Restriction map for
pFN21AE6371
(Linear) (Six-base) MAPSORT of: KIEE6371.seq Check: 6085 from: 1 to: 1269
KIEE6371.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
Acc65I G'GTAC_C
Cuts at: 0 1157 1269
Size: 1157 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 101 133 1269
Size: 101 32 1136
Fragments arranged by size:
1136 101 32
ApoI r'AATT_y
Cuts at: 0 295 1269
Size: 295 974
AseI AT'TA_AT
Cuts at: 0 665 1269
Size: 665 604
BamHI G'GATC_C
Cuts at: 0 387 1269
Size: 387 882
BanI G'GyrC_C
Cuts at: 0 1157 1269
Size: 1157 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 1269
Size: 151 1118
BfrBI ATG'CAT
Cuts at: 0 268 901 1102 1269
Size: 268 633 201 167
Fragments arranged by size:
633 268 201 167
BmtI G_CTAG'C
Cuts at: 0 623 1269
Size: 623 646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 707 907 1269
Size: 707 200 362
Fragments arranged by size:
707 362 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1269
Size: 1139 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1269
Size: 1019 250
BsaI GGTCTCn'nnnn_
Cuts at: 0 781 1269
Size: 781 488
BsaHI Gr'CG_yC
Cuts at: 0 1049 1269
Size: 1049 220
BsaWI w'CCGG_w
Cuts at: 0 779 1269
Size: 779 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 761 791 1269
Size: 761 30 478
Fragments arranged by size:
761 478 30
BseYI C'CCAG_C
Cuts at: 0 1178 1269
Size: 1178 91
BsiHKAI G_wGCw'C
Cuts at: 0 954 1269
Size: 954 315
Bsp1286I G_dGCh'C
Cuts at: 0 954 1269
Size: 954 315
BspEI T'CCGG_A
Cuts at: 0 779 1269
Size: 779 490
BsrDI GCAATG_nn'
Cuts at: 0 525 1032 1269
Size: 525 507 237
Fragments arranged by size:
525 507 237
BstBI TT'CG_AA
Cuts at: 0 760 1269
Size: 760 509
BstYI r'GATC_y
Cuts at: 0 387 465 1269
Size: 387 78 804
Fragments arranged by size:
804 387 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 628 1269
Size: 628 641
BtsI GCAGTG_nn'
Cuts at: 0 104 634 1269
Size: 104 530 635
Fragments arranged by size:
635 530 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 660 695 1269
Size: 660 35 574
Fragments arranged by size:
660 574 35
EaeI y'GGCC_r
Cuts at: 0 10 1269
Size: 10 1259
EarI CTCTTCn'nnn_
Cuts at: 0 590 1269
Size: 590 679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1269
Size: 1139 130
EcoO109I rG'GnC_Cy
Cuts at: 0 451 1269
Size: 451 818
EcoRV GAT'ATC
Cuts at: 0 1152 1269
Size: 1152 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 457 489 1269
Size: 120 32 305 32 780
Fragments arranged by size:
780 305 120 32 32
HincII GTy'rAC
Cuts at: 0 328 1269
Size: 328 941
HindIII A'AGCT_T
Cuts at: 0 1037 1269
Size: 1037 232
KpnI G_GTAC'C
Cuts at: 0 1161 1269
Size: 1161 108
MslI CAynn'nnrTG
Cuts at: 0 647 824 1269
Size: 647 177 445
Fragments arranged by size:
647 445 177
NdeI CA'TA_TG
Cuts at: 0 353 1269
Size: 353 916
NheI G'CTAG_C
Cuts at: 0 619 1269
Size: 619 650
NsiI A_TGCA'T
Cuts at: 0 270 903 1104 1269
Size: 270 633 201 165
Fragments arranged by size:
633 270 201 165
NspI r_CATG'y
Cuts at: 0 1106 1269
Size: 1106 163
PacI TTA_AT'TAA
Cuts at: 0 665 1269
Size: 665 604
PasI CC'CwG_GG
Cuts at: 0 53 1269
Size: 53 1216
PpuMI rG'GwC_Cy
Cuts at: 0 451 1269
Size: 451 818
PsiI TTA'TAA
Cuts at: 0 275 1269
Size: 275 994
PstI C_TGCA'G
Cuts at: 0 629 1269
Size: 629 640
SexAI A'CCwGG_T
Cuts at: 0 918 1269
Size: 918 351
SfcI C'TryA_G
Cuts at: 0 159 398 446 625 1269
Size: 159 239 48 179 644
Fragments arranged by size:
644 239 179 159 48
SmlI C'TyrA_G
Cuts at: 0 1034 1269
Size: 1034 235
SphI G_CATG'C
Cuts at: 0 1106 1269
Size: 1106 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 827 1269
Size: 827 442
TatI w'GTAC_w
Cuts at: 0 876 1269
Size: 876 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 564 596 1269
Size: 564 32 673
Fragments arranged by size:
673 564 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1114 1269
Size: 1114 155
Enzymes that do cut:
Acc65I AloI ApoI AseI BamHI BanI BbsI BfrBI
BmtI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseYI BsiHKAI Bsp1286I BspEI BsrDI BstBI BstYI BtgZI
BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I
HincII HindIII KpnI MslI NdeI NheI NsiI NspI
PacI PasI PpuMI PsiI PstI SexAI SfcI SmlI
SphI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
Bpu10I BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HpaI KasI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI