Restriction map for
pFN21AE6403
(Linear) (Six-base) MAPSORT of: KIEE6403.seq Check: 5827 from: 1 to: 1533
KIEE6403.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 1533
Size: 78 1455
Acc65I G'GTAC_C
Cuts at: 0 1442 1533
Size: 1442 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 767 1143 1533
Size: 118 649 376 390
Fragments arranged by size:
649 390 376 118
AleI CACnn'nnGTG
Cuts at: 0 1072 1533
Size: 1072 461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 1533
Size: 814 719
AlwNI CAG_nnn'CTG
Cuts at: 0 359 1166 1455 1471 1533
Size: 359 807 289 16 62
Fragments arranged by size:
807 359 289 62 16
ApaI G_GGCC'C
Cuts at: 0 261 1533
Size: 261 1272
ApoI r'AATT_y
Cuts at: 0 645 1533
Size: 645 888
AvaI C'yCGr_G
Cuts at: 0 156 444 1533
Size: 156 288 1089
Fragments arranged by size:
1089 288 156
BamHI G'GATC_C
Cuts at: 0 1518 1533
Size: 1518 15
BanI G'GyrC_C
Cuts at: 0 280 1442 1533
Size: 280 1162 91
Fragments arranged by size:
1162 280 91
BanII G_rGCy'C
Cuts at: 0 261 386 1015 1533
Size: 261 125 629 518
Fragments arranged by size:
629 518 261 125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1133 1260 1533
Size: 1133 127 273
Fragments arranged by size:
1133 273 127
BglII A'GATC_T
Cuts at: 0 107 1533
Size: 107 1426
BlpI GC'TnA_GC
Cuts at: 0 1246 1533
Size: 1246 287
Bme1580I G_kGCm'C
Cuts at: 0 261 283 1533
Size: 261 22 1250
Fragments arranged by size:
1250 261 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1533
Size: 286 1247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1432 1533
Size: 343 1089 101
Fragments arranged by size:
1089 343 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1225 1533
Size: 999 226 308
Fragments arranged by size:
999 308 226
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 1533
Size: 214 1319
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 1533
Size: 184 30 1319
Fragments arranged by size:
1319 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1511 1533
Size: 1511 22
BseYI C'CCAG_C
Cuts at: 0 32 722 1533
Size: 32 690 811
Fragments arranged by size:
811 690 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1263 1533
Size: 1263 270
BsiEI CG_ry'CG
Cuts at: 0 1405 1533
Size: 1405 128
BsiHKAI G_wGCw'C
Cuts at: 0 188 1533
Size: 188 1345
Bsp1286I G_dGCh'C
Cuts at: 0 188 261 283 386 1015 1533
Size: 188 73 22 103 629 518
Fragments arranged by size:
629 518 188 103 73 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 782 1533
Size: 78 704 751
Fragments arranged by size:
751 704 78
BsrBI CCG'CTC
Cuts at: 0 1062 1533
Size: 1062 471
BsrDI GCAATG_nn'
Cuts at: 0 401 680 1337 1343 1533
Size: 401 279 657 6 190
Fragments arranged by size:
657 401 279 190 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 1290 1533
Size: 426 864 243
Fragments arranged by size:
864 426 243
BstYI r'GATC_y
Cuts at: 0 107 1518 1533
Size: 107 1411 15
Fragments arranged by size:
1411 107 15
Bsu36I CC'TnA_GG
Cuts at: 0 38 1533
Size: 38 1495
BtsI GCAGTG_nn'
Cuts at: 0 112 677 1533
Size: 112 565 856
Fragments arranged by size:
856 565 112
EaeI y'GGCC_r
Cuts at: 0 618 872 1533
Size: 618 254 661
Fragments arranged by size:
661 618 254
EarI CTCTTCn'nnn_
Cuts at: 0 247 445 743 1093 1533
Size: 247 198 298 350 440
Fragments arranged by size:
440 350 298 247 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 343 767 1143 1432 1533
Size: 118 225 424 376 289 101
Fragments arranged by size:
424 376 289 225 118 101
EcoO109I rG'GnC_Cy
Cuts at: 0 237 257 258 1452 1533
Size: 237 20 1 1194 81
Fragments arranged by size:
1194 237 81 20 1
EcoRI G'AATT_C
Cuts at: 0 645 1533
Size: 645 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 744 776 1510 1533
Size: 646 32 66 32 734 23
Fragments arranged by size:
734 646 66 32 32 23
HincII GTy'rAC
Cuts at: 0 6 1533
Size: 6 1527
KpnI G_GTAC'C
Cuts at: 0 1446 1533
Size: 1446 87
MscI TGG'CCA
Cuts at: 0 620 874 1533
Size: 620 254 659
Fragments arranged by size:
659 620 254
MslI CAynn'nnrTG
Cuts at: 0 796 1045 1072 1384 1533
Size: 796 249 27 312 149
Fragments arranged by size:
796 312 249 149 27
PasI CC'CwG_GG
Cuts at: 0 76 1533
Size: 76 1457
PfoI T'CCnGG_A
Cuts at: 0 320 1533
Size: 320 1213
PpuMI rG'GwC_Cy
Cuts at: 0 1452 1533
Size: 1452 81
PspOMI G'GGCC_C
Cuts at: 0 257 1533
Size: 257 1276
PstI C_TGCA'G
Cuts at: 0 1476 1533
Size: 1476 57
SapI GCTCTTCn'nnn_
Cuts at: 0 1093 1533
Size: 1093 440
SfcI C'TryA_G
Cuts at: 0 1152 1472 1533
Size: 1152 320 61
Fragments arranged by size:
1152 320 61
SmlI C'TyrA_G
Cuts at: 0 1014 1240 1533
Size: 1014 226 293
Fragments arranged by size:
1014 293 226
SspI AAT'ATT
Cuts at: 0 920 1533
Size: 920 613
StuI AGG'CCT
Cuts at: 0 328 1358 1533
Size: 328 1030 175
Fragments arranged by size:
1030 328 175
StyI C'CwwG_G
Cuts at: 0 176 1533
Size: 176 1357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 664 1533
Size: 49 615 869
Fragments arranged by size:
869 615 49
TatI w'GTAC_w
Cuts at: 0 695 1533
Size: 695 838
Tth111I GACn'n_nGTC
Cuts at: 0 1232 1533
Size: 1232 301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 1290 1533
Size: 477 813 243
Fragments arranged by size:
813 477 243
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BglII BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI
Bsu36I BtsI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I
HincII KpnI MscI MslI PasI PfoI PpuMI PspOMI
PstI SapI SfcI SmlI SspI StuI StyI TaqII
TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI TstI XbaI XhoI XmaI XmnI
ZraI