Restriction map for
pFN21AE6372
(Linear) (Six-base) MAPSORT of: KIEE6372.seq Check: 8580 from: 1 to: 1071
KIEE6372.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AclI AA'CG_TT
Cuts at: 0 503 1071
Size: 503 568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1071
Size: 1012 59
AfeI AGC'GCT
Cuts at: 0 670 1071
Size: 670 401
AhdI GACnn_n'nnGTC
Cuts at: 0 413 1071
Size: 413 658
AlwNI CAG_nnn'CTG
Cuts at: 0 976 1071
Size: 976 95
AvaI C'yCGr_G
Cuts at: 0 128 606 1071
Size: 128 478 465
Fragments arranged by size:
478 465 128
BamHI G'GATC_C
Cuts at: 0 282 1071
Size: 282 789
BanI G'GyrC_C
Cuts at: 0 141 1071
Size: 141 930
BanII G_rGCy'C
Cuts at: 0 30 1071
Size: 30 1041
BbsI GAAGACnn'nnnn_
Cuts at: 0 568 1071
Size: 568 503
BciVI GTATCCnnnnn_n'
Cuts at: 0 709 1071
Size: 709 362
BclI T'GATC_A
Cuts at: 0 377 464 1071
Size: 377 87 607
Fragments arranged by size:
607 377 87
BglI GCCn_nnn'nGGC
Cuts at: 0 367 548 1071
Size: 367 181 523
Fragments arranged by size:
523 367 181
Bme1580I G_kGCm'C
Cuts at: 0 146 1071
Size: 146 925
BmgBI CAC'GTC
Cuts at: 0 954 1071
Size: 954 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 541 718 1071
Size: 476 65 177 353
Fragments arranged by size:
476 353 177 65
Bpu10I CC'TnA_GC
Cuts at: 0 776 1071
Size: 776 295
BsaHI Gr'CG_yC
Cuts at: 0 1004 1071
Size: 1004 67
BsaWI w'CCGG_w
Cuts at: 0 14 69 755 1071
Size: 14 55 686 316
Fragments arranged by size:
686 316 55 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 776 806 1071
Size: 776 30 265
Fragments arranged by size:
776 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 169 172 184 187 199 208 220
Size: 121 48 3 12 3 12 9 12
Cuts at: 220 1071
Size: 851
Fragments arranged by size:
851 121 48 12 12 12 9 3
3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1071
Size: 1000 71
Bsp1286I G_dGCh'C
Cuts at: 0 30 146 1071
Size: 30 116 925
Fragments arranged by size:
925 116 30
BspEI T'CCGG_A
Cuts at: 0 69 1071
Size: 69 1002
BspHI T'CATG_A
Cuts at: 0 263 1071
Size: 263 808
BsrBI CCG'CTC
Cuts at: 0 441 1071
Size: 441 630
BsrDI GCAATG_nn'
Cuts at: 0 243 639 944 1071
Size: 243 396 305 127
Fragments arranged by size:
396 305 243 127
BsrGI T'GTAC_A
Cuts at: 0 392 1071
Size: 392 679
BstAPI GCAn_nnn'nTGC
Cuts at: 0 255 1071
Size: 255 816
BstYI r'GATC_y
Cuts at: 0 282 786 1071
Size: 282 504 285
Fragments arranged by size:
504 285 282
BtsI GCAGTG_nn'
Cuts at: 0 977 988 1071
Size: 977 11 83
Fragments arranged by size:
977 83 11
EarI CTCTTCn'nnn_
Cuts at: 0 98 116 161 176 185 206 1071
Size: 98 18 45 15 9 21 865
Fragments arranged by size:
865 98 45 21 18 15 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 541 718 1012 1071
Size: 476 65 177 294 59
Fragments arranged by size:
476 294 177 65 59
EcoO109I rG'GnC_Cy
Cuts at: 0 702 1071
Size: 702 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 1071
Size: 690 381
HaeII r_GCGC'y
Cuts at: 0 672 1071
Size: 672 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 399 431 449 481 823 855 1071
Size: 399 32 18 32 342 32 216
Fragments arranged by size:
399 342 216 32 32 32 18
HincII GTy'rAC
Cuts at: 0 915 1071
Size: 915 156
HpaI GTT'AAC
Cuts at: 0 915 1071
Size: 915 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1071
Size: 304 767
NspI r_CATG'y
Cuts at: 0 393 1071
Size: 393 678
PflMI CCAn_nnn'nTGG
Cuts at: 0 712 1071
Size: 712 359
PfoI T'CCnGG_A
Cuts at: 0 453 1071
Size: 453 618
PpuMI rG'GwC_Cy
Cuts at: 0 702 1071
Size: 702 369
PsiI TTA'TAA
Cuts at: 0 566 1071
Size: 566 505
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 73 105 1071
Size: 73 32 966
Fragments arranged by size:
966 73 32
ScaI AGT'ACT
Cuts at: 0 616 1071
Size: 616 455
SfcI C'TryA_G
Cuts at: 0 54 1071
Size: 54 1017
SmaI CCC'GGG
Cuts at: 0 608 1071
Size: 608 463
StuI AGG'CCT
Cuts at: 0 655 1071
Size: 655 416
StyI C'CwwG_G
Cuts at: 0 365 929 1071
Size: 365 564 142
Fragments arranged by size:
564 365 142
TatI w'GTAC_w
Cuts at: 0 392 614 1071
Size: 392 222 457
Fragments arranged by size:
457 392 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 1071
Size: 776 32 263
Fragments arranged by size:
776 263 32
XmaI C'CCGG_G
Cuts at: 0 606 1071
Size: 606 465
Enzymes that do cut:
AclI AcuI AfeI AhdI AlwNI AvaI BamHI BanI
BanII BbsI BciVI BclI BglI Bme1580I BmgBI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BsgI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrGI BstAPI BstYI BtsI EarI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI MmeI
NspI PflMI PfoI PpuMI PsiI PsrI ScaI SfcI
SmaI StuI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BglII BlpI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspMI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
KasI KpnI MfeI MluI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XhoI XmnI ZraI