Restriction map for
pFN21AE6389
(Linear) (Six-base) MAPSORT of: KIEE6389.seq Check: 1944 from: 1 to: 1155
KIEE6389.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 653 1146 1155
Size: 653 493 9
Fragments arranged by size:
653 493 9
Acc65I G'GTAC_C
Cuts at: 0 1129 1155
Size: 1129 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 365 1155
Size: 58 307 790
Fragments arranged by size:
790 307 58
AlwNI CAG_nnn'CTG
Cuts at: 0 343 1155
Size: 343 812
ApaI G_GGCC'C
Cuts at: 0 475 1155
Size: 475 680
AvaI C'yCGr_G
Cuts at: 0 410 1155
Size: 410 745
BanI G'GyrC_C
Cuts at: 0 123 1054 1129 1155
Size: 123 931 75 26
Fragments arranged by size:
931 123 75 26
BanII G_rGCy'C
Cuts at: 0 475 732 962 1155
Size: 475 257 230 193
Fragments arranged by size:
475 257 230 193
BglI GCCn_nnn'nGGC
Cuts at: 0 796 1155
Size: 796 359
Bme1580I G_kGCm'C
Cuts at: 0 137 475 1155
Size: 137 338 680
Fragments arranged by size:
680 338 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 802 976 1155
Size: 320 482 174 179
Fragments arranged by size:
482 320 179 174
BsaWI w'CCGG_w
Cuts at: 0 5 1155
Size: 5 1150
BseYI C'CCAG_C
Cuts at: 0 26 1011 1155
Size: 26 985 144
Fragments arranged by size:
985 144 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 755 1155
Size: 166 589 400
Fragments arranged by size:
589 400 166
BsiHKAI G_wGCw'C
Cuts at: 0 732 816 997 1155
Size: 732 84 181 158
Fragments arranged by size:
732 181 158 84
BsmI GAATG_Cn'
Cuts at: 0 644 1155
Size: 644 511
Bsp1286I G_dGCh'C
Cuts at: 0 137 475 732 816 962 997 1155
Size: 137 338 257 84 146 35 158
Fragments arranged by size:
338 257 158 146 137 84 35
BspEI T'CCGG_A
Cuts at: 0 5 1155
Size: 5 1150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 619 1155
Size: 619 536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 66 1155
Size: 66 1089
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1086 1155
Size: 1086 69
BstYI r'GATC_y
Cuts at: 0 607 1155
Size: 607 548
BtgI C'CryG_G
Cuts at: 0 266 683 1155
Size: 266 417 472
Fragments arranged by size:
472 417 266
BtsI GCAGTG_nn'
Cuts at: 0 314 964 1155
Size: 314 650 191
Fragments arranged by size:
650 314 191
EaeI y'GGCC_r
Cuts at: 0 189 644 947 1076 1155
Size: 189 455 303 129 79
Fragments arranged by size:
455 303 189 129 79
EarI CTCTTCn'nnn_
Cuts at: 0 180 395 1155
Size: 180 215 760
Fragments arranged by size:
760 215 180
EcoICRI GAG'CTC
Cuts at: 0 730 1155
Size: 730 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 320 365 802 976 1155
Size: 58 262 45 437 174 179
Fragments arranged by size:
437 262 179 174 58 45
EcoO109I rG'GnC_Cy
Cuts at: 0 472 563 702 1155
Size: 472 91 139 453
Fragments arranged by size:
472 453 139 91
EcoRV GAT'ATC
Cuts at: 0 496 1155
Size: 496 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 485 512 517 555 587 1155
Size: 480 5 27 5 38 32 568
Fragments arranged by size:
568 480 38 32 27 5 5
HincII GTy'rAC
Cuts at: 0 171 459 1155
Size: 171 288 696
Fragments arranged by size:
696 288 171
KpnI G_GTAC'C
Cuts at: 0 1133 1155
Size: 1133 22
MfeI C'AATT_G
Cuts at: 0 128 1155
Size: 128 1027
MscI TGG'CCA
Cuts at: 0 646 949 1078 1155
Size: 646 303 129 77
Fragments arranged by size:
646 303 129 77
MslI CAynn'nnrTG
Cuts at: 0 544 907 943 976 1155
Size: 544 363 36 33 179
Fragments arranged by size:
544 363 179 36 33
MspA1I CmG'CkG
Cuts at: 0 30 63 1011 1155
Size: 30 33 948 144
Fragments arranged by size:
948 144 33 30
NcoI C'CATG_G
Cuts at: 0 683 1155
Size: 683 472
NspI r_CATG'y
Cuts at: 0 108 214 1155
Size: 108 106 941
Fragments arranged by size:
941 108 106
PfoI T'CCnGG_A
Cuts at: 0 95 953 1155
Size: 95 858 202
Fragments arranged by size:
858 202 95
PpuMI rG'GwC_Cy
Cuts at: 0 563 1155
Size: 563 592
PspOMI G'GGCC_C
Cuts at: 0 471 1155
Size: 471 684
PstI C_TGCA'G
Cuts at: 0 918 1155
Size: 918 237
PvuII CAG'CTG
Cuts at: 0 63 1011 1155
Size: 63 948 144
Fragments arranged by size:
948 144 63
SacI G_AGCT'C
Cuts at: 0 732 1155
Size: 732 423
SfcI C'TryA_G
Cuts at: 0 832 914 1155
Size: 832 82 241
Fragments arranged by size:
832 241 82
SmaI CCC'GGG
Cuts at: 0 412 1155
Size: 412 743
StyI C'CwwG_G
Cuts at: 0 683 710 863 1155
Size: 683 27 153 292
Fragments arranged by size:
683 292 153 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 942 1008 1155
Size: 942 66 147
Fragments arranged by size:
942 147 66
XmaI C'CCGG_G
Cuts at: 0 410 1155
Size: 410 745
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI AvaI BanI BanII
BglI Bme1580I BpmI BsaWI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BstAPI BstXI BstYI BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I HincII
KpnI MfeI MscI MslI MspA1I NcoI NspI PfoI
PpuMI PspOMI PstI PvuII SacI SfcI SmaI StyI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI