Restriction map for
pFN21AE6383
(Linear) (Six-base) MAPSORT of: KIEE6383.seq Check: 2370 from: 1 to: 987
KIEE6383.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 987
Size: 435 552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 987
Size: 726 261
ApoI r'AATT_y
Cuts at: 0 832 987
Size: 832 155
BanI G'GyrC_C
Cuts at: 0 682 987
Size: 682 305
BanII G_rGCy'C
Cuts at: 0 86 867 987
Size: 86 781 120
Fragments arranged by size:
781 120 86
BciVI GTATCCnnnnn_n'
Cuts at: 0 798 987
Size: 798 189
BclI T'GATC_A
Cuts at: 0 575 840 987
Size: 575 265 147
Fragments arranged by size:
575 265 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 987
Size: 588 399
BsaAI yAC'GTr
Cuts at: 0 633 987
Size: 633 354
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 103 987
Size: 94 9 884
Fragments arranged by size:
884 94 9
BseYI C'CCAG_C
Cuts at: 0 14 29 987
Size: 14 15 958
Fragments arranged by size:
958 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 987
Size: 696 291
BsiEI CG_ry'CG
Cuts at: 0 69 987
Size: 69 918
BsiHKAI G_wGCw'C
Cuts at: 0 86 867 987
Size: 86 781 120
Fragments arranged by size:
781 120 86
BsmI GAATG_Cn'
Cuts at: 0 783 987
Size: 783 204
Bsp1286I G_dGCh'C
Cuts at: 0 86 867 987
Size: 86 781 120
Fragments arranged by size:
781 120 86
BspHI T'CATG_A
Cuts at: 0 689 897 987
Size: 689 208 90
Fragments arranged by size:
689 208 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 987
Size: 113 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 682 778 987
Size: 682 96 209
Fragments arranged by size:
682 209 96
BstYI r'GATC_y
Cuts at: 0 719 987
Size: 719 268
BtgI C'CryG_G
Cuts at: 0 800 987
Size: 800 187
BtsI GCAGTG_nn'
Cuts at: 0 86 987
Size: 86 901
DraIII CAC_nnn'GTG
Cuts at: 0 673 987
Size: 673 314
EaeI y'GGCC_r
Cuts at: 0 39 66 492 987
Size: 39 27 426 495
Fragments arranged by size:
495 426 39 27
EagI C'GGCC_G
Cuts at: 0 66 987
Size: 66 921
EcoICRI GAG'CTC
Cuts at: 0 84 865 987
Size: 84 781 122
Fragments arranged by size:
781 122 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 987
Size: 435 552
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 751 987
Size: 751 236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 987
Size: 398 589
MscI TGG'CCA
Cuts at: 0 494 987
Size: 494 493
MspA1I CmG'CkG
Cuts at: 0 74 346 498 567 706 987
Size: 74 272 152 69 139 281
Fragments arranged by size:
281 272 152 139 74 69
NcoI C'CATG_G
Cuts at: 0 800 987
Size: 800 187
NotI GC'GGCC_GC
Cuts at: 0 66 987
Size: 66 921
PasI CC'CwG_GG
Cuts at: 0 199 987
Size: 199 788
PflMI CCAn_nnn'nTGG
Cuts at: 0 670 987
Size: 670 317
PstI C_TGCA'G
Cuts at: 0 566 987
Size: 566 421
PvuII CAG'CTG
Cuts at: 0 74 346 706 987
Size: 74 272 360 281
Fragments arranged by size:
360 281 272 74
SacI G_AGCT'C
Cuts at: 0 86 867 987
Size: 86 781 120
Fragments arranged by size:
781 120 86
SfcI C'TryA_G
Cuts at: 0 562 987
Size: 562 425
SmlI C'TyrA_G
Cuts at: 0 603 987
Size: 603 384
StyI C'CwwG_G
Cuts at: 0 800 987
Size: 800 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 987
Size: 183 804
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 195 227 579 611 987
Size: 195 32 352 32 376
Fragments arranged by size:
376 352 195 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 987
Size: 798 189
Enzymes that do cut:
AcuI AlfI ApoI BanI BanII BciVI BclI BpuEI
BsaAI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BstAPI BstYI BtgI BtsI DraIII EaeI
EagI EcoICRI Eco57MI FalI MmeI MscI MspA1I NcoI
NotI PasI PflMI PstI PvuII SacI SfcI SmlI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NdeI NgoMIV NheI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI