Restriction map for
pFN21AE6378
(Linear) (Six-base) MAPSORT of: KIEE6378.seq Check: 6485 from: 1 to: 921
KIEE6378.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 737 921
Size: 737 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 921
Size: 253 668
AflIII A'CryG_T
Cuts at: 0 800 921
Size: 800 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 921
Size: 103 818
AlwNI CAG_nnn'CTG
Cuts at: 0 552 921
Size: 552 369
ApaLI G'TGCA_C
Cuts at: 0 219 921
Size: 219 702
ApoI r'AATT_y
Cuts at: 0 340 455 615 921
Size: 340 115 160 306
Fragments arranged by size:
340 306 160 115
AseI AT'TA_AT
Cuts at: 0 179 921
Size: 179 742
BanII G_rGCy'C
Cuts at: 0 612 921
Size: 612 309
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 921
Size: 310 611
BbvCI CC'TCA_GC
Cuts at: 0 439 921
Size: 439 482
BglII A'GATC_T
Cuts at: 0 875 921
Size: 875 46
Bme1580I G_kGCm'C
Cuts at: 0 223 921
Size: 223 698
BmrI ACTGGGnnnn_n'
Cuts at: 0 481 921
Size: 481 440
Bpu10I CC'TnA_GC
Cuts at: 0 439 883 921
Size: 439 444 38
Fragments arranged by size:
444 439 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 395 921
Size: 395 526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 848 878 921
Size: 848 30 43
Fragments arranged by size:
848 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 921
Size: 117 804
BsiHKAI G_wGCw'C
Cuts at: 0 223 612 921
Size: 223 389 309
Fragments arranged by size:
389 309 223
BsmI GAATG_Cn'
Cuts at: 0 92 921
Size: 92 829
Bsp1286I G_dGCh'C
Cuts at: 0 223 612 921
Size: 223 389 309
Fragments arranged by size:
389 309 223
BsrDI GCAATG_nn'
Cuts at: 0 683 921
Size: 683 238
BsrFI r'CCGG_y
Cuts at: 0 745 921
Size: 745 176
BsrGI T'GTAC_A
Cuts at: 0 151 921
Size: 151 770
BstYI r'GATC_y
Cuts at: 0 875 921
Size: 875 46
DraI TTT'AAA
Cuts at: 0 111 921
Size: 111 810
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 768 921
Size: 768 153
EcoICRI GAG'CTC
Cuts at: 0 610 921
Size: 610 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 921
Size: 253 668
FspI TGC'GCA
Cuts at: 0 820 921
Size: 820 101
HincII GTy'rAC
Cuts at: 0 510 921
Size: 510 411
MslI CAynn'nnrTG
Cuts at: 0 85 921
Size: 85 836
NspI r_CATG'y
Cuts at: 0 423 804 921
Size: 423 381 117
Fragments arranged by size:
423 381 117
PasI CC'CwG_GG
Cuts at: 0 894 921
Size: 894 27
PciI A'CATG_T
Cuts at: 0 800 921
Size: 800 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 921
Size: 552 369
PfoI T'CCnGG_A
Cuts at: 0 257 921
Size: 257 664
PsiI TTA'TAA
Cuts at: 0 173 293 921
Size: 173 120 628
Fragments arranged by size:
628 173 120
PstI C_TGCA'G
Cuts at: 0 656 921
Size: 656 265
SacI G_AGCT'C
Cuts at: 0 612 921
Size: 612 309
SfcI C'TryA_G
Cuts at: 0 638 652 871 921
Size: 638 14 219 50
Fragments arranged by size:
638 219 50 14
StuI AGG'CCT
Cuts at: 0 444 921
Size: 444 477
StyI C'CwwG_G
Cuts at: 0 696 921
Size: 696 225
TatI w'GTAC_w
Cuts at: 0 151 921
Size: 151 770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 921
Size: 468 453
XmnI GAAnn'nnTTC
Cuts at: 0 277 921
Size: 277 644
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaLI ApoI AseI
BanII BbsI BbvCI BglII Bme1580I BmrI Bpu10I BsaI
BsaXI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI
BstYI DraI EciI EcoICRI Eco57MI FspI HincII MslI
NspI PasI PciI PflMI PfoI PsiI PstI SacI
SfcI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EarI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI