Restriction map for
pFN21AE6358
(Linear) (Six-base) MAPSORT of: KIEE6358.seq Check: 7188 from: 1 to: 978
KIEE6358.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AflIII A'CryG_T
Cuts at: 0 416 978
Size: 416 562
AhdI GACnn_n'nnGTC
Cuts at: 0 186 978
Size: 186 792
AlwNI CAG_nnn'CTG
Cuts at: 0 555 978
Size: 555 423
ApaLI G'TGCA_C
Cuts at: 0 535 978
Size: 535 443
BanII G_rGCy'C
Cuts at: 0 318 389 978
Size: 318 71 589
Fragments arranged by size:
589 318 71
BglII A'GATC_T
Cuts at: 0 657 978
Size: 657 321
Bme1580I G_kGCm'C
Cuts at: 0 539 589 978
Size: 539 50 389
Fragments arranged by size:
539 389 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 927 978
Size: 927 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 304 373 978
Size: 11 293 69 605
Fragments arranged by size:
605 293 69 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 808 978
Size: 808 170
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 165 978
Size: 165 813
BseYI C'CCAG_C
Cuts at: 0 688 961 978
Size: 688 273 17
Fragments arranged by size:
688 273 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 187 713 978
Size: 76 111 526 265
Fragments arranged by size:
526 265 111 76
BsiHKAI G_wGCw'C
Cuts at: 0 539 978
Size: 539 439
BsmI GAATG_Cn'
Cuts at: 0 282 357 978
Size: 282 75 621
Fragments arranged by size:
621 282 75
Bsp1286I G_dGCh'C
Cuts at: 0 318 389 539 589 978
Size: 318 71 150 50 389
Fragments arranged by size:
389 318 150 71 50
BspHI T'CATG_A
Cuts at: 0 53 978
Size: 53 925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 747 978
Size: 747 231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 978
Size: 534 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 660 978
Size: 255 405 318
Fragments arranged by size:
405 318 255
BstYI r'GATC_y
Cuts at: 0 657 951 978
Size: 657 294 27
Fragments arranged by size:
657 294 27
BtgI C'CryG_G
Cuts at: 0 683 978
Size: 683 295
BtsI GCAGTG_nn'
Cuts at: 0 214 978
Size: 214 764
DraIII CAC_nnn'GTG
Cuts at: 0 99 978
Size: 99 879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 304 373 978
Size: 11 293 69 605
Fragments arranged by size:
605 293 69 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 622 978
Size: 622 356
EcoO109I rG'GnC_Cy
Cuts at: 0 363 703 978
Size: 363 340 275
Fragments arranged by size:
363 340 275
HaeII r_GCGC'y
Cuts at: 0 492 978
Size: 492 486
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 978
Size: 172 32 774
Fragments arranged by size:
774 172 32
MspA1I CmG'CkG
Cuts at: 0 450 552 978
Size: 450 102 426
Fragments arranged by size:
450 426 102
NcoI C'CATG_G
Cuts at: 0 683 978
Size: 683 295
NdeI CA'TA_TG
Cuts at: 0 114 978
Size: 114 864
NspI r_CATG'y
Cuts at: 0 36 978
Size: 36 942
PpuMI rG'GwC_Cy
Cuts at: 0 363 978
Size: 363 615
PshAI GACnn'nnGTC
Cuts at: 0 130 978
Size: 130 848
PstI C_TGCA'G
Cuts at: 0 449 978
Size: 449 529
PvuII CAG'CTG
Cuts at: 0 450 552 978
Size: 450 102 426
Fragments arranged by size:
450 426 102
SfcI C'TryA_G
Cuts at: 0 319 325 445 978
Size: 319 6 120 533
Fragments arranged by size:
533 319 120 6
SmlI C'TyrA_G
Cuts at: 0 970 978
Size: 970 8
StyI C'CwwG_G
Cuts at: 0 155 683 821 978
Size: 155 528 138 157
Fragments arranged by size:
528 157 155 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 221 978
Size: 221 757
Enzymes that do cut:
AflIII AhdI AlwNI ApaLI BanII BglII Bme1580I BmrI
BpmI BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BstAPI BstXI BstYI BtgI BtsI DraIII
Eco57MI EcoNI EcoO109I HaeII Hin4I MspA1I NcoI NdeI
NspI PpuMI PshAI PstI PvuII SfcI SmlI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI