(Linear) (Six-base) MAPSORT of: KIEE6381.seq  Check: 3209  from: 1  to: 957



KIEE6381.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     887     957 
   Size:         25     862      70

  Fragments arranged by size: 

                 862      70      25
 
AhdI GACnn_n'nnGTC

Cuts at:      0     542     957 
   Size:        542     415
 
AlwNI CAG_nnn'CTG

Cuts at:      0     314     957 
   Size:        314     643
 
ApoI r'AATT_y

Cuts at:      0     496     753     957 
   Size:        496     257     204

  Fragments arranged by size: 

                 496     257     204
 
BamHI G'GATC_C

Cuts at:      0     187     957 
   Size:        187     770
 
BanI G'GyrC_C

Cuts at:      0     160     483     957 
   Size:        160     323     474

  Fragments arranged by size: 

                 474     323     160
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     538     957 
   Size:        538     419
 
BclI T'GATC_A

Cuts at:      0     305     570     957 
   Size:        305     265     387

  Fragments arranged by size: 

                 387     305     265
 
BglII A'GATC_T

Cuts at:      0     507     957 
   Size:        507     450
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     957 
   Size:        553     404
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     886     957 
   Size:        886      71
 
BseYI C'CCAG_C

Cuts at:      0     314     796     957 
   Size:        314     482     161

  Fragments arranged by size: 

                 482     314     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     957 
   Size:        236     721
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     529     957 
   Size:        529     428
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     208     644     957 
   Size:        208     436     313

  Fragments arranged by size: 

                 436     313     208
 
BstEII G'GTnAC_C

Cuts at:      0     709     957 
   Size:        709     248
 
BstYI r'GATC_y

Cuts at:      0     187     507     957 
   Size:        187     320     450

  Fragments arranged by size: 

                 450     320     187
 
EaeI y'GGCC_r

Cuts at:      0     929     957 
   Size:        929      28
 
EarI CTCTTCn'nnn_

Cuts at:      0       1     143     209     906     957 
   Size:          1     142      66     697      51

  Fragments arranged by size: 

                 697     142      66      51       1
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     887     957 
   Size:         25     862      70

  Fragments arranged by size: 

                 862      70      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     827     957 
   Size:        827     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     220     957 
   Size:        220     737
 
EcoRI G'AATT_C

Cuts at:      0     753     957 
   Size:        753     204
 
EcoRV GAT'ATC

Cuts at:      0     301     957 
   Size:        301     656
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     347     957 
   Size:        347     610
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     290     322     528     560     957 
   Size:        290      32     206      32     397

  Fragments arranged by size: 

                 397     290     206      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     160     957 
   Size:         48     112     797

  Fragments arranged by size: 

                 797     112      48
 
MspA1I CmG'CkG

Cuts at:      0     314     957 
   Size:        314     643
 
NdeI CA'TA_TG

Cuts at:      0     886     957 
   Size:        886      71
 
PpuMI rG'GwC_Cy

Cuts at:      0     220     957 
   Size:        220     737
 
PvuII CAG'CTG

Cuts at:      0     314     957 
   Size:        314     643
 
SfcI C'TryA_G

Cuts at:      0     440     758     824     957 
   Size:        440     318      66     133

  Fragments arranged by size: 

                 440     318     133      66
 
SmlI C'TyrA_G

Cuts at:      0     532     957 
   Size:        532     425
 
TatI w'GTAC_w

Cuts at:      0     732     957 
   Size:        732     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     312     344     645     677     957 
   Size:        312      32     301      32     280

  Fragments arranged by size: 

                 312     301     280      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     859     957 
   Size:        859      98
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApoI    BamHI     BanI    BciVI     BclI
    BglII    BpuEI    BseRI    BseYI     BsgI    BsmBI    BspMI   BstEII
    BstYI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I     MmeI   MspA1I     NdeI    PpuMI    PvuII     SfcI
     SmlI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI