Restriction map for
pFN21AE6354
(Linear) (Six-base) MAPSORT of: KIEE6354.seq Check: 8139 from: 1 to: 1155
KIEE6354.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AatII G_ACGT'C
Cuts at: 0 49 1155
Size: 49 1106
AccI GT'mk_AC
Cuts at: 0 293 1155
Size: 293 862
Acc65I G'GTAC_C
Cuts at: 0 572 965 1155
Size: 572 393 190
Fragments arranged by size:
572 393 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1155
Size: 302 853
AleI CACnn'nnGTG
Cuts at: 0 410 1155
Size: 410 745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 679 1155
Size: 679 476
AlwNI CAG_nnn'CTG
Cuts at: 0 243 280 1083 1155
Size: 243 37 803 72
Fragments arranged by size:
803 243 72 37
ApaI G_GGCC'C
Cuts at: 0 582 1155
Size: 582 573
AvaI C'yCGr_G
Cuts at: 0 129 1155
Size: 129 1026
BanI G'GyrC_C
Cuts at: 0 11 67 418 572 965 1155
Size: 11 56 351 154 393 190
Fragments arranged by size:
393 351 190 154 56 11
BanII G_rGCy'C
Cuts at: 0 582 938 1155
Size: 582 356 217
Fragments arranged by size:
582 356 217
BbvCI CC'TCA_GC
Cuts at: 0 94 1135 1155
Size: 94 1041 20
Fragments arranged by size:
1041 94 20
BfrBI ATG'CAT
Cuts at: 0 564 1155
Size: 564 591
BglI GCCn_nnn'nGGC
Cuts at: 0 133 683 1155
Size: 133 550 472
Fragments arranged by size:
550 472 133
Bme1580I G_kGCm'C
Cuts at: 0 72 582 961 1155
Size: 72 510 379 194
Fragments arranged by size:
510 379 194 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 268 429 1155
Size: 268 161 726
Fragments arranged by size:
726 268 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 950 1155
Size: 535 415 205
Fragments arranged by size:
535 415 205
Bpu10I CC'TnA_GC
Cuts at: 0 94 1135 1155
Size: 94 1041 20
Fragments arranged by size:
1041 94 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 619 631 1155
Size: 613 6 12 524
Fragments arranged by size:
613 524 12 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 1041 1155
Size: 256 785 114
Fragments arranged by size:
785 256 114
BsaAI yAC'GTr
Cuts at: 0 663 962 1155
Size: 663 299 193
Fragments arranged by size:
663 299 193
BsaHI Gr'CG_yC
Cuts at: 0 46 1155
Size: 46 1109
BsaWI w'CCGG_w
Cuts at: 0 770 1155
Size: 770 385
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 976 1155
Size: 946 30 179
Fragments arranged by size:
946 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 1029 1155
Size: 616 413 126
Fragments arranged by size:
616 413 126
BseYI C'CCAG_C
Cuts at: 0 110 581 731 986 1155
Size: 110 471 150 255 169
Fragments arranged by size:
471 255 169 150 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1013 1155
Size: 702 311 142
Fragments arranged by size:
702 311 142
BsiHKAI G_wGCw'C
Cuts at: 0 551 1155
Size: 551 604
Bsp1286I G_dGCh'C
Cuts at: 0 72 551 582 938 961 1155
Size: 72 479 31 356 23 194
Fragments arranged by size:
479 356 194 72 31 23
BspEI T'CCGG_A
Cuts at: 0 770 1155
Size: 770 385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 560 1155
Size: 94 466 595
Fragments arranged by size:
595 466 94
BsrBI CCG'CTC
Cuts at: 0 305 1155
Size: 305 850
BsrDI GCAATG_nn'
Cuts at: 0 425 1155
Size: 425 730
BsrFI r'CCGG_y
Cuts at: 0 64 203 415 1155
Size: 64 139 212 740
Fragments arranged by size:
740 212 139 64
BssHII G'CGCG_C
Cuts at: 0 25 1155
Size: 25 1130
BstYI r'GATC_y
Cuts at: 0 1055 1155
Size: 1055 100
Bsu36I CC'TnA_GG
Cuts at: 0 340 1155
Size: 340 815
BtgI C'CryG_G
Cuts at: 0 257 356 505 950 1155
Size: 257 99 149 445 205
Fragments arranged by size:
445 257 205 149 99
BtsI GCAGTG_nn'
Cuts at: 0 143 241 456 1126 1155
Size: 143 98 215 670 29
Fragments arranged by size:
670 215 143 98 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 166 1155
Size: 166 989
EarI CTCTTCn'nnn_
Cuts at: 0 1000 1155
Size: 1000 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 535 950 1155
Size: 302 233 415 205
Fragments arranged by size:
415 302 233 205
EcoO109I rG'GnC_Cy
Cuts at: 0 330 345 1014 1080 1106 1155
Size: 330 15 669 66 26 49
Fragments arranged by size:
669 330 66 49 26 15
HaeII r_GCGC'y
Cuts at: 0 39 139 1155
Size: 39 100 1016
Fragments arranged by size:
1016 100 39
HincII GTy'rAC
Cuts at: 0 294 1155
Size: 294 861
KpnI G_GTAC'C
Cuts at: 0 576 969 1155
Size: 576 393 186
Fragments arranged by size:
576 393 186
MslI CAynn'nnrTG
Cuts at: 0 410 1155
Size: 410 745
MspA1I CmG'CkG
Cuts at: 0 585 716 1155
Size: 585 131 439
Fragments arranged by size:
585 439 131
NaeI GCC'GGC
Cuts at: 0 205 417 1155
Size: 205 212 738
Fragments arranged by size:
738 212 205
NcoI C'CATG_G
Cuts at: 0 257 950 1155
Size: 257 693 205
Fragments arranged by size:
693 257 205
NgoMIV G'CCGG_C
Cuts at: 0 203 415 1155
Size: 203 212 740
Fragments arranged by size:
740 212 203
NsiI A_TGCA'T
Cuts at: 0 566 1155
Size: 566 589
NspI r_CATG'y
Cuts at: 0 759 1155
Size: 759 396
PfoI T'CCnGG_A
Cuts at: 0 1102 1155
Size: 1102 53
PmlI CAC'GTG
Cuts at: 0 962 1155
Size: 962 193
PpuMI rG'GwC_Cy
Cuts at: 0 330 345 1106 1155
Size: 330 15 761 49
Fragments arranged by size:
761 330 49 15
PspOMI G'GGCC_C
Cuts at: 0 578 1155
Size: 578 577
PstI C_TGCA'G
Cuts at: 0 105 1155
Size: 105 1050
SalI G'TCGA_C
Cuts at: 0 292 1155
Size: 292 863
SanDI GG'GwC_CC
Cuts at: 0 345 1155
Size: 345 810
SapI GCTCTTCn'nnn_
Cuts at: 0 1000 1155
Size: 1000 155
SexAI A'CCwGG_T
Cuts at: 0 968 1155
Size: 968 187
SfcI C'TryA_G
Cuts at: 0 101 1030 1155
Size: 101 929 125
Fragments arranged by size:
929 125 101
SgrAI Cr'CCGG_yG
Cuts at: 0 64 1155
Size: 64 1091
SmaI CCC'GGG
Cuts at: 0 131 1155
Size: 131 1024
SmlI C'TyrA_G
Cuts at: 0 628 634 646 1155
Size: 628 6 12 509
Fragments arranged by size:
628 509 12 6
StyI C'CwwG_G
Cuts at: 0 182 257 743 938 950 1155
Size: 182 75 486 195 12 205
Fragments arranged by size:
486 205 195 182 75 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 429 588 913 1035 1155
Size: 250 179 159 325 122 120
Fragments arranged by size:
325 250 179 159 122 120
TatI w'GTAC_w
Cuts at: 0 650 1155
Size: 650 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 135 167 946 978 1155
Size: 135 32 779 32 177
Fragments arranged by size:
779 177 135 32 32
XmaI C'CCGG_G
Cuts at: 0 129 1155
Size: 129 1026
ZraI GAC'GTC
Cuts at: 0 47 1155
Size: 47 1108
Enzymes that do cut:
AatII AccI Acc65I AcuI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbvCI BfrBI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BstYI Bsu36I BtgI BtsI DrdI
EarI Eco57MI EcoO109I HaeII HincII KpnI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PfoI PmlI PpuMI
PspOMI PstI SalI SanDI SapI SexAI SfcI SgrAI
SmaI SmlI StyI TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BcgI BciVI BclI BglII BlpI BmgBI
BmtI BplI BsaBI BsiEI BsiWI BsmI BsmBI BspHI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SbfI ScaI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmnI