Restriction map for
pFN21AE6392
(Linear) (Six-base) MAPSORT of: KIEE6392.seq Check: 78 from: 1 to: 1254
KIEE6392.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 1202 1254
Size: 1202 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 595 1254
Size: 64 531 659
Fragments arranged by size:
659 531 64
AflII C'TTAA_G
Cuts at: 0 220 1254
Size: 220 1034
AhdI GACnn_n'nnGTC
Cuts at: 0 104 1114 1254
Size: 104 1010 140
Fragments arranged by size:
1010 140 104
AleI CACnn'nnGTG
Cuts at: 0 1210 1254
Size: 1210 44
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 696 1254
Size: 696 558
ApoI r'AATT_y
Cuts at: 0 167 246 435 906 1076 1254
Size: 167 79 189 471 170 178
Fragments arranged by size:
471 189 178 170 167 79
BanI G'GyrC_C
Cuts at: 0 25 1254
Size: 25 1229
BanII G_rGCy'C
Cuts at: 0 490 1254
Size: 490 764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 587 1217 1254
Size: 305 282 630 37
Fragments arranged by size:
630 305 282 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 837 1254
Size: 112 725 417
Fragments arranged by size:
725 417 112
BsaAI yAC'GTr
Cuts at: 0 1139 1254
Size: 1139 115
BseYI C'CCAG_C
Cuts at: 0 1163 1254
Size: 1163 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1254
Size: 847 407
BsiEI CG_ry'CG
Cuts at: 0 242 1254
Size: 242 1012
BsiHKAI G_wGCw'C
Cuts at: 0 490 939 1254
Size: 490 449 315
Fragments arranged by size:
490 449 315
BsmBI CGTCTCn'nnnn_
Cuts at: 0 229 1254
Size: 229 1025
Bsp1286I G_dGCh'C
Cuts at: 0 490 939 1254
Size: 490 449 315
Fragments arranged by size:
490 449 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 819 1254
Size: 819 435
BsrBI CCG'CTC
Cuts at: 0 794 1254
Size: 794 460
BsrDI GCAATG_nn'
Cuts at: 0 488 572 815 1038 1254
Size: 488 84 243 223 216
Fragments arranged by size:
488 243 223 216 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 634 710 1254
Size: 634 76 544
Fragments arranged by size:
634 544 76
BstYI r'GATC_y
Cuts at: 0 174 1125 1254
Size: 174 951 129
Fragments arranged by size:
951 174 129
BtgI C'CryG_G
Cuts at: 0 49 1254
Size: 49 1205
BtsI GCAGTG_nn'
Cuts at: 0 101 668 678 1254
Size: 101 567 10 576
Fragments arranged by size:
576 567 101 10
DraI TTT'AAA
Cuts at: 0 165 1254
Size: 165 1089
EarI CTCTTCn'nnn_
Cuts at: 0 840 1254
Size: 840 414
EcoICRI GAG'CTC
Cuts at: 0 488 1254
Size: 488 766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 305 587 595 1217 1254
Size: 64 241 282 8 622 37
Fragments arranged by size:
622 282 241 64 37 8
EcoO109I rG'GnC_Cy
Cuts at: 0 1108 1254
Size: 1108 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 625 1254
Size: 625 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 90 122 761 793 974 1006 1100 1132
Size: 90 32 639 32 181 32 94 32
Cuts at: 1132 1254
Size: 122
Fragments arranged by size:
639 181 122 94 90 32 32 32
32
HincII GTy'rAC
Cuts at: 0 506 552 1254
Size: 506 46 702
Fragments arranged by size:
702 506 46
HindIII A'AGCT_T
Cuts at: 0 602 1254
Size: 602 652
HpaI GTT'AAC
Cuts at: 0 506 1254
Size: 506 748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1254
Size: 924 330
MslI CAynn'nnrTG
Cuts at: 0 355 632 708 1210 1254
Size: 355 277 76 502 44
Fragments arranged by size:
502 355 277 76 44
NcoI C'CATG_G
Cuts at: 0 49 1254
Size: 49 1205
PpuMI rG'GwC_Cy
Cuts at: 0 1108 1254
Size: 1108 146
PstI C_TGCA'G
Cuts at: 0 675 801 1093 1254
Size: 675 126 292 161
Fragments arranged by size:
675 292 161 126
SacI G_AGCT'C
Cuts at: 0 490 1254
Size: 490 764
SbfI CC_TGCA'GG
Cuts at: 0 1093 1254
Size: 1093 161
ScaI AGT'ACT
Cuts at: 0 148 1254
Size: 148 1106
SfcI C'TryA_G
Cuts at: 0 671 797 1089 1254
Size: 671 126 292 165
Fragments arranged by size:
671 292 165 126
SmlI C'TyrA_G
Cuts at: 0 220 1254
Size: 220 1034
StyI C'CwwG_G
Cuts at: 0 49 581 595 1254
Size: 49 532 14 659
Fragments arranged by size:
659 532 49 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 256 594 1254
Size: 256 338 660
Fragments arranged by size:
660 338 256
TatI w'GTAC_w
Cuts at: 0 146 1254
Size: 146 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 668 700 1254
Size: 668 32 554
Fragments arranged by size:
668 554 32
Tth111I GACn'n_nGTC
Cuts at: 0 1003 1254
Size: 1003 251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 520 1254
Size: 108 412 734
Fragments arranged by size:
734 412 108
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI ApoI BanI
BanII BpmI BsaI BsaAI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI BtgI
BtsI DraI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I
HincII HindIII HpaI MmeI MslI NcoI PpuMI PstI
SacI SbfI ScaI SfcI SmlI StyI TaqII TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI
BsiWI BsmI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI
ZraI