Restriction map for
pFN21AE6397
(Linear) (Six-base) MAPSORT of: KIEE6397.seq Check: 1235 from: 1 to: 669
KIEE6397.orf
With 182 enzymes: SgfI *
March 16, 2015 11:31 ..
AccI GT'mk_AC
Cuts at: 0 533 669
Size: 533 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 232 266 669
Size: 219 13 34 403
Fragments arranged by size:
403 219 34 13
AleI CACnn'nnGTG
Cuts at: 0 401 580 669
Size: 401 179 89
Fragments arranged by size:
401 179 89
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 669
Size: 76 593
AlwNI CAG_nnn'CTG
Cuts at: 0 414 544 631 669
Size: 414 130 87 38
Fragments arranged by size:
414 130 87 38
ApaI G_GGCC'C
Cuts at: 0 42 669
Size: 42 627
AvaI C'yCGr_G
Cuts at: 0 439 669
Size: 439 230
BanI G'GyrC_C
Cuts at: 0 94 324 550 669
Size: 94 230 226 119
Fragments arranged by size:
230 226 119 94
BanII G_rGCy'C
Cuts at: 0 42 669
Size: 42 627
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 669
Size: 272 397
Bme1580I G_kGCm'C
Cuts at: 0 42 65 446 553 622 669
Size: 42 23 381 107 69 47
Fragments arranged by size:
381 107 69 47 42 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 636 669
Size: 510 126 33
Fragments arranged by size:
510 126 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 444 474 505 535 669
Size: 444 30 31 30 134
Fragments arranged by size:
444 134 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 261 306 513 669
Size: 261 45 207 156
Fragments arranged by size:
261 207 156 45
BseYI C'CCAG_C
Cuts at: 0 3 90 537 669
Size: 3 87 447 132
Fragments arranged by size:
447 132 87 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 514 669
Size: 417 97 155
Fragments arranged by size:
417 155 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 276 669
Size: 276 393
Bsp1286I G_dGCh'C
Cuts at: 0 42 65 446 553 622 669
Size: 42 23 381 107 69 47
Fragments arranged by size:
381 107 69 47 42 23
BssSI C'ACGA_G
Cuts at: 0 99 669
Size: 99 570
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 669
Size: 156 513
BtgI C'CryG_G
Cuts at: 0 79 669
Size: 79 590
DraIII CAC_nnn'GTG
Cuts at: 0 381 669
Size: 381 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 232 266 510 636 669
Size: 219 13 34 244 126 33
Fragments arranged by size:
244 219 126 34 33 13
EcoO109I rG'GnC_Cy
Cuts at: 0 39 611 657 669
Size: 39 572 46 12
Fragments arranged by size:
572 46 39 12
EcoRV GAT'ATC
Cuts at: 0 254 669
Size: 254 415
HaeII r_GCGC'y
Cuts at: 0 211 669
Size: 211 458
MslI CAynn'nnrTG
Cuts at: 0 58 401 580 669
Size: 58 343 179 89
Fragments arranged by size:
343 179 89 58
NspI r_CATG'y
Cuts at: 0 450 669
Size: 450 219
PasI CC'CwG_GG
Cuts at: 0 558 669
Size: 558 111
PpuMI rG'GwC_Cy
Cuts at: 0 657 669
Size: 657 12
PspOMI G'GGCC_C
Cuts at: 0 38 669
Size: 38 631
PstI C_TGCA'G
Cuts at: 0 636 669
Size: 636 33
SanDI GG'GwC_CC
Cuts at: 0 657 669
Size: 657 12
SfcI C'TryA_G
Cuts at: 0 632 669
Size: 632 37
SmaI CCC'GGG
Cuts at: 0 441 669
Size: 441 228
StyI C'CwwG_G
Cuts at: 0 614 641 669
Size: 614 27 28
Fragments arranged by size:
614 28 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 669
Size: 82 587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 444 476 669
Size: 367 32 45 32 193
Fragments arranged by size:
367 193 45 32 32
XmaI C'CCGG_G
Cuts at: 0 439 669
Size: 439 230
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbsI Bme1580I BpmI BsaXI BseRI BseYI BsgI
BsmBI Bsp1286I BssSI BstXI BtgI DraIII Eco57MI EcoO109I
EcoRV HaeII MslI NspI PasI PpuMI PspOMI PstI
SanDI SfcI SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI