(Linear) (Six-base) MAPSORT of: KIEE6366.seq  Check: 7080  from: 1  to: 1299



KIEE6366.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AccI GT'mk_AC

Cuts at:      0     755    1299 
   Size:        755     544
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1052    1299 
   Size:        665     387     247

  Fragments arranged by size: 

                 665     387     247
 
AfeI AGC'GCT

Cuts at:      0     916    1299 
   Size:        916     383
 
AflIII A'CryG_T

Cuts at:      0     489    1299 
   Size:        489     810
 
ApoI r'AATT_y

Cuts at:      0     238    1199    1299 
   Size:        238     961     100

  Fragments arranged by size: 

                 961     238     100
 
AvaI C'yCGr_G

Cuts at:      0     923    1299 
   Size:        923     376
 
BanII G_rGCy'C

Cuts at:      0     677    1299 
   Size:        677     622
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389    1299 
   Size:        389     910
 
BclI T'GATC_A

Cuts at:      0     503    1299 
   Size:        503     796
 
BfrBI ATG'CAT

Cuts at:      0    1159    1299 
   Size:       1159     140
 
BglI GCCn_nnn'nGGC

Cuts at:      0     958    1174    1299 
   Size:        958     216     125

  Fragments arranged by size: 

                 958     216     125
 
BglII A'GATC_T

Cuts at:      0      76    1299 
   Size:         76    1223
 
BsaAI yAC'GTr

Cuts at:      0     437    1299 
   Size:        437     862
 
BsaHI Gr'CG_yC

Cuts at:      0     947    1299 
   Size:        947     352
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     631     943    1299 
   Size:        631     312     356

  Fragments arranged by size: 

                 631     356     312
 
BseYI C'CCAG_C

Cuts at:      0     195    1299 
   Size:        195    1104
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     973    1299 
   Size:        973     326
 
BsmI GAATG_Cn'

Cuts at:      0      27    1299 
   Size:         27    1272
 
Bsp1286I G_dGCh'C

Cuts at:      0     677    1299 
   Size:        677     622
 
BspHI T'CATG_A

Cuts at:      0     218     725     849    1077    1089    1299 
   Size:        218     507     124     228      12     210

  Fragments arranged by size: 

                 507     228     218     210     124      12
 
BsrDI GCAATG_nn'

Cuts at:      0     365    1299 
   Size:        365     934
 
BsrFI r'CCGG_y

Cuts at:      0     943    1299 
   Size:        943     356
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1018    1252    1299 
   Size:       1018     234      47

  Fragments arranged by size: 

                1018     234      47
 
BstYI r'GATC_y

Cuts at:      0      76    1299 
   Size:         76    1223
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1016    1299 
   Size:       1016     283
 
DraI TTT'AAA

Cuts at:      0     516    1299 
   Size:        516     783
 
EaeI y'GGCC_r

Cuts at:      0     266     819    1299 
   Size:        266     553     480

  Fragments arranged by size: 

                 553     480     266
 
EarI CTCTTCn'nnn_

Cuts at:      0     713    1299 
   Size:        713     586
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1052    1299 
   Size:        665     387     247

  Fragments arranged by size: 

                 665     387     247
 
HaeII r_GCGC'y

Cuts at:      0     918    1299 
   Size:        918     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1299 
   Size:         61      32    1206

  Fragments arranged by size: 

                1206      61      32
 
MfeI C'AATT_G

Cuts at:      0    1013    1299 
   Size:       1013     286
 
MslI CAynn'nnrTG

Cuts at:      0      95     317     344     848    1299 
   Size:         95     222      27     504     451

  Fragments arranged by size: 

                 504     451     222      95      27
 
MspA1I CmG'CkG

Cuts at:      0      18    1299 
   Size:         18    1281
 
NaeI GCC'GGC

Cuts at:      0     945    1299 
   Size:        945     354
 
NgoMIV G'CCGG_C

Cuts at:      0     943    1299 
   Size:        943     356
 
NsiI A_TGCA'T

Cuts at:      0    1161    1299 
   Size:       1161     138
 
NspI r_CATG'y

Cuts at:      0     493    1299 
   Size:        493     806
 
PciI A'CATG_T

Cuts at:      0     489    1299 
   Size:        489     810
 
PmlI CAC'GTG

Cuts at:      0     437    1299 
   Size:        437     862
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     479     511    1299 
   Size:        479      32     788

  Fragments arranged by size: 

                 788     479      32
 
PsiI TTA'TAA

Cuts at:      0    1154    1299 
   Size:       1154     145
 
PstI C_TGCA'G

Cuts at:      0     705    1299 
   Size:        705     594
 
ScaI AGT'ACT

Cuts at:      0    1056    1299 
   Size:       1056     243
 
SfcI C'TryA_G

Cuts at:      0     701    1299 
   Size:        701     598
 
StuI AGG'CCT

Cuts at:      0     464    1299 
   Size:        464     835
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     967    1299 
   Size:        967     332
 
TatI w'GTAC_w

Cuts at:      0    1054    1299 
   Size:       1054     245
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     267     299    1299 
   Size:        267      32    1000

  Fragments arranged by size: 

                1000     267      32
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     ApoI     AvaI    BanII     BbsI
     BclI    BfrBI     BglI    BglII    BsaAI    BsaHI    BseRI    BseYI
     BsgI     BsmI Bsp1286I    BspHI    BsrDI    BsrFI   BstAPI    BstYI
    BtgZI     DraI     EaeI     EarI  Eco57MI    HaeII    Hin4I     MfeI
     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PciI     PmlI
     PpiI     PsiI     PstI     ScaI     SfcI     StuI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     NarI     NcoI     NdeI
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI