(Linear) (Six-base) MAPSORT of: KIEE6367.seq  Check: 3289  from: 1  to: 1080



KIEE6367.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AclI AA'CG_TT

Cuts at:      0     102    1080 
   Size:        102     978
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     487     512     888    1080 
   Size:        204     283      25     376     192

  Fragments arranged by size: 

                 376     283     204     192      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391    1080 
   Size:        391     689
 
ApoI r'AATT_y

Cuts at:      0     446     552    1005    1080 
   Size:        446     106     453      75

  Fragments arranged by size: 

                 453     446     106      75
 
BanI G'GyrC_C

Cuts at:      0     848    1080 
   Size:        848     232
 
BbeI G_GCGC'C

Cuts at:      0     852    1080 
   Size:        852     228
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      52    1080 
   Size:         52    1028
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     391    1080 
   Size:        391     689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962     989    1080 
   Size:        962      27      91

  Fragments arranged by size: 

                 962      91      27
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     646    1080 
   Size:        384     262     434

  Fragments arranged by size: 

                 434     384     262
 
BsaHI Gr'CG_yC

Cuts at:      0     849    1080 
   Size:        849     231
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     231     261    1080 
   Size:        231      30     819

  Fragments arranged by size: 

                 819     231      30
 
BseYI C'CCAG_C

Cuts at:      0      91    1080 
   Size:         91     989
 
BsmI GAATG_Cn'

Cuts at:      0     159    1080 
   Size:        159     921
 
BsrFI r'CCGG_y

Cuts at:      0     845    1080 
   Size:        845     235
 
BssSI C'ACGA_G

Cuts at:      0     546     991    1080 
   Size:        546     445      89

  Fragments arranged by size: 

                 546     445      89
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     885    1080 
   Size:        885     195
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     173     831    1080 
   Size:        173     658     249

  Fragments arranged by size: 

                 658     249     173
 
Bsu36I CC'TnA_GG

Cuts at:      0     191    1080 
   Size:        191     889
 
BtgI C'CryG_G

Cuts at:      0    1019    1080 
   Size:       1019      61
 
BtsI GCAGTG_nn'

Cuts at:      0     624     789     895    1080 
   Size:        624     165     106     185

  Fragments arranged by size: 

                 624     185     165     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     487     512     888     962     989    1080 
   Size:        204     283      25     376      74      27      91

  Fragments arranged by size: 

                 376     283     204      91      74      27      25
 
HaeII r_GCGC'y

Cuts at:      0     852    1080 
   Size:        852     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     231     263     937     969    1080 
   Size:        231      32     674      32     111

  Fragments arranged by size: 

                 674     231     111      32      32
 
KasI G'GCGC_C

Cuts at:      0     848    1080 
   Size:        848     232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1080 
   Size:        848     232
 
NarI GG'CG_CC

Cuts at:      0     849    1080 
   Size:        849     231
 
NspI r_CATG'y

Cuts at:      0     636     809    1080 
   Size:        636     173     271

  Fragments arranged by size: 

                 636     271     173
 
SfoI GGC'GCC

Cuts at:      0     850    1080 
   Size:        850     230
 
SmlI C'TyrA_G

Cuts at:      0     363     661    1080 
   Size:        363     298     419

  Fragments arranged by size: 

                 419     363     298
 
SpeI A'CTAG_T

Cuts at:      0     721    1080 
   Size:        721     359
 
SphI G_CATG'C

Cuts at:      0     636     809    1080 
   Size:        636     173     271

  Fragments arranged by size: 

                 636     271     173
 
StuI AGG'CCT

Cuts at:      0     571    1080 
   Size:        571     509
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     545    1080 
   Size:        545     535
 
XmnI GAAnn'nnTTC

Cuts at:      0     386    1080 
   Size:        386     694
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     BanI     BbeI     BmrI     BplI
     BpmI    BpuEI    BsaHI    BsaXI    BseYI     BsmI    BsrFI    BssSI
   BstAPI    BstXI   Bsu36I     BtgI     BtsI  Eco57MI    HaeII    Hin4I
     KasI     MmeI     NarI     NspI     SfoI     SmlI     SpeI     SphI
     StuI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI