Restriction map for
pFN21AE6368
(Linear) (Six-base) MAPSORT of: KIEE6368.seq Check: 3458 from: 1 to: 1083
KIEE6368.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 50 1083
Size: 50 1033
Acc65I G'GTAC_C
Cuts at: 0 347 1083
Size: 347 736
AclI AA'CG_TT
Cuts at: 0 766 1083
Size: 766 317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 998 1083
Size: 277 721 85
Fragments arranged by size:
721 277 85
AgeI A'CCGG_T
Cuts at: 0 350 1083
Size: 350 733
AhdI GACnn_n'nnGTC
Cuts at: 0 403 1083
Size: 403 680
ApoI r'AATT_y
Cuts at: 0 144 281 1011 1083
Size: 144 137 730 72
Fragments arranged by size:
730 144 137 72
AvaI C'yCGr_G
Cuts at: 0 234 1083
Size: 234 849
BamHI G'GATC_C
Cuts at: 0 238 1083
Size: 238 845
BanI G'GyrC_C
Cuts at: 0 31 175 347 700 1083
Size: 31 144 172 353 383
Fragments arranged by size:
383 353 172 144 31
BanII G_rGCy'C
Cuts at: 0 756 1083
Size: 756 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 1083
Size: 18 1065
BbvCI CC'TCA_GC
Cuts at: 0 99 1083
Size: 99 984
Bme1580I G_kGCm'C
Cuts at: 0 34 1083
Size: 34 1049
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 1083
Size: 312 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 710 1083
Size: 562 148 373
Fragments arranged by size:
562 373 148
Bpu10I CC'TnA_GC
Cuts at: 0 99 1083
Size: 99 984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 611 835 1083
Size: 348 263 224 248
Fragments arranged by size:
348 263 248 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 626 650 1083
Size: 626 24 433
Fragments arranged by size:
626 433 24
BsaWI w'CCGG_w
Cuts at: 0 350 1083
Size: 350 733
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 859 889 1083
Size: 409 30 420 30 194
Fragments arranged by size:
420 409 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 614 1083
Size: 127 487 469
Fragments arranged by size:
487 469 127
BsiHKAI G_wGCw'C
Cuts at: 0 756 1083
Size: 756 327
BsmBI CGTCTCn'nnnn_
Cuts at: 0 971 1083
Size: 971 112
Bsp1286I G_dGCh'C
Cuts at: 0 34 756 1083
Size: 34 722 327
Fragments arranged by size:
722 327 34
BspHI T'CATG_A
Cuts at: 0 834 1083
Size: 834 249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 1083
Size: 50 1033
BsrDI GCAATG_nn'
Cuts at: 0 214 500 1083
Size: 214 286 583
Fragments arranged by size:
583 286 214
BsrFI r'CCGG_y
Cuts at: 0 17 350 1083
Size: 17 333 733
Fragments arranged by size:
733 333 17
BssSI C'ACGA_G
Cuts at: 0 512 1083
Size: 512 571
BstAPI GCAn_nnn'nTGC
Cuts at: 0 273 1083
Size: 273 810
BstBI TT'CG_AA
Cuts at: 0 803 1083
Size: 803 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 715 1083
Size: 715 368
BstYI r'GATC_y
Cuts at: 0 238 1083
Size: 238 845
BtgI C'CryG_G
Cuts at: 0 992 1083
Size: 992 91
BtsI GCAGTG_nn'
Cuts at: 0 386 1083
Size: 386 697
EaeI y'GGCC_r
Cuts at: 0 56 106 1083
Size: 56 50 977
Fragments arranged by size:
977 56 50
EarI CTCTTCn'nnn_
Cuts at: 0 161 596 930 1083
Size: 161 435 334 153
Fragments arranged by size:
435 334 161 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 23 1083
Size: 23 1060
EcoICRI GAG'CTC
Cuts at: 0 754 1083
Size: 754 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 562 710 998 1083
Size: 277 285 148 288 85
Fragments arranged by size:
288 285 277 148 85
EcoRI G'AATT_C
Cuts at: 0 144 281 1083
Size: 144 137 802
Fragments arranged by size:
802 144 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 389 421 738 770 951 983 1083
Size: 389 32 317 32 181 32 100
Fragments arranged by size:
389 317 181 100 32 32 32
KpnI G_GTAC'C
Cuts at: 0 351 1083
Size: 351 732
MfeI C'AATT_G
Cuts at: 0 818 1083
Size: 818 265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1046 1083
Size: 482 564 37
Fragments arranged by size:
564 482 37
MscI TGG'CCA
Cuts at: 0 108 1083
Size: 108 975
MspA1I CmG'CkG
Cuts at: 0 582 1083
Size: 582 501
NcoI C'CATG_G
Cuts at: 0 992 1083
Size: 992 91
PasI CC'CwG_GG
Cuts at: 0 68 1083
Size: 68 1015
PvuII CAG'CTG
Cuts at: 0 582 1083
Size: 582 501
SacI G_AGCT'C
Cuts at: 0 756 1083
Size: 756 327
SapI GCTCTTCn'nnn_
Cuts at: 0 161 1083
Size: 161 922
SgrAI Cr'CCGG_yG
Cuts at: 0 17 1083
Size: 17 1066
SmaI CCC'GGG
Cuts at: 0 236 1083
Size: 236 847
SmlI C'TyrA_G
Cuts at: 0 327 626 850 1083
Size: 327 299 224 233
Fragments arranged by size:
327 299 233 224
StyI C'CwwG_G
Cuts at: 0 992 1083
Size: 992 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 698 1083
Size: 698 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 366 398 409 441 1083
Size: 366 32 11 32 642
Fragments arranged by size:
642 366 32 32 11
Tth111I GACn'n_nGTC
Cuts at: 0 304 1083
Size: 304 779
XmaI C'CCGG_G
Cuts at: 0 234 1083
Size: 234 849
XmnI GAAnn'nnTTC
Cuts at: 0 809 1073 1083
Size: 809 264 10
Fragments arranged by size:
809 264 10
Enzymes that do cut:
AarI Acc65I AclI AcuI AgeI AhdI ApoI AvaI
BamHI BanI BanII BbsI BbvCI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoRI Hin4I KpnI MfeI MmeI MscI MspA1I
NcoI PasI PvuII SacI SapI SgrAI SmaI SmlI
StyI TaqII TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsaHI BseYI BsgI
BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MluI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XcmI XhoI ZraI