Restriction map for
pFN21AE6404
(Linear) (Six-base) MAPSORT of: KIEE6404.seq Check: 1762 from: 1 to: 1362
KIEE6404.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 788 1362
Size: 788 574
AccI GT'mk_AC
Cuts at: 0 1175 1362
Size: 1175 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1362
Size: 385 977
AfeI AGC'GCT
Cuts at: 0 1107 1362
Size: 1107 255
AflIII A'CryG_T
Cuts at: 0 1196 1362
Size: 1196 166
AleI CACnn'nnGTG
Cuts at: 0 142 1219 1362
Size: 142 1077 143
Fragments arranged by size:
1077 143 142
AlwNI CAG_nnn'CTG
Cuts at: 0 363 576 780 1362
Size: 363 213 204 582
Fragments arranged by size:
582 363 213 204
AvaI C'yCGr_G
Cuts at: 0 170 912 1063 1271 1362
Size: 170 742 151 208 91
Fragments arranged by size:
742 208 170 151 91
BanI G'GyrC_C
Cuts at: 0 502 916 1362
Size: 502 414 446
Fragments arranged by size:
502 446 414
BanII G_rGCy'C
Cuts at: 0 400 995 1362
Size: 400 595 367
Fragments arranged by size:
595 400 367
BbeI G_GCGC'C
Cuts at: 0 920 1362
Size: 920 442
BbvCI CC'TCA_GC
Cuts at: 0 105 1362
Size: 105 1257
BfrBI ATG'CAT
Cuts at: 0 131 1362
Size: 131 1231
BglI GCCn_nnn'nGGC
Cuts at: 0 547 914 1362
Size: 547 367 448
Fragments arranged by size:
547 448 367
Bme1580I G_kGCm'C
Cuts at: 0 805 1226 1362
Size: 805 421 136
Fragments arranged by size:
805 421 136
BmgBI CAC'GTC
Cuts at: 0 1199 1221 1338 1362
Size: 1199 22 117 24
Fragments arranged by size:
1199 117 24 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 665 1362
Size: 222 443 697
Fragments arranged by size:
697 443 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 427 565 748 1362
Size: 253 174 138 183 614
Fragments arranged by size:
614 253 183 174 138
Bpu10I CC'TnA_GC
Cuts at: 0 45 105 394 1362
Size: 45 60 289 968
Fragments arranged by size:
968 289 60 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1362
Size: 265 1097
BsaI GGTCTCn'nnnn_
Cuts at: 0 283 1362
Size: 283 1079
BsaAI yAC'GTr
Cuts at: 0 813 1143 1362
Size: 813 330 219
Fragments arranged by size:
813 330 219
BsaHI Gr'CG_yC
Cuts at: 0 917 1362
Size: 917 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 811 841 1362
Size: 811 30 521
Fragments arranged by size:
811 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 508 918 1362
Size: 466 42 410 444
Fragments arranged by size:
466 444 410 42
BseYI C'CCAG_C
Cuts at: 0 150 576 788 1162 1362
Size: 150 426 212 374 200
Fragments arranged by size:
426 374 212 200 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 919 1362
Size: 120 799 443
Fragments arranged by size:
799 443 120
BsiEI CG_ry'CG
Cuts at: 0 143 1362
Size: 143 1219
BsiHKAI G_wGCw'C
Cuts at: 0 46 400 1139 1316 1362
Size: 46 354 739 177 46
Fragments arranged by size:
739 354 177 46 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 228 1362
Size: 228 1134
Bsp1286I G_dGCh'C
Cuts at: 0 46 400 805 995 1139 1226 1316 1362
Size: 46 354 405 190 144 87 90 46
Fragments arranged by size:
405 354 190 144 90 87 46 46
BspHI T'CATG_A
Cuts at: 0 1121 1362
Size: 1121 241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 172 530 788 1362
Size: 172 358 258 574
Fragments arranged by size:
574 358 258 172
BsrBI CCG'CTC
Cuts at: 0 9 881 989 1362
Size: 9 872 108 373
Fragments arranged by size:
872 373 108 9
BsrFI r'CCGG_y
Cuts at: 0 919 1043 1087 1362
Size: 919 124 44 275
Fragments arranged by size:
919 275 124 44
BsrGI T'GTAC_A
Cuts at: 0 98 818 1004 1362
Size: 98 720 186 358
Fragments arranged by size:
720 358 186 98
BssHII G'CGCG_C
Cuts at: 0 1278 1362
Size: 1278 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 783 1362
Size: 783 579
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1162 1362
Size: 1162 200
BtgI C'CryG_G
Cuts at: 0 233 626 793 1362
Size: 233 393 167 569
Fragments arranged by size:
569 393 233 167
DraIII CAC_nnn'GTG
Cuts at: 0 816 1311 1362
Size: 816 495 51
Fragments arranged by size:
816 495 51
EaeI y'GGCC_r
Cuts at: 0 4 53 140 344 482 1302 1362
Size: 4 49 87 204 138 820 60
Fragments arranged by size:
820 204 138 87 60 49 4
EagI C'GGCC_G
Cuts at: 0 140 1362
Size: 140 1222
EarI CTCTTCn'nnn_
Cuts at: 0 73 77 1362
Size: 73 4 1285
Fragments arranged by size:
1285 73 4
EcoICRI GAG'CTC
Cuts at: 0 398 1362
Size: 398 964
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 385 427 565 748 1362
Size: 253 132 42 138 183 614
Fragments arranged by size:
614 253 183 138 132 42
EcoO109I rG'GnC_Cy
Cuts at: 0 114 240 335 519 632 723 1356 1362
Size: 114 126 95 184 113 91 633 6
Fragments arranged by size:
633 184 126 114 113 95 91 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1194 1362
Size: 1194 168
HaeII r_GCGC'y
Cuts at: 0 920 1109 1362
Size: 920 189 253
Fragments arranged by size:
920 253 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 50 82 737 769 1362
Size: 50 32 655 32 593
Fragments arranged by size:
655 593 50 32 32
KasI G'GCGC_C
Cuts at: 0 916 1362
Size: 916 446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 1362
Size: 986 376
MslI CAynn'nnrTG
Cuts at: 0 142 1219 1252 1362
Size: 142 1077 33 110
Fragments arranged by size:
1077 142 110 33
MspA1I CmG'CkG
Cuts at: 0 27 96 328 1362
Size: 27 69 232 1034
Fragments arranged by size:
1034 232 69 27
NaeI GCC'GGC
Cuts at: 0 1045 1362
Size: 1045 317
NarI GG'CG_CC
Cuts at: 0 917 1362
Size: 917 445
NcoI C'CATG_G
Cuts at: 0 233 626 1362
Size: 233 393 736
Fragments arranged by size:
736 393 233
NgoMIV G'CCGG_C
Cuts at: 0 1043 1362
Size: 1043 319
NruI TCG'CGA
Cuts at: 0 263 1362
Size: 263 1099
NsiI A_TGCA'T
Cuts at: 0 133 1362
Size: 133 1229
PasI CC'CwG_GG
Cuts at: 0 741 1362
Size: 741 621
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 576 1362
Size: 234 342 786
Fragments arranged by size:
786 342 234
PfoI T'CCnGG_A
Cuts at: 0 356 404 646 725 1362
Size: 356 48 242 79 637
Fragments arranged by size:
637 356 242 79 48
PmlI CAC'GTG
Cuts at: 0 813 1362
Size: 813 549
PpuMI rG'GwC_Cy
Cuts at: 0 240 335 519 632 723 1356 1362
Size: 240 95 184 113 91 633 6
Fragments arranged by size:
633 240 184 113 95 91 6
PshAI GACnn'nnGTC
Cuts at: 0 598 1362
Size: 598 764
PspXI vC'TCGA_Gb
Cuts at: 0 1063 1362
Size: 1063 299
PstI C_TGCA'G
Cuts at: 0 527 1362
Size: 527 835
PvuII CAG'CTG
Cuts at: 0 96 328 1362
Size: 96 232 1034
Fragments arranged by size:
1034 232 96
SacI G_AGCT'C
Cuts at: 0 400 1362
Size: 400 962
SanDI GG'GwC_CC
Cuts at: 0 335 632 1356 1362
Size: 335 297 724 6
Fragments arranged by size:
724 335 297 6
SexAI A'CCwGG_T
Cuts at: 0 1307 1362
Size: 1307 55
SfcI C'TryA_G
Cuts at: 0 523 1362
Size: 523 839
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 547 1362
Size: 547 815
SfoI GGC'GCC
Cuts at: 0 918 1362
Size: 918 444
SmaI CCC'GGG
Cuts at: 0 914 1273 1362
Size: 914 359 89
Fragments arranged by size:
914 359 89
SmlI C'TyrA_G
Cuts at: 0 170 244 1063 1362
Size: 170 74 819 299
Fragments arranged by size:
819 299 170 74
StuI AGG'CCT
Cuts at: 0 50 1117 1362
Size: 50 1067 245
Fragments arranged by size:
1067 245 50
StyI C'CwwG_G
Cuts at: 0 233 626 1169 1362
Size: 233 393 543 193
Fragments arranged by size:
543 393 233 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 390 490 1362
Size: 390 100 872
Fragments arranged by size:
872 390 100
TatI w'GTAC_w
Cuts at: 0 98 413 818 1004 1211 1362
Size: 98 315 405 186 207 151
Fragments arranged by size:
405 315 207 186 151 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 86 1155 1362
Size: 86 1069 207
Fragments arranged by size:
1069 207 86
XhoI C'TCGA_G
Cuts at: 0 170 1063 1362
Size: 170 893 299
Fragments arranged by size:
893 299 170
XmaI C'CCGG_G
Cuts at: 0 912 1271 1362
Size: 912 359 91
Fragments arranged by size:
912 359 91
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI AvaI
BanI BanII BbeI BbvCI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BtgI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI PasI PflMI PfoI PmlI
PpuMI PshAI PspXI PstI PvuII SacI SanDI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BglII BlpI BmtI
BplI BsaBI BsaWI BsiWI BsmI BspEI BsrDI BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NspI PacI PciI PmeI
PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XmnI ZraI