Restriction map for
pFN21AE6353
(Linear) (Six-base) MAPSORT of: KIEE6353.seq Check: 6071 from: 1 to: 1164
KIEE6353.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
Acc65I G'GTAC_C
Cuts at: 0 883 1164
Size: 883 281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1055 1070 1164
Size: 683 372 15 94
Fragments arranged by size:
683 372 94 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 245 1164
Size: 245 919
AlwNI CAG_nnn'CTG
Cuts at: 0 19 1137 1164
Size: 19 1118 27
Fragments arranged by size:
1118 27 19
ApaI G_GGCC'C
Cuts at: 0 608 975 1164
Size: 608 367 189
Fragments arranged by size:
608 367 189
ApaLI G'TGCA_C
Cuts at: 0 709 1164
Size: 709 455
AvaI C'yCGr_G
Cuts at: 0 53 1164
Size: 53 1111
BamHI G'GATC_C
Cuts at: 0 835 1164
Size: 835 329
BanI G'GyrC_C
Cuts at: 0 67 413 532 883 933 1164
Size: 67 346 119 351 50 231
Fragments arranged by size:
351 346 231 119 67 50
BanII G_rGCy'C
Cuts at: 0 8 52 110 119 608 671 975 1164
Size: 8 44 58 9 489 63 304 189
Fragments arranged by size:
489 304 189 63 58 44 9 8
BbeI G_GCGC'C
Cuts at: 0 71 1164
Size: 71 1093
BbsI GAAGACnn'nnnn_
Cuts at: 0 246 1164
Size: 246 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 548 1164
Size: 514 34 616
Fragments arranged by size:
616 514 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 912 1164
Size: 912 252
BglI GCCn_nnn'nGGC
Cuts at: 0 1045 1164
Size: 1045 119
Bme1580I G_kGCm'C
Cuts at: 0 608 713 831 975 1164
Size: 608 105 118 144 189
Fragments arranged by size:
608 189 144 118 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 1125 1164
Size: 1125 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1071 1164
Size: 670 401 93
Fragments arranged by size:
670 401 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 456 1164
Size: 25 431 708
Fragments arranged by size:
708 431 25
BsaAI yAC'GTr
Cuts at: 0 714 1164
Size: 714 450
BsaHI Gr'CG_yC
Cuts at: 0 68 1164
Size: 68 1096
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 667 1164
Size: 142 525 497
Fragments arranged by size:
525 497 142
BseYI C'CCAG_C
Cuts at: 0 19 262 601 717 1164
Size: 19 243 339 116 447
Fragments arranged by size:
447 339 243 116 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 577 946 986 1077 1164
Size: 490 87 369 40 91 87
Fragments arranged by size:
490 369 91 87 87 40
BsiHKAI G_wGCw'C
Cuts at: 0 713 1164
Size: 713 451
BsmBI CGTCTCn'nnnn_
Cuts at: 0 851 1164
Size: 851 313
Bsp1286I G_dGCh'C
Cuts at: 0 8 52 110 119 608 671 713 831
Size: 8 44 58 9 489 63 42 118
Cuts at: 831 975 1164
Size: 144 189
Fragments arranged by size:
489 189 144 118 63 58 44 42
9 8
BspHI T'CATG_A
Cuts at: 0 305 1164
Size: 305 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1092 1164
Size: 1092 72
BsrBI CCG'CTC
Cuts at: 0 844 1164
Size: 844 320
BsrDI GCAATG_nn'
Cuts at: 0 900 1164
Size: 900 264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 565 640 1164
Size: 565 75 524
Fragments arranged by size:
565 524 75
BstBI TT'CG_AA
Cuts at: 0 867 905 1164
Size: 867 38 259
Fragments arranged by size:
867 259 38
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 141 1164
Size: 42 99 1023
Fragments arranged by size:
1023 99 42
BstYI r'GATC_y
Cuts at: 0 835 1164
Size: 835 329
Bsu36I CC'TnA_GG
Cuts at: 0 125 1164
Size: 125 1039
BtgI C'CryG_G
Cuts at: 0 60 618 1164
Size: 60 558 546
Fragments arranged by size:
558 546 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 540 1164
Size: 540 624
BtsI GCAGTG_nn'
Cuts at: 0 159 1164
Size: 159 1005
EaeI y'GGCC_r
Cuts at: 0 299 1010 1164
Size: 299 711 154
Fragments arranged by size:
711 299 154
EarI CTCTTCn'nnn_
Cuts at: 0 85 103 364 659 820 1164
Size: 85 18 261 295 161 344
Fragments arranged by size:
344 295 261 161 85 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 170 346 487 1164
Size: 170 176 141 677
Fragments arranged by size:
677 176 170 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 683 1055 1070 1071 1164
Size: 670 13 372 15 1 93
Fragments arranged by size:
670 372 93 15 13 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 1164
Size: 124 1040
EcoO109I rG'GnC_Cy
Cuts at: 0 171 605 658 723 972 1164
Size: 171 434 53 65 249 192
Fragments arranged by size:
434 249 192 171 65 53
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 114 1164
Size: 114 1050
HaeII r_GCGC'y
Cuts at: 0 71 1164
Size: 71 1093
KasI G'GCGC_C
Cuts at: 0 67 1164
Size: 67 1097
KpnI G_GTAC'C
Cuts at: 0 887 1164
Size: 887 277
MscI TGG'CCA
Cuts at: 0 301 1012 1164
Size: 301 711 152
Fragments arranged by size:
711 301 152
MspA1I CmG'CkG
Cuts at: 0 19 62 648 1027 1091 1164
Size: 19 43 586 379 64 73
Fragments arranged by size:
586 379 73 64 43 19
NarI GG'CG_CC
Cuts at: 0 68 1164
Size: 68 1096
NspI r_CATG'y
Cuts at: 0 214 1164
Size: 214 950
PasI CC'CwG_GG
Cuts at: 0 678 1164
Size: 678 486
PflMI CCAn_nnn'nTGG
Cuts at: 0 19 327 1164
Size: 19 308 837
Fragments arranged by size:
837 308 19
PmlI CAC'GTG
Cuts at: 0 714 1164
Size: 714 450
PpuMI rG'GwC_Cy
Cuts at: 0 171 723 1164
Size: 171 552 441
Fragments arranged by size:
552 441 171
PspOMI G'GGCC_C
Cuts at: 0 604 971 1164
Size: 604 367 193
Fragments arranged by size:
604 367 193
PstI C_TGCA'G
Cuts at: 0 734 1103 1164
Size: 734 369 61
Fragments arranged by size:
734 369 61
PvuII CAG'CTG
Cuts at: 0 1091 1164
Size: 1091 73
SacII CC_GC'GG
Cuts at: 0 63 1164
Size: 63 1101
SanDI GG'GwC_CC
Cuts at: 0 171 1164
Size: 171 993
SapI GCTCTTCn'nnn_
Cuts at: 0 659 1164
Size: 659 505
SbfI CC_TGCA'GG
Cuts at: 0 1103 1164
Size: 1103 61
SfcI C'TryA_G
Cuts at: 0 730 1099 1164
Size: 730 369 65
Fragments arranged by size:
730 369 65
SfoI GGC'GCC
Cuts at: 0 69 1164
Size: 69 1095
SmaI CCC'GGG
Cuts at: 0 55 1164
Size: 55 1109
SphI G_CATG'C
Cuts at: 0 214 1164
Size: 214 950
StyI C'CwwG_G
Cuts at: 0 831 1164
Size: 831 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 1164
Size: 325 839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 576 1164
Size: 576 588
XmaI C'CCGG_G
Cuts at: 0 53 1164
Size: 53 1111
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BglI Bme1580I
BmrI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII KasI
KpnI MscI MspA1I NarI NspI PasI PflMI PmlI
PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI
SfcI SfoI SmaI SphI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BsrFI BsrGI BssHII BssSI BstEII BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI