:
1086 379 161 21
TatI w'GTAC_w
Cuts at: 0 1274 1647
Size: 1274 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 185 217 1089 1121 1647
Size: 185 32 872 32 526
Fragments arranged by size:
872 526 185 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 624 1647
Size: 624 1023
XmnI GAAnn'nnTTC
Cuts at: 0 152 1647
Size: 152 1495
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApoI BanII
BcgI BciVI BglI Bme1580I BmgBI BmrI BsaAI BseYI
BsiEI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BtsI CspCI DraI DrdI EaeI
EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI
MslI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI
PsiI PspOMI PstI SapI SfcI SspI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BclI BfrBI
BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI DraIII EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI
KpnI MfeI MluI MmeI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI SwaI Tth111I XbaI XhoI XmaI
ZraI