Restriction map for
pFN21AE6365
(Linear) (Six-base) MAPSORT of: KIEE6365.seq Check: 863 from: 1 to: 1275
KIEE6365.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 287 1275
Size: 287 988
AccI GT'mk_AC
Cuts at: 0 308 1025 1275
Size: 308 717 250
Fragments arranged by size:
717 308 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 121 432 517 1000 1178 1275
Size: 60 61 311 85 483 178 97
Fragments arranged by size:
483 311 178 97 85 61 60
AflIII A'CryG_T
Cuts at: 0 152 260 1275
Size: 152 108 1015
Fragments arranged by size:
1015 152 108
ApaLI G'TGCA_C
Cuts at: 0 598 1275
Size: 598 677
AvaI C'yCGr_G
Cuts at: 0 593 773 1275
Size: 593 180 502
Fragments arranged by size:
593 502 180
BanII G_rGCy'C
Cuts at: 0 70 377 1275
Size: 70 307 898
Fragments arranged by size:
898 307 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 1275
Size: 684 591
BbvCI CC'TCA_GC
Cuts at: 0 173 1275
Size: 173 1102
BciVI GTATCCnnnnn_n'
Cuts at: 0 502 1275
Size: 502 773
BfrBI ATG'CAT
Cuts at: 0 294 1275
Size: 294 981
BglII A'GATC_T
Cuts at: 0 554 1275
Size: 554 721
Bme1580I G_kGCm'C
Cuts at: 0 602 653 1275
Size: 602 51 622
Fragments arranged by size:
622 602 51
BmgBI CAC'GTC
Cuts at: 0 155 263 1275
Size: 155 108 1012
Fragments arranged by size:
1012 155 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 27 46 148 285 1275
Size: 9 18 19 102 137 990
Fragments arranged by size:
990 137 102 19 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 173 371 1275
Size: 173 198 904
Fragments arranged by size:
904 198 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1275
Size: 892 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 463 469 1275
Size: 463 6 806
Fragments arranged by size:
806 463 6
BsaBI GATnn'nnATC
Cuts at: 0 919 1275
Size: 919 356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 1210 1240 1275
Size: 694 30 486 30 35
Fragments arranged by size:
694 486 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 1275
Size: 673 602
BseYI C'CCAG_C
Cuts at: 0 70 109 168 1275
Size: 70 39 59 1107
Fragments arranged by size:
1107 70 59 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1275
Size: 199 1076
BsiEI CG_ry'CG
Cuts at: 0 137 1275
Size: 137 1138
BsiHKAI G_wGCw'C
Cuts at: 0 200 377 602 1275
Size: 200 177 225 673
Fragments arranged by size:
673 225 200 177
Bsp1286I G_dGCh'C
Cuts at: 0 70 200 377 602 653 1275
Size: 70 130 177 225 51 622
Fragments arranged by size:
622 225 177 130 70 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 866 1275
Size: 287 579 409
Fragments arranged by size:
579 409 287
BsrDI GCAATG_nn'
Cuts at: 0 324 1275
Size: 324 951
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 1275
Size: 204 1071
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 1275
Size: 117 1158
BstYI r'GATC_y
Cuts at: 0 554 936 1275
Size: 554 382 339
Fragments arranged by size:
554 382 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 928 1197 1275
Size: 928 269 78
Fragments arranged by size:
928 269 78
BtsI GCAGTG_nn'
Cuts at: 0 863 1151 1275
Size: 863 288 124
Fragments arranged by size:
863 288 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 1275
Size: 336 35 904
Fragments arranged by size:
904 336 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 1275
Size: 547 728
EaeI y'GGCC_r
Cuts at: 0 134 357 1275
Size: 134 223 918
Fragments arranged by size:
918 223 134
EagI C'GGCC_G
Cuts at: 0 134 1275
Size: 134 1141
EarI CTCTTCn'nnn_
Cuts at: 0 39 1275
Size: 39 1236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 987 1275
Size: 106 881 288
Fragments arranged by size:
881 288 106
EcoICRI GAG'CTC
Cuts at: 0 375 1275
Size: 375 900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 27 46 60 121 148 285 432
Size: 9 18 19 14 61 27 137 147
Cuts at: 432 517 1000 1178 1275
Size: 85 483 178 97
Fragments arranged by size:
483 178 147 137 97 85 61 27
19 18 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 1275
Size: 754 521
EcoO109I rG'GnC_Cy
Cuts at: 0 105 1275
Size: 105 1170
EcoRV GAT'ATC
Cuts at: 0 165 798 1275
Size: 165 633 477
Fragments arranged by size:
633 477 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 1266 1275
Size: 462 804 9
Fragments arranged by size:
804 462 9
HaeII r_GCGC'y
Cuts at: 0 276 1275
Size: 276 999
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 397 429 694 726 882 914
Size: 229 32 136 32 265 32 156 32
Cuts at: 914 1275
Size: 361
Fragments arranged by size:
361 265 229 156 136 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1275
Size: 880 395
MslI CAynn'nnrTG
Cuts at: 0 686 1275
Size: 686 589
MspA1I CmG'CkG
Cuts at: 0 172 225 1275
Size: 172 53 1050
Fragments arranged by size:
1050 172 53
NsiI A_TGCA'T
Cuts at: 0 296 1275
Size: 296 979
NspI r_CATG'y
Cuts at: 0 298 1275
Size: 298 977
PasI CC'CwG_GG
Cuts at: 0 117 1275
Size: 117 1158
PfoI T'CCnGG_A
Cuts at: 0 125 740 1275
Size: 125 615 535
Fragments arranged by size:
615 535 125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 826 858 1275
Size: 826 32 417
Fragments arranged by size:
826 417 32
PpuMI rG'GwC_Cy
Cuts at: 0 105 1275
Size: 105 1170
PstI C_TGCA'G
Cuts at: 0 224 590 870 1275
Size: 224 366 280 405
Fragments arranged by size:
405 366 280 224
PvuII CAG'CTG
Cuts at: 0 172 1275
Size: 172 1103
SacI G_AGCT'C
Cuts at: 0 377 1275
Size: 377 898
SfcI C'TryA_G
Cuts at: 0 220 233 586 866 1275
Size: 220 13 353 280 409
Fragments arranged by size:
409 353 280 220 13
SmaI CCC'GGG
Cuts at: 0 595 1275
Size: 595 680
SmlI C'TyrA_G
Cuts at: 0 907 1275
Size: 907 368
StuI AGG'CCT
Cuts at: 0 22 760 1163 1275
Size: 22 738 403 112
Fragments arranged by size:
738 403 112 22
StyI C'CwwG_G
Cuts at: 0 50 283 518 1229 1275
Size: 50 233 235 711 46
Fragments arranged by size:
711 235 233 50 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 487 1275
Size: 177 310 788
Fragments arranged by size:
788 310 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1002 1034 1210 1242 1275
Size: 1002 32 176 32 33
Fragments arranged by size:
1002 176 33 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 1275
Size: 724 551
XmaI C'CCGG_G
Cuts at: 0 593 1275
Size: 593 682
XmnI GAAnn'nnTTC
Cuts at: 0 1249 1275
Size: 1249 26
Enzymes that do cut:
AarI AccI AcuI AflIII ApaLI AvaI BanII BbsI
BbvCI BciVI BfrBI BglII Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI BtgZI
BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I MmeI
MslI MspA1I NsiI NspI PasI PfoI PpiI PpuMI
PstI PvuII SacI SfcI SmaI SmlI StuI StyI
TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BclI BglI
BlpI BmrI BmtI BplI BsaAI BsaHI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI ClaI DraI
DraIII EcoRI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PflMI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI ZraI