Restriction map for
pFN21AE6369
(Linear) (Six-base) MAPSORT of: KIEE6369.seq Check: 8928 from: 1 to: 1044
KIEE6369.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 458 747 1044
Size: 458 289 297
Fragments arranged by size:
458 297 289
Acc65I G'GTAC_C
Cuts at: 0 60 1044
Size: 60 984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 1044
Size: 54 990
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 1044
Size: 681 32 331
Fragments arranged by size:
681 331 32
ApoI r'AATT_y
Cuts at: 0 169 962 1044
Size: 169 793 82
Fragments arranged by size:
793 169 82
BanI G'GyrC_C
Cuts at: 0 60 601 761 1044
Size: 60 541 160 283
Fragments arranged by size:
541 283 160 60
BanII G_rGCy'C
Cuts at: 0 848 1044
Size: 848 196
BbeI G_GCGC'C
Cuts at: 0 605 1044
Size: 605 439
Bme1580I G_kGCm'C
Cuts at: 0 182 1044
Size: 182 862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 341 1044
Size: 268 73 703
Fragments arranged by size:
703 268 73
Bpu10I CC'TnA_GC
Cuts at: 0 373 1044
Size: 373 671
BsaHI Gr'CG_yC
Cuts at: 0 602 1044
Size: 602 442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 982 1012 1044
Size: 982 30 32
Fragments arranged by size:
982 32 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 856 1044
Size: 856 188
BsiEI CG_ry'CG
Cuts at: 0 193 1044
Size: 193 851
BsiHKAI G_wGCw'C
Cuts at: 0 302 620 848 1044
Size: 302 318 228 196
Fragments arranged by size:
318 302 228 196
Bsp1286I G_dGCh'C
Cuts at: 0 182 302 620 848 1044
Size: 182 120 318 228 196
Fragments arranged by size:
318 228 196 182 120
BsrBI CCG'CTC
Cuts at: 0 282 870 1044
Size: 282 588 174
Fragments arranged by size:
588 282 174
BsrDI GCAATG_nn'
Cuts at: 0 662 1044
Size: 662 382
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 342 1044
Size: 123 219 702
Fragments arranged by size:
702 219 123
Bsu36I CC'TnA_GG
Cuts at: 0 839 1044
Size: 839 205
BtgI C'CryG_G
Cuts at: 0 143 193 1044
Size: 143 50 851
Fragments arranged by size:
851 143 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 579 1044
Size: 579 465
BtsI GCAGTG_nn'
Cuts at: 0 400 1044
Size: 400 644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 387 422 1044
Size: 387 35 622
Fragments arranged by size:
622 387 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 376 1044
Size: 376 668
EaeI y'GGCC_r
Cuts at: 0 190 1044
Size: 190 854
EagI C'GGCC_G
Cuts at: 0 190 1044
Size: 190 854
EcoICRI GAG'CTC
Cuts at: 0 846 1044
Size: 846 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 341 1044
Size: 268 73 703
Fragments arranged by size:
703 268 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 756 1044
Size: 756 288
HaeII r_GCGC'y
Cuts at: 0 605 656 1044
Size: 605 51 388
Fragments arranged by size:
605 388 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 982 1014 1044
Size: 651 32 299 32 30
Fragments arranged by size:
651 299 32 32 30
HincII GTy'rAC
Cuts at: 0 459 1044
Size: 459 585
KasI G'GCGC_C
Cuts at: 0 601 1044
Size: 601 443
KpnI G_GTAC'C
Cuts at: 0 64 1044
Size: 64 980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1044
Size: 612 432
MslI CAynn'nnrTG
Cuts at: 0 535 625 1044
Size: 535 90 419
Fragments arranged by size:
535 419 90
MspA1I CmG'CkG
Cuts at: 0 17 426 1044
Size: 17 409 618
Fragments arranged by size:
618 409 17
NarI GG'CG_CC
Cuts at: 0 602 1044
Size: 602 442
NcoI C'CATG_G
Cuts at: 0 143 1044
Size: 143 901
NspI r_CATG'y
Cuts at: 0 395 1044
Size: 395 649
PasI CC'CwG_GG
Cuts at: 0 717 1044
Size: 717 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 452 677 1044
Size: 452 225 367
Fragments arranged by size:
452 367 225
PsiI TTA'TAA
Cuts at: 0 1010 1044
Size: 1010 34
PvuII CAG'CTG
Cuts at: 0 17 1044
Size: 17 1027
SacI G_AGCT'C
Cuts at: 0 848 1044
Size: 848 196
SalI G'TCGA_C
Cuts at: 0 457 1044
Size: 457 587
SfcI C'TryA_G
Cuts at: 0 876 1044
Size: 876 168
SfoI GGC'GCC
Cuts at: 0 603 1044
Size: 603 441
SspI AAT'ATT
Cuts at: 0 641 695 908 1044
Size: 641 54 213 136
Fragments arranged by size:
641 213 136 54
StyI C'CwwG_G
Cuts at: 0 128 143 1044
Size: 128 15 901
Fragments arranged by size:
901 128 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 140 172 908 940 1044
Size: 140 32 736 32 104
Fragments arranged by size:
736 140 104 32 32
Enzymes that do cut:
AccI Acc65I AlfI AloI ApoI BanI BanII BbeI
Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BsiEI BsiHKAI
Bsp1286I BsrBI BsrDI BstXI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EcoICRI Eco57MI EcoNI HaeII
Hin4I HincII KasI KpnI MmeI MslI MspA1I NarI
NcoI NspI PasI PflMI PsiI PvuII SacI SalI
SfcI SfoI SspI StyI TstI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI
DraI DraIII EarI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI