(Linear) (Six-base) MAPSORT of: KIEE6396.seq  Check: 2159  from: 1  to: 1314



KIEE6396.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:31  ..

AccI GT'mk_AC

Cuts at:      0     393    1314 
   Size:        393     921
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     341    1314 
   Size:        140     201     973

  Fragments arranged by size: 

                 973     201     140
 
ApoI r'AATT_y

Cuts at:      0     950    1302    1314 
   Size:        950     352      12

  Fragments arranged by size: 

                 950     352      12
 
BanI G'GyrC_C

Cuts at:      0     402    1314 
   Size:        402     912
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      97    1314 
   Size:         97    1217
 
BclI T'GATC_A

Cuts at:      0     740    1238    1314 
   Size:        740     498      76

  Fragments arranged by size: 

                 740     498      76
 
BfrBI ATG'CAT

Cuts at:      0     411    1314 
   Size:        411     903
 
BglII A'GATC_T

Cuts at:      0     233    1314 
   Size:        233    1081
 
Bme1580I G_kGCm'C

Cuts at:      0     646    1314 
   Size:        646     668
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382    1314 
   Size:        382     932
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     510    1024    1314 
   Size:        510     514     290

  Fragments arranged by size: 

                 514     510     290
 
BsaAI yAC'GTr

Cuts at:      0     641    1314 
   Size:        641     673
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    1314 
   Size:        621     693
 
BsiHKAI G_wGCw'C

Cuts at:      0     377    1314 
   Size:        377     937
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     646    1314 
   Size:        377     269     668

  Fragments arranged by size: 

                 668     377     269
 
BspHI T'CATG_A

Cuts at:      0     743    1314 
   Size:        743     571
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     643    1314 
   Size:        643     671
 
BsrDI GCAATG_nn'

Cuts at:      0     598    1314 
   Size:        598     716
 
BstEII G'GTnAC_C

Cuts at:      0     270    1314 
   Size:        270    1044
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267    1314 
   Size:        267    1047
 
BstYI r'GATC_y

Cuts at:      0     195     233    1314 
   Size:        195      38    1081

  Fragments arranged by size: 

                1081     195      38
 
Bsu36I CC'TnA_GG

Cuts at:      0     605    1314 
   Size:        605     709
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      50    1314 
   Size:         50    1264
 
EaeI y'GGCC_r

Cuts at:      0     224     701    1314 
   Size:        224     477     613

  Fragments arranged by size: 

                 613     477     224
 
EarI CTCTTCn'nnn_

Cuts at:      0     508    1088    1314 
   Size:        508     580     226

  Fragments arranged by size: 

                 580     508     226
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     341     382    1314 
   Size:        140     201      41     932

  Fragments arranged by size: 

                 932     201     140      41
 
HincII GTy'rAC

Cuts at:      0     827    1314 
   Size:        827     487
 
HindIII A'AGCT_T

Cuts at:      0     614     897    1314 
   Size:        614     283     417

  Fragments arranged by size: 

                 614     417     283
 
MfeI C'AATT_G

Cuts at:      0     857    1314 
   Size:        857     457
 
MscI TGG'CCA

Cuts at:      0     226     703    1314 
   Size:        226     477     611

  Fragments arranged by size: 

                 611     477     226
 
MslI CAynn'nnrTG

Cuts at:      0     430     709     844    1314 
   Size:        430     279     135     470

  Fragments arranged by size: 

                 470     430     279     135
 
MspA1I CmG'CkG

Cuts at:      0     495    1155    1314 
   Size:        495     660     159

  Fragments arranged by size: 

                 660     495     159
 
NsiI A_TGCA'T

Cuts at:      0     413    1314 
   Size:        413     901
 
PmlI CAC'GTG

Cuts at:      0     641    1314 
   Size:        641     673
 
PvuII CAG'CTG

Cuts at:      0     495    1155    1314 
   Size:        495     660     159

  Fragments arranged by size: 

                 660     495     159
 
SfcI C'TryA_G

Cuts at:      0      44    1314 
   Size:         44    1270
 
StyI C'CwwG_G

Cuts at:      0    1172    1314 
   Size:       1172     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289    1314 
   Size:        289    1025
 
XbaI T'CTAG_A

Cuts at:      0     681     999    1314 
   Size:        681     318     315

  Fragments arranged by size: 

                 681     318     315
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BanI     BbsI     BclI    BfrBI    BglII
 Bme1580I     BpmI     BsaI    BsaAI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     EaeI
     EarI  Eco57MI   HincII  HindIII     MfeI     MscI     MslI   MspA1I
     NsiI     PmlI    PvuII     SfcI     StyI    TaqII     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI