Restriction map for
pFN21AE6394
(Linear) (Six-base) MAPSORT of: KIEE6394.seq Check: 8326 from: 1 to: 1029
KIEE6394.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 551 1029
Size: 551 478
AccI GT'mk_AC
Cuts at: 0 446 1029
Size: 446 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 817 823 1029
Size: 526 291 6 206
Fragments arranged by size:
526 291 206 6
AleI CACnn'nnGTG
Cuts at: 0 859 1029
Size: 859 170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 1029
Size: 877 152
ApaI G_GGCC'C
Cuts at: 0 645 1029
Size: 645 384
AvrII C'CTAG_G
Cuts at: 0 21 786 1029
Size: 21 765 243
Fragments arranged by size:
765 243 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 764 797 1029
Size: 764 33 232
Fragments arranged by size:
764 232 33
BanII G_rGCy'C
Cuts at: 0 10 645 980 1029
Size: 10 635 335 49
Fragments arranged by size:
635 335 49 10
BbvCI CC'TCA_GC
Cuts at: 0 227 1029
Size: 227 802
BciVI GTATCCnnnnn_n'
Cuts at: 0 765 1029
Size: 765 264
Bme1580I G_kGCm'C
Cuts at: 0 617 645 1029
Size: 617 28 384
Fragments arranged by size:
617 384 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1029
Size: 1011 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 994 1029
Size: 850 144 35
Fragments arranged by size:
850 144 35
Bpu10I CC'TnA_GC
Cuts at: 0 227 403 1029
Size: 227 176 626
Fragments arranged by size:
626 227 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1029
Size: 577 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 654 1029
Size: 654 375
BsaBI GATnn'nnATC
Cuts at: 0 692 1029
Size: 692 337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 443 691 987 1000 1029
Size: 253 190 248 296 13 29
Fragments arranged by size:
296 253 248 190 29 13
BseYI C'CCAG_C
Cuts at: 0 78 535 1029
Size: 78 457 494
Fragments arranged by size:
494 457 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1029
Size: 334 695
BsiEI CG_ry'CG
Cuts at: 0 98 1029
Size: 98 931
BsiHKAI G_wGCw'C
Cuts at: 0 866 899 980 1029
Size: 866 33 81 49
Fragments arranged by size:
866 81 49 33
Bsp1286I G_dGCh'C
Cuts at: 0 10 617 645 866 899 980 1029
Size: 10 607 28 221 33 81 49
Fragments arranged by size:
607 221 81 49 33 28 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 1029
Size: 551 478
BsrBI CCG'CTC
Cuts at: 0 263 1029
Size: 263 766
BsrFI r'CCGG_y
Cuts at: 0 287 1029
Size: 287 742
BstAPI GCAn_nnn'nTGC
Cuts at: 0 67 1029
Size: 67 962
BstXI CCAn_nnnn'nTGG
Cuts at: 0 344 573 671 1029
Size: 344 229 98 358
Fragments arranged by size:
358 344 229 98
BstYI r'GATC_y
Cuts at: 0 755 1029
Size: 755 274
BtsI GCAGTG_nn'
Cuts at: 0 61 187 1029
Size: 61 126 842
Fragments arranged by size:
842 126 61
EaeI y'GGCC_r
Cuts at: 0 254 734 821 1029
Size: 254 480 87 208
Fragments arranged by size:
480 254 208 87
EarI CTCTTCn'nnn_
Cuts at: 0 211 1029
Size: 211 818
EcoICRI GAG'CTC
Cuts at: 0 978 1029
Size: 978 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 817 823 850 994 1029
Size: 526 291 6 27 144 35
Fragments arranged by size:
526 291 144 35 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 505 944 1029
Size: 505 439 85
Fragments arranged by size:
505 439 85
EcoO109I rG'GnC_Cy
Cuts at: 0 159 435 498 519 641 950 990 1029
Size: 159 276 63 21 122 309 40 39
Fragments arranged by size:
309 276 159 122 63 40 39 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1029
Size: 982 32 15
Fragments arranged by size:
982 32 15
MscI TGG'CCA
Cuts at: 0 736 823 1029
Size: 736 87 206
Fragments arranged by size:
736 206 87
MslI CAynn'nnrTG
Cuts at: 0 859 1029
Size: 859 170
MspA1I CmG'CkG
Cuts at: 0 778 1029
Size: 778 251
NaeI GCC'GGC
Cuts at: 0 289 1029
Size: 289 740
NgoMIV G'CCGG_C
Cuts at: 0 287 1029
Size: 287 742
NspI r_CATG'y
Cuts at: 0 298 908 1029
Size: 298 610 121
Fragments arranged by size:
610 298 121
PasI CC'CwG_GG
Cuts at: 0 10 438 523 1029
Size: 10 428 85 506
Fragments arranged by size:
506 428 85 10
PfoI T'CCnGG_A
Cuts at: 0 305 866 884 1029
Size: 305 561 18 145
Fragments arranged by size:
561 305 145 18
PpuMI rG'GwC_Cy
Cuts at: 0 519 950 990 1029
Size: 519 431 40 39
Fragments arranged by size:
519 431 40 39
PspOMI G'GGCC_C
Cuts at: 0 641 1029
Size: 641 388
PstI C_TGCA'G
Cuts at: 0 44 710 1029
Size: 44 666 319
Fragments arranged by size:
666 319 44
SacI G_AGCT'C
Cuts at: 0 980 1029
Size: 980 49
SanDI GG'GwC_CC
Cuts at: 0 519 950 1029
Size: 519 431 79
Fragments arranged by size:
519 431 79
SfcI C'TryA_G
Cuts at: 0 40 706 1029
Size: 40 666 323
Fragments arranged by size:
666 323 40
SmlI C'TyrA_G
Cuts at: 0 592 1029
Size: 592 437
SphI G_CATG'C
Cuts at: 0 298 1029
Size: 298 731
StuI AGG'CCT
Cuts at: 0 511 1029
Size: 511 518
StyI C'CwwG_G
Cuts at: 0 21 122 786 1029
Size: 21 101 664 243
Fragments arranged by size:
664 243 101 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 677 751 1029
Size: 430 247 74 278
Fragments arranged by size:
430 278 247 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 478 1029
Size: 478 551
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApaI AvrII BaeI
BanII BbvCI BciVI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstXI BstYI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI MslI
MspA1I NaeI NgoMIV NspI PasI PfoI PpuMI PspOMI
PstI SacI SanDI SfcI SmlI SphI StuI StyI
TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BbeI BbsI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BsaAI BsaHI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI