(Linear) (Six-base) MAPSORT of: KIEE6364.seq  Check: 4670  from: 1  to: 1062



KIEE6364.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508    1062 
   Size:        508     554
 
AflIII A'CryG_T

Cuts at:      0     317    1062 
   Size:        317     745
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     810     842    1062 
   Size:        810      32     220

  Fragments arranged by size: 

                 810     220      32
 
ApaI G_GGCC'C

Cuts at:      0     852    1062 
   Size:        852     210
 
ApoI r'AATT_y

Cuts at:      0     793     808    1062 
   Size:        793      15     254

  Fragments arranged by size: 

                 793     254      15
 
AvaI C'yCGr_G

Cuts at:      0     379    1062 
   Size:        379     683
 
BanII G_rGCy'C

Cuts at:      0     363     852    1062 
   Size:        363     489     210

  Fragments arranged by size: 

                 489     363     210
 
BclI T'GATC_A

Cuts at:      0     176    1062 
   Size:        176     886
 
BglII A'GATC_T

Cuts at:      0     976    1062 
   Size:        976      86
 
Bme1580I G_kGCm'C

Cuts at:      0     852    1062 
   Size:        852     210
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576     964    1062 
   Size:        576     388      98

  Fragments arranged by size: 

                 576     388      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     392    1062 
   Size:        392     670
 
BsaAI yAC'GTr

Cuts at:      0     208     921    1062 
   Size:        208     713     141

  Fragments arranged by size: 

                 713     208     141
 
BsaWI w'CCGG_w

Cuts at:      0     516    1062 
   Size:        516     546
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     810     840    1062 
   Size:        810      30     222

  Fragments arranged by size: 

                 810     222      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     668    1030    1062 
   Size:        668     362      32

  Fragments arranged by size: 

                 668     362      32
 
BseYI C'CCAG_C

Cuts at:      0     997    1040    1062 
   Size:        997      43      22

  Fragments arranged by size: 

                 997      43      22
 
BsmI GAATG_Cn'

Cuts at:      0     958    1062 
   Size:        958     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     363     852    1062 
   Size:        363     489     210

  Fragments arranged by size: 

                 489     363     210
 
BspEI T'CCGG_A

Cuts at:      0     516    1062 
   Size:        516     546
 
BsrDI GCAATG_nn'

Cuts at:      0     920    1062 
   Size:        920     142
 
BstYI r'GATC_y

Cuts at:      0     513     976    1062 
   Size:        513     463      86

  Fragments arranged by size: 

                 513     463      86
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     893    1062 
   Size:        893     169
 
ClaI AT'CG_AT

Cuts at:      0     368    1062 
   Size:        368     694
 
DraI TTT'AAA

Cuts at:      0     764    1062 
   Size:        764     298
 
EaeI y'GGCC_r

Cuts at:      0      59     611    1062 
   Size:         59     552     451

  Fragments arranged by size: 

                 552     451      59
 
EarI CTCTTCn'nnn_

Cuts at:      0      20     827    1062 
   Size:         20     807     235

  Fragments arranged by size: 

                 807     235      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     576     964    1062 
   Size:        508      68     388      98

  Fragments arranged by size: 

                 508     388      98      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1001    1062 
   Size:       1001      61
 
HincII GTy'rAC

Cuts at:      0     669    1062 
   Size:        669     393
 
MfeI C'AATT_G

Cuts at:      0     438    1062 
   Size:        438     624
 
MscI TGG'CCA

Cuts at:      0      61     613    1062 
   Size:         61     552     449

  Fragments arranged by size: 

                 552     449      61
 
NspI r_CATG'y

Cuts at:      0     321    1062 
   Size:        321     741
 
PciI A'CATG_T

Cuts at:      0     317    1062 
   Size:        317     745
 
PmlI CAC'GTG

Cuts at:      0     921    1062 
   Size:        921     141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     810     842    1062 
   Size:        810      32     220

  Fragments arranged by size: 

                 810     220      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1001    1062 
   Size:       1001      61
 
PspOMI G'GGCC_C

Cuts at:      0     848    1062 
   Size:        848     214
 
SanDI GG'GwC_CC

Cuts at:      0    1001    1062 
   Size:       1001      61
 
SfcI C'TryA_G

Cuts at:      0     101     873    1062 
   Size:        101     772     189

  Fragments arranged by size: 

                 772     189     101
 
SmaI CCC'GGG

Cuts at:      0     381    1062 
   Size:        381     681
 
SnaBI TAC'GTA

Cuts at:      0     208    1062 
   Size:        208     854
 
StuI AGG'CCT

Cuts at:      0     189    1062 
   Size:        189     873
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     853     975    1054    1062 
   Size:        853     122      79       8

  Fragments arranged by size: 

                 853     122      79       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     584     616    1062 
   Size:        584      32     446

  Fragments arranged by size: 

                 584     446      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      89    1062 
   Size:         89     973
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      79    1062 
   Size:         79     983
 
XmaI C'CCGG_G

Cuts at:      0     379    1062 
   Size:        379     683
 
XmnI GAAnn'nnTTC

Cuts at:      0     460    1062 
   Size:        460     602
 

 Enzymes that do cut:

     AcuI   AflIII     AloI     ApaI     ApoI     AvaI    BanII     BclI
    BglII Bme1580I     BpmI     BsaI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI     BsmI Bsp1286I    BspEI    BsrDI    BstYI    BtgZI     ClaI
     DraI     EaeI     EarI  Eco57MI EcoO109I   HincII     MfeI     MscI
     NspI     PciI     PmlI     PpiI    PpuMI   PspOMI    SanDI     SfcI
     SmaI    SnaBI     StuI    TaqII     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
     XbaI     XhoI     ZraI