Restriction map for
pFN21AE6402
(Linear) (Six-base) MAPSORT of: KIEE6402.seq Check: 8628 from: 1 to: 1605
KIEE6402.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1584 1605
Size: 1584 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 272 409 422 1455 1605
Size: 89 183 137 13 1033 150
Fragments arranged by size:
1033 183 150 137 89 13
AfeI AGC'GCT
Cuts at: 0 946 1605
Size: 946 659
ApoI r'AATT_y
Cuts at: 0 139 735 1605
Size: 139 596 870
Fragments arranged by size:
870 596 139
BamHI G'GATC_C
Cuts at: 0 38 781 1605
Size: 38 743 824
Fragments arranged by size:
824 743 38
BanI G'GyrC_C
Cuts at: 0 685 1605
Size: 685 920
BanII G_rGCy'C
Cuts at: 0 36 1605
Size: 36 1569
BbsI GAAGACnn'nnnn_
Cuts at: 0 391 1605
Size: 391 1214
BclI T'GATC_A
Cuts at: 0 1073 1605
Size: 1073 532
BglI GCCn_nnn'nGGC
Cuts at: 0 226 1605
Size: 226 1379
Bme1580I G_kGCm'C
Cuts at: 0 690 1605
Size: 690 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 665 1073 1297 1605
Size: 658 7 408 224 308
Fragments arranged by size:
658 408 308 224 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 466 1605
Size: 466 1139
BsaBI GATnn'nnATC
Cuts at: 0 172 1605
Size: 172 1433
BsaWI w'CCGG_w
Cuts at: 0 753 1605
Size: 753 852
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1400 1430 1605
Size: 1400 30 175
Fragments arranged by size:
1400 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1261 1605
Size: 1261 344
BseYI C'CCAG_C
Cuts at: 0 183 1605
Size: 183 1422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1605
Size: 557 1048
Bsp1286I G_dGCh'C
Cuts at: 0 36 690 1605
Size: 36 654 915
Fragments arranged by size:
915 654 36
BspEI T'CCGG_A
Cuts at: 0 753 1605
Size: 753 852
BspHI T'CATG_A
Cuts at: 0 461 1605
Size: 461 1144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 297 1584 1605
Size: 36 261 1287 21
Fragments arranged by size:
1287 261 36 21
BsrDI GCAATG_nn'
Cuts at: 0 245 542 1605
Size: 245 297 1063
Fragments arranged by size:
1063 297 245
BsrFI r'CCGG_y
Cuts at: 0 1453 1605
Size: 1453 152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 247 312 544 1605
Size: 247 65 232 1061
Fragments arranged by size:
1061 247 232 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 498 597 682 916 1605
Size: 498 99 85 234 689
Fragments arranged by size:
689 498 234 99 85
BstYI r'GATC_y
Cuts at: 0 38 781 1605
Size: 38 743 824
Fragments arranged by size:
824 743 38
Bsu36I CC'TnA_GG
Cuts at: 0 215 1605
Size: 215 1390
BtgI C'CryG_G
Cuts at: 0 590 596 714 864 1595 1605
Size: 590 6 118 150 731 10
Fragments arranged by size:
731 590 150 118 10 6
BtsI GCAGTG_nn'
Cuts at: 0 32 1364 1605
Size: 32 1332 241
Fragments arranged by size:
1332 241 32
ClaI AT'CG_AT
Cuts at: 0 170 1605
Size: 170 1435
DraIII CAC_nnn'GTG
Cuts at: 0 1389 1605
Size: 1389 216
EaeI y'GGCC_r
Cuts at: 0 593 1605
Size: 593 1012
EarI CTCTTCn'nnn_
Cuts at: 0 105 130 1605
Size: 105 25 1475
Fragments arranged by size:
1475 105 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 272 409 422 658 665 1073 1297
Size: 89 183 137 13 236 7 408 224
Cuts at: 1297 1455 1605
Size: 158 150
Fragments arranged by size:
408 236 224 183 158 150 137 89
13 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1470 1605
Size: 1470 135
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1605
Size: 210 1395
EcoRV GAT'ATC
Cuts at: 0 174 1605
Size: 174 1431
HaeII r_GCGC'y
Cuts at: 0 10 948 1605
Size: 10 938 657
Fragments arranged by size:
938 657 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 788 802 820 834 1124 1156
Size: 23 32 733 14 18 14 290 32
Cuts at: 1156 1190 1222 1242 1274 1400 1432 1605
Size: 34 32 20 32 126 32 173
Fragments arranged by size:
733 290 173 126 34 32 32 32
32 32 23 20 18 14 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1176 1377 1605
Size: 809 367 201 228
Fragments arranged by size:
809 367 228 201
MscI TGG'CCA
Cuts at: 0 595 1605
Size: 595 1010
MslI CAynn'nnrTG
Cuts at: 0 595 1426 1605
Size: 595 831 179
Fragments arranged by size:
831 595 179
MspA1I CmG'CkG
Cuts at: 0 912 1605
Size: 912 693
NcoI C'CATG_G
Cuts at: 0 590 596 714 864 1595 1605
Size: 590 6 118 150 731 10
Fragments arranged by size:
731 590 150 118 10 6
NdeI CA'TA_TG
Cuts at: 0 1326 1605
Size: 1326 279
NspI r_CATG'y
Cuts at: 0 442 1018 1605
Size: 442 576 587
Fragments arranged by size:
587 576 442
PflMI CCAn_nnn'nTGG
Cuts at: 0 992 1389 1605
Size: 992 397 216
Fragments arranged by size:
992 397 216
PfoI T'CCnGG_A
Cuts at: 0 674 1605
Size: 674 931
PpuMI rG'GwC_Cy
Cuts at: 0 210 1605
Size: 210 1395
PstI C_TGCA'G
Cuts at: 0 47 1605
Size: 47 1558
PvuII CAG'CTG
Cuts at: 0 912 1605
Size: 912 693
SanDI GG'GwC_CC
Cuts at: 0 210 1605
Size: 210 1395
SapI GCTCTTCn'nnn_
Cuts at: 0 130 1605
Size: 130 1475
SbfI CC_TGCA'GG
Cuts at: 0 47 1605
Size: 47 1558
SexAI A'CCwGG_T
Cuts at: 0 76 1605
Size: 76 1529
SfcI C'TryA_G
Cuts at: 0 43 444 1605
Size: 43 401 1161
Fragments arranged by size:
1161 401 43
SphI G_CATG'C
Cuts at: 0 442 1018 1605
Size: 442 576 587
Fragments arranged by size:
587 576 442
StuI AGG'CCT
Cuts at: 0 422 1605
Size: 422 1183
StyI C'CwwG_G
Cuts at: 0 56 224 590 596 714 864 1595 1605
Size: 56 168 366 6 118 150 731 10
Fragments arranged by size:
731 366 168 150 118 56 10 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 1605
Size: 231 1374
Enzymes that do cut:
AarI AcuI AfeI ApoI BamHI BanI BanII BbsI
BclI BglI Bme1580I BpmI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI
PflMI PfoI PpuMI PstI PvuII SanDI SapI SbfI
SexAI SfcI SphI StuI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI