Restriction map for
pFN21AE6370
(Linear) (Six-base) MAPSORT of: KIEE6370.seq Check: 3320 from: 1 to: 978
KIEE6370.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 653 978
Size: 653 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 978
Size: 487 491
AflIII A'CryG_T
Cuts at: 0 335 659 978
Size: 335 324 319
Fragments arranged by size:
335 324 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 787 978
Size: 787 191
AlwNI CAG_nnn'CTG
Cuts at: 0 907 978
Size: 907 71
ApaI G_GGCC'C
Cuts at: 0 28 38 742 932 978
Size: 28 10 704 190 46
Fragments arranged by size:
704 190 46 28 10
ApaLI G'TGCA_C
Cuts at: 0 229 978
Size: 229 749
ApoI r'AATT_y
Cuts at: 0 754 978
Size: 754 224
AvaI C'yCGr_G
Cuts at: 0 31 83 199 978
Size: 31 52 116 779
Fragments arranged by size:
779 116 52 31
BanI G'GyrC_C
Cuts at: 0 638 978
Size: 638 340
BanII G_rGCy'C
Cuts at: 0 28 38 635 742 932 978
Size: 28 10 597 107 190 46
Fragments arranged by size:
597 190 107 46 28 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 475 978
Size: 475 503
BglI GCCn_nnn'nGGC
Cuts at: 0 958 978
Size: 958 20
BlpI GC'TnA_GC
Cuts at: 0 896 978
Size: 896 82
Bme1580I G_kGCm'C
Cuts at: 0 28 38 233 742 932 978
Size: 28 10 195 509 190 46
Fragments arranged by size:
509 195 190 46 28 10
BmgBI CAC'GTC
Cuts at: 0 249 450 978
Size: 249 201 528
Fragments arranged by size:
528 249 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 375 978
Size: 288 87 603
Fragments arranged by size:
603 288 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 978
Size: 747 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 649 845 902 978
Size: 503 146 196 57 76
Fragments arranged by size:
503 196 146 76 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 978
Size: 580 398
BsaAI yAC'GTr
Cuts at: 0 342 978
Size: 342 636
BsaHI Gr'CG_yC
Cuts at: 0 188 978
Size: 188 790
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 434 464 622 652 873 903 978
Size: 434 30 158 30 221 30 75
Fragments arranged by size:
434 221 158 75 30 30 30
BseYI C'CCAG_C
Cuts at: 0 160 548 691 791 978
Size: 160 388 143 100 187
Fragments arranged by size:
388 187 160 143 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 481 631 978
Size: 394 87 150 347
Fragments arranged by size:
394 347 150 87
BsiHKAI G_wGCw'C
Cuts at: 0 233 500 978
Size: 233 267 478
Fragments arranged by size:
478 267 233
BsmI GAATG_Cn'
Cuts at: 0 711 978
Size: 711 267
Bsp1286I G_dGCh'C
Cuts at: 0 28 38 233 500 635 742 932 978
Size: 28 10 195 267 135 107 190 46
Fragments arranged by size:
267 195 190 135 107 46 28 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 653 978
Size: 210 443 325
Fragments arranged by size:
443 325 210
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 978
Size: 609 369
Bsu36I CC'TnA_GG
Cuts at: 0 358 978
Size: 358 620
BtgI C'CryG_G
Cuts at: 0 214 978
Size: 214 764
BtsI GCAGTG_nn'
Cuts at: 0 297 978
Size: 297 681
DraIII CAC_nnn'GTG
Cuts at: 0 383 978
Size: 383 595
EaeI y'GGCC_r
Cuts at: 0 211 950 978
Size: 211 739 28
Fragments arranged by size:
739 211 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 503 649 845 902 978
Size: 487 16 146 196 57 76
Fragments arranged by size:
487 196 146 76 57 16
EcoO109I rG'GnC_Cy
Cuts at: 0 35 738 928 978
Size: 35 703 190 50
Fragments arranged by size:
703 190 50 35
EcoRI G'AATT_C
Cuts at: 0 754 978
Size: 754 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 547 978
Size: 547 431
FspI TGC'GCA
Cuts at: 0 296 978
Size: 296 682
MfeI C'AATT_G
Cuts at: 0 543 978
Size: 543 435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 978
Size: 833 145
MscI TGG'CCA
Cuts at: 0 952 978
Size: 952 26
MspA1I CmG'CkG
Cuts at: 0 292 465 621 767 978
Size: 292 173 156 146 211
Fragments arranged by size:
292 211 173 156 146
NdeI CA'TA_TG
Cuts at: 0 420 978
Size: 420 558
NspI r_CATG'y
Cuts at: 0 339 663 684 978
Size: 339 324 21 294
Fragments arranged by size:
339 324 294 21
PasI CC'CwG_GG
Cuts at: 0 45 978
Size: 45 933
PciI A'CATG_T
Cuts at: 0 335 659 978
Size: 335 324 319
Fragments arranged by size:
335 324 319
PflMI CCAn_nnn'nTGG
Cuts at: 0 548 978
Size: 548 430
PfoI T'CCnGG_A
Cuts at: 0 626 978
Size: 626 352
PshAI GACnn'nnGTC
Cuts at: 0 660 978
Size: 660 318
PspOMI G'GGCC_C
Cuts at: 0 24 34 738 928 978
Size: 24 10 704 190 50
Fragments arranged by size:
704 190 50 24 10
PstI C_TGCA'G
Cuts at: 0 605 978
Size: 605 373
PvuII CAG'CTG
Cuts at: 0 292 465 767 978
Size: 292 173 302 211
Fragments arranged by size:
302 292 211 173
ScaI AGT'ACT
Cuts at: 0 277 978
Size: 277 701
SfcI C'TryA_G
Cuts at: 0 601 978
Size: 601 377
SmaI CCC'GGG
Cuts at: 0 85 978
Size: 85 893
SnaBI TAC'GTA
Cuts at: 0 342 978
Size: 342 636
SpeI A'CTAG_T
Cuts at: 0 567 978
Size: 567 411
StuI AGG'CCT
Cuts at: 0 242 347 978
Size: 242 105 631
Fragments arranged by size:
631 242 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 394 978
Size: 270 124 584
Fragments arranged by size:
584 270 124
TatI w'GTAC_w
Cuts at: 0 275 978
Size: 275 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 622 654 873 905 978
Size: 434 32 156 32 219 32 73
Fragments arranged by size:
434 219 156 73 32 32 32
XmaI C'CCGG_G
Cuts at: 0 83 978
Size: 83 895
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaAI BsaHI BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BstAPI Bsu36I BtgI
BtsI DraIII EaeI Eco57MI EcoO109I EcoRI FalI FspI
MfeI MmeI MscI MspA1I NdeI NspI PasI PciI
PflMI PfoI PshAI PspOMI PstI PvuII ScaI SfcI
SmaI SnaBI SpeI StuI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
Bpu10I BpuEI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRV
FseI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MslI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SphI SrfI SspI StyI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI