Restriction map for
pFN21AE6374
(Linear) (Six-base) MAPSORT of: KIEE6374.seq Check: 7814 from: 1 to: 1317
KIEE6374.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 151 244 559 1317
Size: 151 93 315 758
Fragments arranged by size:
758 315 151 93
AccI GT'mk_AC
Cuts at: 0 636 1317
Size: 636 681
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1172 1317
Size: 1036 136 145
Fragments arranged by size:
1036 145 136
AflIII A'CryG_T
Cuts at: 0 424 1317
Size: 424 893
AleI CACnn'nnGTG
Cuts at: 0 363 1189 1278 1317
Size: 363 826 89 39
Fragments arranged by size:
826 363 89 39
ApaI G_GGCC'C
Cuts at: 0 121 1317
Size: 121 1196
ApoI r'AATT_y
Cuts at: 0 772 1317
Size: 772 545
AvaI C'yCGr_G
Cuts at: 0 843 1317
Size: 843 474
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 599 632 1317
Size: 599 33 685
Fragments arranged by size:
685 599 33
BamHI G'GATC_C
Cuts at: 0 1068 1317
Size: 1068 249
BanII G_rGCy'C
Cuts at: 0 121 270 850 1317
Size: 121 149 580 467
Fragments arranged by size:
580 467 149 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 581 615 1317
Size: 581 34 702
Fragments arranged by size:
702 581 34
BclI T'GATC_A
Cuts at: 0 666 1317
Size: 666 651
BfrBI ATG'CAT
Cuts at: 0 1208 1317
Size: 1208 109
BlpI GC'TnA_GC
Cuts at: 0 269 279 927 1317
Size: 269 10 648 390
Fragments arranged by size:
648 390 269 10
Bme1580I G_kGCm'C
Cuts at: 0 121 1317
Size: 121 1196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1317
Size: 679 638
Bpu10I CC'TnA_GC
Cuts at: 0 181 1317
Size: 181 1136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1317
Size: 346 971
BsaI GGTCTCn'nnnn_
Cuts at: 0 1289 1317
Size: 1289 28
BsaBI GATnn'nnATC
Cuts at: 0 1228 1317
Size: 1228 89
BsaWI w'CCGG_w
Cuts at: 0 1287 1317
Size: 1287 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 975 1005 1317
Size: 832 30 113 30 312
Fragments arranged by size:
832 312 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 100 956 996 1104 1107 1177 1317
Size: 28 72 856 40 108 3 70 140
Fragments arranged by size:
856 140 108 72 70 40 28 3
BseYI C'CCAG_C
Cuts at: 0 495 581 1317
Size: 495 86 736
Fragments arranged by size:
736 495 86
BsiHKAI G_wGCw'C
Cuts at: 0 270 1114 1317
Size: 270 844 203
Fragments arranged by size:
844 270 203
BsmI GAATG_Cn'
Cuts at: 0 10 136 1317
Size: 10 126 1181
Fragments arranged by size:
1181 126 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 901 1317
Size: 901 416
Bsp1286I G_dGCh'C
Cuts at: 0 121 270 850 1114 1317
Size: 121 149 580 264 203
Fragments arranged by size:
580 264 203 149 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 151 244 559 1317
Size: 151 93 315 758
Fragments arranged by size:
758 315 151 93
BsrDI GCAATG_nn'
Cuts at: 0 280 1317
Size: 280 1037
BsrFI r'CCGG_y
Cuts at: 0 554 1317
Size: 554 763
BsrGI T'GTAC_A
Cuts at: 0 1211 1317
Size: 1211 106
BssSI C'ACGA_G
Cuts at: 0 1119 1317
Size: 1119 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 478 1085 1317
Size: 478 607 232
Fragments arranged by size:
607 478 232
BstYI r'GATC_y
Cuts at: 0 1068 1317
Size: 1068 249
Bsu36I CC'TnA_GG
Cuts at: 0 407 1317
Size: 407 910
BtgI C'CryG_G
Cuts at: 0 121 303 1099 1317
Size: 121 182 796 218
Fragments arranged by size:
796 218 182 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1215 1317
Size: 1215 102
BtsI GCAGTG_nn'
Cuts at: 0 31 1317
Size: 31 1286
DrdI GACnn_nn'nnGTC
Cuts at: 0 862 1317
Size: 862 455
EaeI y'GGCC_r
Cuts at: 0 306 1317
Size: 306 1011
EarI CTCTTCn'nnn_
Cuts at: 0 1190 1317
Size: 1190 127
EcoICRI GAG'CTC
Cuts at: 0 268 1317
Size: 268 1049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1036 1172 1317
Size: 679 357 136 145
Fragments arranged by size:
679 357 145 136
EcoO109I rG'GnC_Cy
Cuts at: 0 117 155 661 1317
Size: 117 38 506 656
Fragments arranged by size:
656 506 117 38
HaeII r_GCGC'y
Cuts at: 0 177 1317
Size: 177 1140
HindIII A'AGCT_T
Cuts at: 0 1048 1317
Size: 1048 269
MluI A'CGCG_T
Cuts at: 0 424 1317
Size: 424 893
MscI TGG'CCA
Cuts at: 0 308 1317
Size: 308 1009
MslI CAynn'nnrTG
Cuts at: 0 290 363 1189 1278 1317
Size: 290 73 826 89 39
Fragments arranged by size:
826 290 89 73 39
MspA1I CmG'CkG
Cuts at: 0 495 853 954 1317
Size: 495 358 101 363
Fragments arranged by size:
495 363 358 101
NaeI GCC'GGC
Cuts at: 0 556 1317
Size: 556 761
NcoI C'CATG_G
Cuts at: 0 121 303 1099 1317
Size: 121 182 796 218
Fragments arranged by size:
796 218 182 121
NgoMIV G'CCGG_C
Cuts at: 0 554 1317
Size: 554 763
NsiI A_TGCA'T
Cuts at: 0 1210 1317
Size: 1210 107
NspI r_CATG'y
Cuts at: 0 227 1183 1212 1317
Size: 227 956 29 105
Fragments arranged by size:
956 227 105 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 1100 1317
Size: 1100 217
PfoI T'CCnGG_A
Cuts at: 0 67 1317
Size: 67 1250
PpuMI rG'GwC_Cy
Cuts at: 0 661 1317
Size: 661 656
PspOMI G'GGCC_C
Cuts at: 0 117 1317
Size: 117 1200
PvuII CAG'CTG
Cuts at: 0 495 954 1317
Size: 495 459 363
Fragments arranged by size:
495 459 363
SacI G_AGCT'C
Cuts at: 0 270 1317
Size: 270 1047
SmlI C'TyrA_G
Cuts at: 0 361 1317
Size: 361 956
StyI C'CwwG_G
Cuts at: 0 121 303 1099 1317
Size: 121 182 796 218
Fragments arranged by size:
796 218 182 121
TatI w'GTAC_w
Cuts at: 0 859 1211 1317
Size: 859 352 106
Fragments arranged by size:
859 352 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 693 725 975 1007 1317
Size: 693 32 250 32 310
Fragments arranged by size:
693 310 250 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1317
Size: 555 762
XmnI GAAnn'nnTTC
Cuts at: 0 323 1317
Size: 323 994
Enzymes that do cut:
AarI AccI AcuI AflIII AleI ApaI ApoI AvaI
BaeI BamHI BanII BcgI BclI BfrBI BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HindIII
MluI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PflMI PfoI PpuMI PspOMI PvuII SacI SmlI
StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BanI
BbeI BbsI BbvCI BciVI BglI BglII BmgBI BmrI
BmtI BplI BsaAI BsaHI BsgI BsiEI BsiWI BspEI
BspHI BsrBI BssHII BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HpaI KasI
KpnI MfeI MmeI NarI NdeI NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI ZraI