(Linear) (Six-base) MAPSORT of: KIEE6359.seq  Check: 2917  from: 1  to: 1185



KIEE6359.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AccI GT'mk_AC

Cuts at:      0     844    1185 
   Size:        844     341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1185 
   Size:        545     640
 
AflIII A'CryG_T

Cuts at:      0     180    1185 
   Size:        180    1005
 
ApoI r'AATT_y

Cuts at:      0     387     511     763    1185 
   Size:        387     124     252     422

  Fragments arranged by size: 

                 422     387     252     124
 
BamHI G'GATC_C

Cuts at:      0     413     879    1185 
   Size:        413     466     306

  Fragments arranged by size: 

                 466     413     306
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     436    1001    1185 
   Size:        436     565     184

  Fragments arranged by size: 

                 565     436     184
 
BbvCI CC'TCA_GC

Cuts at:      0      19    1185 
   Size:         19    1166
 
Bme1580I G_kGCm'C

Cuts at:      0    1128    1185 
   Size:       1128      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     144     416    1185 
   Size:        144     272     769

  Fragments arranged by size: 

                 769     272     144
 
BmtI G_CTAG'C

Cuts at:      0     791    1185 
   Size:        791     394
 
Bpu10I CC'TnA_GC

Cuts at:      0      19    1185 
   Size:         19    1166
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     332     362    1185 
   Size:        332      30     823

  Fragments arranged by size: 

                 823     332      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     419    1185 
   Size:        419     766
 
BseYI C'CCAG_C

Cuts at:      0     783    1185 
   Size:        783     402
 
Bsp1286I G_dGCh'C

Cuts at:      0    1128    1185 
   Size:       1128      57
 
BsrGI T'GTAC_A

Cuts at:      0     151    1185 
   Size:        151    1034
 
BstYI r'GATC_y

Cuts at:      0     413     879    1185 
   Size:        413     466     306

  Fragments arranged by size: 

                 466     413     306
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     841    1185 
   Size:        841     344
 
EaeI y'GGCC_r

Cuts at:      0       2      74     120    1185 
   Size:          2      72      46    1065

  Fragments arranged by size: 

                1065      72      46       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545    1185 
   Size:        545     640
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     814    1185 
   Size:        814     371
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     682     714    1185 
   Size:        682      32     471

  Fragments arranged by size: 

                 682     471      32
 
MfeI C'AATT_G

Cuts at:      0     719    1185 
   Size:        719     466
 
MscI TGG'CCA

Cuts at:      0     122    1185 
   Size:        122    1063
 
NdeI CA'TA_TG

Cuts at:      0    1169    1185 
   Size:       1169      16
 
NheI G'CTAG_C

Cuts at:      0     787    1185 
   Size:        787     398
 
NspI r_CATG'y

Cuts at:      0     184    1185 
   Size:        184    1001
 
PciI A'CATG_T

Cuts at:      0     180    1185 
   Size:        180    1005
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     735    1185 
   Size:        735     450
 
PfoI T'CCnGG_A

Cuts at:      0     849    1185 
   Size:        849     336
 
ScaI AGT'ACT

Cuts at:      0     426    1185 
   Size:        426     759
 
SfcI C'TryA_G

Cuts at:      0     299    1049    1185 
   Size:        299     750     136

  Fragments arranged by size: 

                 750     299     136
 
SspI AAT'ATT

Cuts at:      0     468    1185 
   Size:        468     717
 
StyI C'CwwG_G

Cuts at:      0     618    1185 
   Size:        618     567
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     422    1185 
   Size:        422     763
 
TatI w'GTAC_w

Cuts at:      0     151     424     891    1185 
   Size:        151     273     467     294

  Fragments arranged by size: 

                 467     294     273     151
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364    1185 
   Size:        332      32     821

  Fragments arranged by size: 

                 821     332      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1043    1185 
   Size:       1043     142
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI    BamHI     BbsI    BbvCI Bme1580I
     BmrI     BmtI   Bpu10I    BsaXI    BseRI    BseYI Bsp1286I    BsrGI
    BstYI    BtgZI     EaeI  Eco57MI    EcoNI    Hin4I     MfeI     MscI
     NdeI     NheI     NspI     PciI    PflMI     PfoI     ScaI     SfcI
     SspI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI