Restriction map for
pFN21AE6409
(Linear) (Six-base) MAPSORT of: KIEE6409.seq Check: 8261 from: 1 to: 1413
KIEE6409.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 873 1413
Size: 873 540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 122 245 490 695 1413
Size: 73 49 123 245 205 718
Fragments arranged by size:
718 245 205 123 73 49
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 754 1413
Size: 754 659
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 1413
Size: 685 32 696
Fragments arranged by size:
696 685 32
ApoI r'AATT_y
Cuts at: 0 289 1413
Size: 289 1124
AvrII C'CTAG_G
Cuts at: 0 1122 1413
Size: 1122 291
BanI G'GyrC_C
Cuts at: 0 649 1413
Size: 649 764
BanII G_rGCy'C
Cuts at: 0 83 917 1316 1413
Size: 83 834 399 97
Fragments arranged by size:
834 399 97 83
Bpu10I CC'TnA_GC
Cuts at: 0 623 1413
Size: 623 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 832 1413
Size: 97 735 581
Fragments arranged by size:
735 581 97
BsaI GGTCTCn'nnnn_
Cuts at: 0 1053 1114 1413
Size: 1053 61 299
Fragments arranged by size:
1053 299 61
BsaBI GATnn'nnATC
Cuts at: 0 683 1413
Size: 683 730
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1413
Size: 17 30 1366
Fragments arranged by size:
1366 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 128 970 1413
Size: 128 842 443
Fragments arranged by size:
842 443 128
BseYI C'CCAG_C
Cuts at: 0 28 1413
Size: 28 1385
BsiHKAI G_wGCw'C
Cuts at: 0 917 1316 1413
Size: 917 399 97
Fragments arranged by size:
917 399 97
BsmI GAATG_Cn'
Cuts at: 0 621 1413
Size: 621 792
Bsp1286I G_dGCh'C
Cuts at: 0 83 917 1316 1413
Size: 83 834 399 97
Fragments arranged by size:
834 399 97 83
BspHI T'CATG_A
Cuts at: 0 659 1413
Size: 659 754
BsrBI CCG'CTC
Cuts at: 0 1075 1413
Size: 1075 338
BsrDI GCAATG_nn'
Cuts at: 0 738 1413
Size: 738 675
BstYI r'GATC_y
Cuts at: 0 1365 1413
Size: 1365 48
BtsI GCAGTG_nn'
Cuts at: 0 444 1413
Size: 444 969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1413
Size: 72 35 1306
Fragments arranged by size:
1306 72 35
EaeI y'GGCC_r
Cuts at: 0 147 366 441 1413
Size: 147 219 75 972
Fragments arranged by size:
972 219 147 75
EcoICRI GAG'CTC
Cuts at: 0 915 1314 1413
Size: 915 399 99
Fragments arranged by size:
915 399 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 122 245 490 695 1413
Size: 73 49 123 245 205 718
Fragments arranged by size:
718 245 205 123 73 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 143 175 330 343 362 375
Size: 17 32 94 32 155 13 19 13
Cuts at: 375 1333 1365 1413
Size: 958 32 48
Fragments arranged by size:
958 155 94 48 32 32 32 19
17 13 13
HincII GTy'rAC
Cuts at: 0 1091 1299 1413
Size: 1091 208 114
Fragments arranged by size:
1091 208 114
MscI TGG'CCA
Cuts at: 0 368 443 1413
Size: 368 75 970
Fragments arranged by size:
970 368 75
MslI CAynn'nnrTG
Cuts at: 0 536 562 892 1413
Size: 536 26 330 521
Fragments arranged by size:
536 521 330 26
MspA1I CmG'CkG
Cuts at: 0 751 1413
Size: 751 662
PshAI GACnn'nnGTC
Cuts at: 0 325 1413
Size: 325 1088
PvuII CAG'CTG
Cuts at: 0 751 1413
Size: 751 662
SacI G_AGCT'C
Cuts at: 0 917 1316 1413
Size: 917 399 97
Fragments arranged by size:
917 399 97
SfcI C'TryA_G
Cuts at: 0 902 1413
Size: 902 511
SmlI C'TyrA_G
Cuts at: 0 76 811 1413
Size: 76 735 602
Fragments arranged by size:
735 602 76
SspI AAT'ATT
Cuts at: 0 268 508 1413
Size: 268 240 905
Fragments arranged by size:
905 268 240
StyI C'CwwG_G
Cuts at: 0 688 1122 1413
Size: 688 434 291
Fragments arranged by size:
688 434 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1152 1413
Size: 1152 261
TatI w'GTAC_w
Cuts at: 0 735 846 1413
Size: 735 111 567
Fragments arranged by size:
735 567 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 802 1413
Size: 802 611
XmnI GAAnn'nnTTC
Cuts at: 0 230 1413
Size: 230 1183
Enzymes that do cut:
AccI AcuI AlfI AloI ApoI AvrII BanI BanII
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI BstYI BtsI CspCI
EaeI EcoICRI Eco57MI Hin4I HincII MscI MslI MspA1I
PshAI PvuII SacI SfcI SmlI SspI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BsaAI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI