Restriction map for
pFN21AE6391
(Linear) (Six-base) MAPSORT of: KIEE6391.seq Check: 9992 from: 1 to: 1242
KIEE6391.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 341 1242
Size: 341 901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1242
Size: 908 334
AflIII A'CryG_T
Cuts at: 0 281 1153 1242
Size: 281 872 89
Fragments arranged by size:
872 281 89
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1242
Size: 414 828
ApaLI G'TGCA_C
Cuts at: 0 277 1242
Size: 277 965
ApoI r'AATT_y
Cuts at: 0 290 321 973 1012 1242
Size: 290 31 652 39 230
Fragments arranged by size:
652 290 230 39 31
AvaI C'yCGr_G
Cuts at: 0 1208 1242
Size: 1208 34
BanII G_rGCy'C
Cuts at: 0 360 1242
Size: 360 882
BbsI GAAGACnn'nnnn_
Cuts at: 0 1134 1242
Size: 1134 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1161 1195 1242
Size: 1161 34 47
Fragments arranged by size:
1161 47 34
BclI T'GATC_A
Cuts at: 0 95 1242
Size: 95 1147
Bme1580I G_kGCm'C
Cuts at: 0 281 1242
Size: 281 961
BmgBI CAC'GTC
Cuts at: 0 243 1242
Size: 243 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 601 1004 1242
Size: 436 165 403 238
Fragments arranged by size:
436 403 238 165
BsaAI yAC'GTr
Cuts at: 0 793 1154 1242
Size: 793 361 88
Fragments arranged by size:
793 361 88
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1242
Size: 91 1151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 88 815 1242
Size: 79 9 727 427
Fragments arranged by size:
727 427 79 9
BsiHKAI G_wGCw'C
Cuts at: 0 74 281 1209 1242
Size: 74 207 928 33
Fragments arranged by size:
928 207 74 33
BsmI GAATG_Cn'
Cuts at: 0 198 839 1242
Size: 198 641 403
Fragments arranged by size:
641 403 198
Bsp1286I G_dGCh'C
Cuts at: 0 74 281 360 1209 1242
Size: 74 207 79 849 33
Fragments arranged by size:
849 207 79 74 33
BsrFI r'CCGG_y
Cuts at: 0 137 404 1242
Size: 137 267 838
Fragments arranged by size:
838 267 137
BsrGI T'GTAC_A
Cuts at: 0 862 1242
Size: 862 380
BssSI C'ACGA_G
Cuts at: 0 73 1180 1242
Size: 73 1107 62
Fragments arranged by size:
1107 73 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1116 1242
Size: 1116 126
BtgI C'CryG_G
Cuts at: 0 108 1242
Size: 108 1134
BtsI GCAGTG_nn'
Cuts at: 0 148 236 1209 1242
Size: 148 88 973 33
Fragments arranged by size:
973 148 88 33
EarI CTCTTCn'nnn_
Cuts at: 0 254 581 662 1242
Size: 254 327 81 580
Fragments arranged by size:
580 327 254 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 1242
Size: 172 1070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 601 908 1004 1242
Size: 436 165 307 96 238
Fragments arranged by size:
436 307 238 165 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 1242
Size: 427 815
EcoO109I rG'GnC_Cy
Cuts at: 0 420 919 1242
Size: 420 499 323
Fragments arranged by size:
499 420 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1068 1242
Size: 1068 174
HaeII r_GCGC'y
Cuts at: 0 414 1242
Size: 414 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 1242
Size: 604 32 606
Fragments arranged by size:
606 604 32
HindIII A'AGCT_T
Cuts at: 0 18 1242
Size: 18 1224
MfeI C'AATT_G
Cuts at: 0 221 1242
Size: 221 1021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 1242
Size: 233 1009
MslI CAynn'nnrTG
Cuts at: 0 832 1242
Size: 832 410
MspA1I CmG'CkG
Cuts at: 0 110 158 571 1242
Size: 110 48 413 671
Fragments arranged by size:
671 413 110 48
NaeI GCC'GGC
Cuts at: 0 139 406 1242
Size: 139 267 836
Fragments arranged by size:
836 267 139
NgoMIV G'CCGG_C
Cuts at: 0 137 404 1242
Size: 137 267 838
Fragments arranged by size:
838 267 137
NspI r_CATG'y
Cuts at: 0 604 732 1242
Size: 604 128 510
Fragments arranged by size:
604 510 128
PasI CC'CwG_GG
Cuts at: 0 50 1242
Size: 50 1192
PfoI T'CCnGG_A
Cuts at: 0 149 1242
Size: 149 1093
PmlI CAC'GTG
Cuts at: 0 793 1154 1242
Size: 793 361 88
Fragments arranged by size:
793 361 88
PpuMI rG'GwC_Cy
Cuts at: 0 919 1242
Size: 919 323
PshAI GACnn'nnGTC
Cuts at: 0 719 1242
Size: 719 523
PsiI TTA'TAA
Cuts at: 0 811 1242
Size: 811 431
PstI C_TGCA'G
Cuts at: 0 163 353 365 1242
Size: 163 190 12 877
Fragments arranged by size:
877 190 163 12
PvuII CAG'CTG
Cuts at: 0 158 1242
Size: 158 1084
SacII CC_GC'GG
Cuts at: 0 111 1242
Size: 111 1131
SbfI CC_TGCA'GG
Cuts at: 0 365 1242
Size: 365 877
SfcI C'TryA_G
Cuts at: 0 159 349 361 1242
Size: 159 190 12 881
Fragments arranged by size:
881 190 159 12
SphI G_CATG'C
Cuts at: 0 732 1242
Size: 732 510
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 1242
Size: 18 1224
TatI w'GTAC_w
Cuts at: 0 862 909 1242
Size: 862 47 333
Fragments arranged by size:
862 333 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1194 1242
Size: 1194 48
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI AvaI BanII
BbsI BcgI BclI Bme1580I BmgBI BpmI BsaAI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrFI BsrGI BssSI BstAPI
BtgI BtsI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII MfeI MmeI MslI MspA1I NaeI
NgoMIV NspI PasI PfoI PmlI PpuMI PshAI PsiI
PstI PvuII SacII SbfI SfcI SphI TaqII TatI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BssHII BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PpiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI